DOI: 10.1073/pnas.0405211101. Identification of a SulP-type bicarbonate transporter in marine cyanobacteria

Identification of a SulP-type bicarbonate transporter in marine cyanobacteria

10.1073/pnas.0405211101

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Cyanobacteria possess a highly effective CO 2 -concentrating mechanism that elevates CO 2 concentrations around the primary carboxylase, Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase). This CO 2 -concentrating mechanism incorporates light-dependent, active uptake systems for CO 2 and HCO – 3 . Through mutant studies in a coastal marine cyanobacterium, Synechococcus sp. strain PCC7002, we identified bicA as a gene that encodes a class of HCO – 3 transporter with relatively low transport affinity, but high flux rate. BicA is widely represented in genomes of oceanic cyanobacteria and belongs to a large family of eukaryotic and prokaryotic transporters presently annotated as sulfate transporters or permeases in many bacteria (SulP family). Further gain-of-function experiments in the freshwater cyanobacterium Synechococcus PCC7942 revealed that bicA expression alone is sufficient to confer a Na + -dependent, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} \begin{equation*}{\mathrm{HCO}}_{3}^{-}\end{equation*}\end{document} uptake activity. We identified and characterized three cyanobacterial BicA transporters in this manner, including one from the ecologically important oceanic strain, Synechococcus WH8102. This study presents functional data concerning prokaryotic members of the SulP transporter family and represents a previously uncharacterized transport function for the family. The discovery of BicA has significant implications for understanding the important contribution of oceanic strains of cyanobacteria to global CO 2 sequestration processes.

Bibliography

Price, G. D., Woodger, F. J., Badger, M. R., Howitt, S. M., & Tucker, L. (2004). Identification of a SulP-type bicarbonate transporter in marine cyanobacteria. Proceedings of the National Academy of Sciences, 101(52), 18228â18233.

Authors 5

G. Dean Price (first)
Fiona J. Woodger (additional)
Murray R. Badger (additional)
Susan M. Howitt (additional)
Loraine Tucker (additional)

References 29 Referenced 267

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Dates

Type	When
Created	20 years, 8 months ago (Dec. 28, 2004, 6:33 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 9:25 a.m.)
Indexed	1 week ago (Aug. 28, 2025, 8:04 a.m.)
Issued	20 years, 8 months ago (Dec. 13, 2004)
Published	20 years, 8 months ago (Dec. 13, 2004)
Published Online	20 years, 8 months ago (Dec. 13, 2004)
Published Print	20 years, 8 months ago (Dec. 28, 2004)

Funders 0

None

BibTeX

@article{Price_2004, title={Identification of a SulP-type bicarbonate transporter in marine cyanobacteria}, volume={101}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0405211101}, DOI={10.1073/pnas.0405211101}, number={52}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Price, G. Dean and Woodger, Fiona J. and Badger, Murray R. and Howitt, Susan M. and Tucker, Loraine}, year={2004}, month=dec, pages={18228–18233} }

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