DOI: 10.1073/pnas.0407691102. De novogeneration of antigen-specific CD4+CD25+regulatory T cells from human CD4+CD25

De novogeneration of antigen-specific CD4+CD25+regulatory T cells from human CD4+CD25–cells

10.1073/pnas.0407691102

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Antigen-specificity is a hallmark of adaptive T cell-mediated immune responses. CD4+CD25+FOXP3+regulatory T cells (TR) also require activation through the T cell receptor for function. Although these cells require antigen-specific activation, they are generally able to suppress bystander T cell responses once activated. This raises the possibility that antigen-specific TRmay be useful therapeutically by localizing generalized suppressive activity to tissues expressing select target antigens. Here, we demonstrate that TRspecific for particular peptide-MHC complexes can be generated from human CD4+CD25–T cellsin vitroand isolated by using HLA class II tetramers. Influenza hemagglutinin epitopes were used to generate hemagglutinin-specific TR, which required cognate antigen for activation but which subsequently suppressed noncognate bystander T cell responses as well. These findings have implications for the generation of therapeutic regulatory T cells in disease, and also suggest an important mechanism by which T cells may be regulated at the site of inflammation.

Bibliography

Walker, M. R., Carson, B. D., Nepom, G. T., Ziegler, S. F., & Buckner, J. H. (2005). De novogeneration of antigen-specific CD4+CD25+regulatory T cells from human CD4+CD25âcells. Proceedings of the National Academy of Sciences, 102(11), 4103â4108.

Authors 5

Mindi R. Walker (first)
Bryan D. Carson (additional)
Gerald T. Nepom (additional)
Steven F. Ziegler (additional)
Jane H. Buckner (additional)

References 46 Referenced 226

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Dates

Type	When
Created	20 years, 5 months ago (March 8, 2005, 3:37 a.m.)
Deposited	2 years, 3 months ago (May 2, 2023, 12:05 p.m.)
Indexed	5 months ago (March 29, 2025, 10:55 p.m.)
Issued	20 years, 5 months ago (March 7, 2005)
Published	20 years, 5 months ago (March 7, 2005)
Published Online	20 years, 5 months ago (March 7, 2005)
Published Print	20 years, 5 months ago (March 15, 2005)

Funders 0

None

BibTeX

@article{Walker_2005, title={De novogeneration of antigen-specific CD4+CD25+regulatory T cells from human CD4+CD25–cells}, volume={102}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0407691102}, DOI={10.1073/pnas.0407691102}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Walker, Mindi R. and Carson, Bryan D. and Nepom, Gerald T. and Ziegler, Steven F. and Buckner, Jane H.}, year={2005}, month=mar, pages={4103–4108} }

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