DOI: 10.1073/pnas.041614998. Nanohedra: Using symmetry to design self assembling protein cages, layers, crystals, and filaments

Nanohedra: Using symmetry to design self assembling protein cages, layers, crystals, and filaments

10.1073/pnas.041614998

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A general strategy is described for designing proteins that self assemble into large symmetrical nanomaterials, including molecular cages, filaments, layers, and porous materials. In this strategy, one molecule of protein A, which naturally forms a self-assembling oligomer, A n , is fused rigidly to one molecule of protein B, which forms another self-assembling oligomer, B m . The result is a fusion protein, A-B, which self assembles with other identical copies of itself into a designed nanohedral particle or material, (A-B) p . The strategy is demonstrated through the design, production, and characterization of two fusion proteins: a 49-kDa protein designed to assemble into a cage approximately 15 nm across, and a 44-kDa protein designed to assemble into long filaments approximately 4 nm wide. The strategy opens a way to create a wide variety of potentially useful protein-based materials, some of which share similar features with natural biological assemblies.

Bibliography

Padilla, J. E., Colovos, C., & Yeates, T. O. (2001). Nanohedra: Using symmetry to design self assembling protein cages, layers, crystals, and filaments. Proceedings of the National Academy of Sciences, 98(5), 2217â2221.

Authors 3

Jennifer E. Padilla (first)
Christos Colovos (additional)
Todd O. Yeates (additional)

References 30 Referenced 427

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:45 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 12:37 p.m.)
Indexed	56 minutes ago (Aug. 30, 2025, 2:40 a.m.)
Issued	24 years, 6 months ago (Feb. 20, 2001)
Published	24 years, 6 months ago (Feb. 20, 2001)
Published Online	24 years, 6 months ago (Feb. 20, 2001)
Published Print	24 years, 6 months ago (Feb. 27, 2001)

Funders 0

None

BibTeX

@article{Padilla_2001, title={Nanohedra: Using symmetry to design self assembling protein cages, layers, crystals, and filaments}, volume={98}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.041614998}, DOI={10.1073/pnas.041614998}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Padilla, Jennifer E. and Colovos, Christos and Yeates, Todd O.}, year={2001}, month=feb, pages={2217–2221} }

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