DOI: 10.1073/pnas.0500711102. Syne proteins anchor muscle nuclei at the neuromuscular junction

Syne proteins anchor muscle nuclei at the neuromuscular junction

10.1073/pnas.0500711102

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Vertebrate skeletal muscle fibers contain hundreds of nuclei, of which three to six are functionally specialized and stably anchored beneath the postsynaptic membrane at the neuromuscular junction (NMJ). The mechanisms that localize synaptic nuclei and the roles they play in neuromuscular development are unknown. Syne-1 is concentrated at the nuclear envelope of synaptic nuclei; its Caenorhabditis elegans orthologue ANC-1 functions to tether nuclei to the cytoskeleton. To test the involvement of Syne proteins in nuclear anchoring, we generated transgenic mice overexpressing the conserved C-terminal Klarsicht/ANC-1/Syne homology domain of Syne-1. The transgene acted in a dominant interfering fashion, displacing endogenous Syne-1 from the nuclear envelope. Muscle nuclei failed to aggregate at the NMJ in transgenic mice, demonstrating that localization and positioning of synaptic nuclei require Syne proteins. We then exploited this phenotype to show that synaptic nuclear aggregates are dispensable for maturation of the NMJ.

Bibliography

Grady, R. M., Starr, D. A., Ackerman, G. L., Sanes, J. R., & Han, M. (2005). Syne proteins anchor muscle nuclei at the neuromuscular junction. Proceedings of the National Academy of Sciences, 102(12), 4359â4364.

Authors 5

R. Mark Grady (first)
Daniel A. Starr (additional)
Gail L. Ackerman (additional)
Joshua R. Sanes (additional)
Min Han (additional)

References 29 Referenced 190

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Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 8:24 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 12:39 p.m.)
Indexed	1 month, 3 weeks ago (July 11, 2025, 6:21 a.m.)
Issued	20 years, 5 months ago (March 4, 2005)
Published	20 years, 5 months ago (March 4, 2005)
Published Online	20 years, 5 months ago (March 4, 2005)
Published Print	20 years, 5 months ago (March 22, 2005)

Funders 0

None

BibTeX

@article{Grady_2005, title={Syne proteins anchor muscle nuclei at the neuromuscular junction}, volume={102}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0500711102}, DOI={10.1073/pnas.0500711102}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Grady, R. Mark and Starr, Daniel A. and Ackerman, Gail L. and Sanes, Joshua R. and Han, Min}, year={2005}, month=mar, pages={4359–4364} }

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