DOI: 10.1073/pnas.0500812102. Independence of replisomes in <i>Escherichia coli</i> chromosomal replication

Independence of replisomes in Escherichia coli chromosomal replication

10.1073/pnas.0500812102

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

In Escherichia coli DNA replication is carried out by the coordinated action of the proteins within a replisome. After replication initiation, the two bidirectionally oriented replisomes from a single origin are colocalized into higher-order structures termed replication factories. The factory model postulated that the two replisomes are also functionally coupled. We tested this hypothesis by using DNA combing and whole-genome microarrays. Nascent DNA surrounding oriC in single, combed chromosomes showed instead that one replisome, usually the leftward one, was significantly ahead of the other 70% of the time. We next used microarrays to follow replication throughout the genome by measuring DNA copy number. We found in multiple E. coli strains that the replisomes are independent, with the leftward replisome ahead of the rightward one. The size of the bias was strain-specific, varying from 50 to 130 kb in the array results. When we artificially blocked one replisome, the other continued unabated, again demonstrating independence. We suggest an improved version of the factory model that retains the advantages of threading DNA through colocalized replisomes at about equal rates, but allows the cell flexibility to overcome obstacles encountered during elongation.

Bibliography

Breier, A. M., Weier, H.-U. G., & Cozzarelli, N. R. (2005). Independence of replisomes in Escherichia coli chromosomal replication. Proceedings of the National Academy of Sciences, 102(11), 3942â3947.

Authors 3

Adam M. Breier (first)
Heinz-Ulrich G. Weier (additional)
Nicholas R. Cozzarelli (additional)

References 47 Referenced 94

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Dates

Type	When
Created	20 years, 6 months ago (Feb. 28, 2005, 8:24 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 10:05 a.m.)
Indexed	1 month, 2 weeks ago (July 22, 2025, 6:52 a.m.)
Issued	20 years, 6 months ago (Feb. 28, 2005)
Published	20 years, 6 months ago (Feb. 28, 2005)
Published Online	20 years, 6 months ago (Feb. 28, 2005)
Published Print	20 years, 5 months ago (March 15, 2005)

Funders 0

None

BibTeX

@article{Breier_2005, title={Independence of replisomes in Escherichia coli chromosomal replication}, volume={102}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0500812102}, DOI={10.1073/pnas.0500812102}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Breier, Adam M. and Weier, Heinz-Ulrich G. and Cozzarelli, Nicholas R.}, year={2005}, month=feb, pages={3942–3947} }

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