DOI: 10.1073/pnas.0436796100. Evidence for rotation of V <sub>1</sub> -ATPase

10.1073/pnas.0436796100

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

V o V 1 -ATPase is responsible for acidification of eukaryotic intracellular compartments and ATP synthesis of Archaea and some eubacteria. From the similarity to F o F 1 -ATP synthase, V o V 1 -ATPase has been assumed to be a rotary motor, but to date there are no experimental data to support this. Here we visualized the rotation of single molecules of V 1 -ATPase, a catalytic subcomplex of V o V 1 -ATPase. V 1 -ATPase from Thermus thermophilus was immobilized onto a glass surface, and a bead was attached to the D or F subunit through the biotin-streptavidin linkage. In both cases we observed ATP-dependent rotations of beads, the direction of which was always counterclockwise viewed from the membrane side. Given that three ATP molecules are hydrolyzed per one revolution, rates of rotation agree consistently with rates of ATP hydrolysis at saturating ATP concentrations. This study provides experimental evidence that V o V 1 -ATPase is a rotary motor and that both D and F subunits constitute a rotor shaft.

Bibliography

Imamura, H., Nakano, M., Noji, H., Muneyuki, E., Ohkuma, S., Yoshida, M., & Yokoyama, K. (2003). Evidence for rotation of V 1 -ATPase. Proceedings of the National Academy of Sciences, 100(5), 2312â2315.

Authors 7

Hiromi Imamura (first)
Masahiro Nakano (additional)
Hiroyuki Noji (additional)
Eiro Muneyuki (additional)
Shoji Ohkuma (additional)
Masasuke Yoshida (additional)
Ken Yokoyama (additional)

References 18 Referenced 173

Dates

Type	When
Created	22 years, 5 months ago (March 4, 2003, 1:36 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 2:23 p.m.)
Indexed	1 week, 3 days ago (Aug. 23, 2025, 12:59 a.m.)
Issued	22 years, 6 months ago (Feb. 21, 2003)
Published	22 years, 6 months ago (Feb. 21, 2003)
Published Online	22 years, 6 months ago (Feb. 21, 2003)
Published Print	22 years, 5 months ago (March 4, 2003)

Funders 0

None

BibTeX

@article{Imamura_2003, title={Evidence for rotation of V 1 -ATPase}, volume={100}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0436796100}, DOI={10.1073/pnas.0436796100}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Imamura, Hiromi and Nakano, Masahiro and Noji, Hiroyuki and Muneyuki, Eiro and Ohkuma, Shoji and Yoshida, Masasuke and Yokoyama, Ken}, year={2003}, month=feb, pages={2312–2315} }

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