DOI: 10.1073/pnas.042678299. Marrow stromal cells form guiding strands in the injured spinal cord and promote recovery

Marrow stromal cells form guiding strands in the injured spinal cord and promote recovery

10.1073/pnas.042678299

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Marrow stromal cells (MSC) can be expanded rapidly in vitro and differentiated into multiple mesodermal cell types. In addition, differentiation into neuron-like cells expressing markers typical for mature neurons has been reported. To analyze whether such cells, exposed to differentiation media, could develop electrophysiological properties characteristic of neurons, we performed whole-cell recordings. Neuron-like MSC, however, lacked voltage-gated ion channels necessary for generation of action potentials. We then delivered MSC into the injured spinal cord to study the fate of transplanted MSC and possible effects on functional outcome in animals rendered paraplegic. MSC given 1 week after injury led to significantly larger numbers of surviving cells than immediate treatment and significant improvements of gait. Histology 5 weeks after spinal cord injury revealed that MSC were tightly associated with longitudinally arranged immature astrocytes and formed bundles bridging the epicenter of the injury. Robust bundles of neurofilament-positive fibers and some 5-hydroxytryptamine-positive fibers were found mainly at the interface between graft and scar tissue. MSC constitute an easily accessible, easily expandable source of cells that may prove useful in the establishment of spinal cord repair protocols.

Bibliography

Hofstetter, C. P., Schwarz, E. J., Hess, D., Widenfalk, J., El Manira, A., Prockop, D. J., & Olson, L. (2002). Marrow stromal cells form guiding strands in the injured spinal cord and promote recovery. Proceedings of the National Academy of Sciences, 99(4), 2199â2204.

Authors 7

C. P. Hofstetter (first)
E. J. Schwarz (additional)
D. Hess (additional)
J. Widenfalk (additional)
A. El Manira (additional)
Darwin J. Prockop (additional)
L. Olson (additional)

References 44 Referenced 788

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:36 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 2:27 p.m.)
Indexed	4 days, 23 hours ago (Aug. 23, 2025, 9:15 p.m.)
Issued	23 years, 6 months ago (Feb. 19, 2002)
Published	23 years, 6 months ago (Feb. 19, 2002)
Published Online	23 years, 6 months ago (Feb. 19, 2002)
Published Print	23 years, 6 months ago (Feb. 19, 2002)

Funders 0

None

BibTeX

@article{Hofstetter_2002, title={Marrow stromal cells form guiding strands in the injured spinal cord and promote recovery}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.042678299}, DOI={10.1073/pnas.042678299}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hofstetter, C. P. and Schwarz, E. J. and Hess, D. and Widenfalk, J. and El Manira, A. and Prockop, Darwin J. and Olson, L.}, year={2002}, month=feb, pages={2199–2204} }

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