DOI: 10.1073/pnas.0403545101. Thermophilic ATP synthase has a decamer <i>c</i> -ring: Indication of noninteger 10:3 H <sup>+</sup> /ATP ratio and permissive elastic coupling

Thermophilic ATP synthase has a decamer c -ring: Indication of noninteger 10:3 H + /ATP ratio and permissive elastic coupling

10.1073/pnas.0403545101

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

In a rotary motor F o F 1 -ATP synthase that couples H + transport with ATP synthesis/hydrolysis, it is thought that an F o c subunit oligomer ring ( c -ring) in the membrane rotates as protons pass through F o and a 120° rotation produces one ATP at F 1 . Despite several structural studies, the copy number of F o c subunits in the c -ring has not been determined for any functional F o F 1 . Here, we have generated and isolated thermophilic Bacillus F o F 1 , each containing genetically fused 2-mer–14-mer c ( c 2 – c 14 ). Among them, F o F 1 containing c 2 , c 5 , or c 10 showed ATP-synthesis and other activities. When F 1 was removed, F o containing c 10 worked as an H + channel but F o s containing c 9 , c 11 or c 12 did not. Thus, the c -ring of functional F o F 1 of this organism is a decamer. The inevitable consequence of this finding is noninteger ratios of rotation step sizes of F 1 /F o (120°/36°) and of H + /ATP (10:3). This step-mismatch necessitates elastic twisting of the rotor shaft (and/or the side stalk) during rotation and permissive coupling between unit rotations by H + transport at F o and elementary events in catalysis at F 1 .

Bibliography

Mitome, N., Suzuki, T., Hayashi, S., & Yoshida, M. (2004). Thermophilic ATP synthase has a decamer c -ring: Indication of noninteger 10:3 H + /ATP ratio and permissive elastic coupling. Proceedings of the National Academy of Sciences, 101(33), 12159â12164.

Authors 4

Noriyo Mitome (first)
Toshiharu Suzuki (additional)
Shigehiko Hayashi (additional)
Masasuke Yoshida (additional)

References 28 Referenced 120

10.1146/annurev.biochem.66.1.717
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Suzuki, T., Ueno, H., Mitome, N., Suzuki, J. & Yoshida, M. (2002) J. Biol. Chem. 28, 13281-13285. / J. Biol. Chem. (2002)
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Dates

Type	When
Created	21 years ago (Aug. 9, 2004, 8:16 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 9:29 a.m.)
Indexed	4 weeks, 2 days ago (July 27, 2025, 3:51 a.m.)
Issued	21 years ago (Aug. 9, 2004)
Published	21 years ago (Aug. 9, 2004)
Published Online	21 years ago (Aug. 9, 2004)
Published Print	21 years ago (Aug. 17, 2004)

Funders 0

None

BibTeX

@article{Mitome_2004, title={Thermophilic ATP synthase has a decamer c -ring: Indication of noninteger 10:3 H + /ATP ratio and permissive elastic coupling}, volume={101}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0403545101}, DOI={10.1073/pnas.0403545101}, number={33}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Mitome, Noriyo and Suzuki, Toshiharu and Hayashi, Shigehiko and Yoshida, Masasuke}, year={2004}, month=aug, pages={12159–12164} }

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