DOI: 10.1073/pnas.0404640101. A Trim5-cyclophilin A fusion protein found in owl monkey kidney cells can restrict HIV-1

A Trim5-cyclophilin A fusion protein found in owl monkey kidney cells can restrict HIV-1

10.1073/pnas.0404640101

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Lv1 restriction of HIV-1 in the cells of Old World monkeys is associated with the expression of the Trim5 gene. Uniquely, in owl monkey kidney cells, HIV-1 restriction is dependent on the ability of incoming viral capsid protein to bind cyclophilin A (CypA). Cloning of the owl monkey Trim5 gene now reveals the presence of an inserted CypA pseudogene within intron 7 of the Trim5 gene. This insertion results in the formation of a chimeric Trim5-CypA transcript. Transfer of a cDNA corresponding to this transcript into human cells confers cyclosporin A-sensitive resistance to HIV-1 infection. The restriction factor appears to be a chimeric protein created by retrotransposon-mediated exon shuffling.

Bibliography

Nisole, S., Lynch, C., Stoye, J. P., & Yap, M. W. (2004). A Trim5-cyclophilin A fusion protein found in owl monkey kidney cells can restrict HIV-1. Proceedings of the National Academy of Sciences, 101(36), 13324â13328.

Authors 4

Sébastien Nisole (first)
Clare Lynch (additional)
Jonathan P. Stoye (additional)
Melvyn W. Yap (additional)

References 41 Referenced 260

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Dates

Type	When
Created	20 years, 11 months ago (Aug. 23, 2004, 8:16 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 9:06 a.m.)
Indexed	1 month, 1 week ago (July 11, 2025, 9:21 p.m.)
Issued	20 years, 11 months ago (Aug. 23, 2004)
Published	20 years, 11 months ago (Aug. 23, 2004)
Published Online	20 years, 11 months ago (Aug. 23, 2004)
Published Print	20 years, 11 months ago (Sept. 7, 2004)

Funders 0

None

BibTeX

@article{Nisole_2004, title={A Trim5-cyclophilin A fusion protein found in owl monkey kidney cells can restrict HIV-1}, volume={101}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0404640101}, DOI={10.1073/pnas.0404640101}, number={36}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Nisole, Sébastien and Lynch, Clare and Stoye, Jonathan P. and Yap, Melvyn W.}, year={2004}, month=aug, pages={13324–13328} }

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