DOI: 10.1073/pnas.0405319101. Atomic structure of a tryptophan-zipper pentamer

Atomic structure of a tryptophan-zipper pentamer

10.1073/pnas.0405319101

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Coiled-coil motifs are ubiquitous mediators of specific protein-protein interactions through the formation of interlocking hydrophobic seams between α-helical chains. Residues that form these seams occur at the first (a) and fourth (d) positions of a characteristic 7-aa repeat and are primarily aliphatic. The potential of aromatic residues to promote helix association in a coiled coil was explored by engineering a “Trp-zipper” protein with Trp residues at all 14 a and d positions. The protein forms a discrete, stable, α-helical pentamer in water at physiological pH. Its 1.45-Å crystal structure reveals a parallel, five-stranded coiled coil, a previously uncharacterized type of “knobs-into-holes” packing interaction between interfacial Trp side chains, and an unusual ≈8-Å-diameter axial channel lined with indole rings that is filled with polyethylene glycol 400 and water and sulfate ion molecules. The engineered Trp-zipper pentamer enlarges current views of coiled-coil assembly, molecular recognition, and protein engineering, and may serve as a soluble model for membrane ion channels.

Bibliography

Liu, J., Yong, W., Deng, Y., Kallenbach, N. R., & Lu, M. (2004). Atomic structure of a tryptophan-zipper pentamer. Proceedings of the National Academy of Sciences, 101(46), 16156â16161.

Authors 5

Jie Liu (first)
Wei Yong (additional)
Yiqun Deng (additional)
Neville R. Kallenbach (additional)
Min Lu (additional)

References 32 Referenced 68

Dates

Type	When
Created	20 years, 9 months ago (Nov. 2, 2004, 9:58 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 8:32 a.m.)
Indexed	1 year ago (Aug. 5, 2024, 11:22 p.m.)
Issued	20 years, 9 months ago (Nov. 1, 2004)
Published	20 years, 9 months ago (Nov. 1, 2004)
Published Online	20 years, 9 months ago (Nov. 1, 2004)
Published Print	20 years, 9 months ago (Nov. 16, 2004)

Funders 0

None

BibTeX

@article{Liu_2004, title={Atomic structure of a tryptophan-zipper pentamer}, volume={101}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0405319101}, DOI={10.1073/pnas.0405319101}, number={46}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Liu, Jie and Yong, Wei and Deng, Yiqun and Kallenbach, Neville R. and Lu, Min}, year={2004}, month=nov, pages={16156–16161} }

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