DOI: 10.1073/pnas.0409178102. Retrovirus envelope protein complex structure <i>in situ</i> studied by cryo-electron tomography

Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography

10.1073/pnas.0409178102

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We used cryo-electron tomography in conjunction with single-particle averaging techniques to study the structures of frozen-hydrated envelope glycoprotein (Env) complexes on intact Moloney murine leukemia retrovirus particles. Cryo-electron tomography allows 3D imaging of viruses in toto at a resolution sufficient to locate individual macromolecules, and local averaging of abundant complexes substantially improves the resolution. The averaging of repetitive features in electron tomograms is hampered by a low signal-to-noise ratio and anisotropic resolution, which results from the “missing-wedge” effect. We developed an iterative 3D averaging algorithm that compensates for this effect and used it to determine the trimeric structure of Env to a resolution of 2.7 nm, at which individual domains can be resolved. Strikingly, the 3D reconstruction is shaped like a tripod in which the trimer penetrates the membrane at three distinct locations ≈4.5 nm apart from one another. The Env reconstruction allows tentative docking of the x-ray crystal structure of the receptor-binding domain. This study thus provides 3D structural information regarding the prefusion conformation of an intact unstained retrovirus surface protein.

Bibliography

FÃ¶rster, F., Medalia, O., Zauberman, N., Baumeister, W., & Fass, D. (2005). Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography. Proceedings of the National Academy of Sciences, 102(13), 4729â4734.

Authors 5

Friedrich Förster (first)
Ohad Medalia (additional)
Nathan Zauberman (additional)
Wolfgang Baumeister (additional)
Deborah Fass (additional)

References 38 Referenced 297

Dates

Type	When
Created	20 years, 5 months ago (March 17, 2005, 11:53 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 10:26 a.m.)
Indexed	2 weeks, 5 days ago (Aug. 2, 2025, 1:02 a.m.)
Issued	20 years, 5 months ago (March 17, 2005)
Published	20 years, 5 months ago (March 17, 2005)
Published Online	20 years, 5 months ago (March 17, 2005)
Published Print	20 years, 4 months ago (March 29, 2005)

Funders 0

None

BibTeX

@article{F_rster_2005, title={Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography}, volume={102}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0409178102}, DOI={10.1073/pnas.0409178102}, number={13}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Förster, Friedrich and Medalia, Ohad and Zauberman, Nathan and Baumeister, Wolfgang and Fass, Deborah}, year={2005}, month=mar, pages={4729–4734} }

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