DOI: 10.1073/pnas.0403533101. A molecular link between SR protein dephosphorylation and mRNA export

A molecular link between SR protein dephosphorylation and mRNA export

10.1073/pnas.0403533101

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

In metazoans, multiple RNA-binding proteins, including the shuttling serine/arginine-rich (SR)-splicing factors, function as adapters for mRNA nuclear export by interacting with the export receptor TAP/nuclear export factor 1 (NXF1). Yet, it is unclear how interactions between adapters and TAP are regulated. Here, we demonstrate that the SR proteins 9G8 and ASF/SF2 exhibit higher affinity for TAP/NXF1 when hypophosphorylated. 9G8 is recruited to the pre-mRNA in a hyperphosphorylated form but becomes hypophosphorylated during splicing both in vivo and in vitro . TAP preferentially binds spliced mRNA–protein complexes compared with pre-mRNA–protein complexes. Thus, the phosphorylation state of the SR protein adapters may underlie the selectivity of TAP-mediated export of spliced mRNA.

Bibliography

Huang, Y., Yario, T. A., & Steitz, J. A. (2004). A molecular link between SR protein dephosphorylation and mRNA export. Proceedings of the National Academy of Sciences, 101(26), 9666â9670.

Authors 3

Yingqun Huang (first)
Therese A. Yario (additional)
Joan A. Steitz (additional)

References 30 Referenced 224

10.1038/nrm760
10.1016/S0962-8924(03)00106-5
10.1016/S0092-8674(02)00627-X
10.1093/emboj/18.9.2593
Straber, K., Masuda, S., Mason, P., Pfannstiel, J., Oppizzi, M., Rodriguez-Navarro, S., Rondon, A. G., Aguilera, A., Struhl, K., Reed, R. & Hurt, E. (2002) Nature 28, 1-4. / Nature (2002)
10.1038/35098106
10.1083/jcb.200207128
10.1261/rna.5420503
10.1261/rna.5480803
10.1101/gad.12.1.55
10.1016/S1097-2765(01)00233-7
10.1016/S1097-2765(03)00089-3
10.1101/gad.10.10.1233
10.1017/S1355838201002369
10.1017/S1355838200000960
10.1002/j.1460-2075.1994.tb06906.x
Cao, W., Jamison, S. F. & Garcia-Blanco, M. A. (1997) RNA 3, 1456-1467.9404896 / RNA (1997)
10.1128/mcb.12.7.3165-3175.1992
10.1038/35030160
10.1128/MCB.21.21.7307-7319.2001
10.1016/S1097-2765(03)00267-3
10.1083/jcb.143.2.297
10.1038/355730a0
10.1101/gad.11.3.334
10.1074/jbc.275.11.7950
10.1016/S1534-5807(02)00134-X
10.1016/S0092-8674(00)80829-6
10.1016/S1097-2765(04)00030-9
10.1007/978-3-540-44603-3_7
10.1101/gad.1030602

Dates

Type	When
Created	21 years, 2 months ago (June 21, 2004, 8:16 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 9:48 a.m.)
Indexed	2 months, 1 week ago (June 25, 2025, 2:41 p.m.)
Issued	21 years, 2 months ago (June 21, 2004)
Published	21 years, 2 months ago (June 21, 2004)
Published Online	21 years, 2 months ago (June 21, 2004)
Published Print	21 years, 2 months ago (June 29, 2004)

Funders 0

None

BibTeX

@article{Huang_2004, title={A molecular link between SR protein dephosphorylation and mRNA export}, volume={101}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0403533101}, DOI={10.1073/pnas.0403533101}, number={26}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Huang, Yingqun and Yario, Therese A. and Steitz, Joan A.}, year={2004}, month=jun, pages={9666–9670} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        6,
        25
      ]
    ],
    "date-time": "2025-06-25T18:41:38Z",
    "timestamp": 1750876898262
  },
  "reference-count": 30,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "26",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2004,
        6,
        29
      ]
    ]
  },
  "abstract": "<jats:p>\n            In metazoans, multiple RNA-binding proteins, including the shuttling serine/arginine-rich (SR)-splicing factors, function as adapters for mRNA nuclear export by interacting with the export receptor TAP/nuclear export factor 1 (NXF1). Yet, it is unclear how interactions between adapters and TAP are regulated. Here, we demonstrate that the SR proteins 9G8 and ASF/SF2 exhibit higher affinity for TAP/NXF1 when hypophosphorylated. 9G8 is recruited to the pre-mRNA in a hyperphosphorylated form but becomes hypophosphorylated during splicing both\n            <jats:italic>in vivo</jats:italic>\n            and\n            <jats:italic>in vitro</jats:italic>\n            . TAP preferentially binds spliced mRNA\u2013protein complexes compared with pre-mRNA\u2013protein complexes. Thus, the phosphorylation state of the SR protein adapters may underlie the selectivity of TAP-mediated export of spliced mRNA.\n          </jats:p>",
  "DOI": "10.1073/pnas.0403533101",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        6,
        22
      ]
    ],
    "date-time": "2004-06-22T00:16:55Z",
    "timestamp": 1087863415000
  },
  "page": "9666-9670",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 224,
  "title": "A molecular link between SR protein dephosphorylation and mRNA export",
  "prefix": "10.1073",
  "volume": "101",
  "author": [
    {
      "given": "Yingqun",
      "family": "Huang",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520; and Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536"
        }
      ]
    },
    {
      "given": "Therese A.",
      "family": "Yario",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520; and Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536"
        }
      ]
    },
    {
      "given": "Joan A.",
      "family": "Steitz",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520; and Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        2004,
        6,
        21
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_2_1_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nrm760"
    },
    {
      "key": "e_1_3_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0962-8924(03)00106-5"
    },
    {
      "key": "e_1_3_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(02)00627-X"
    },
    {
      "key": "e_1_3_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/18.9.2593"
    },
    {
      "key": "e_1_3_2_5_2",
      "first-page": "1",
      "volume": "28",
      "year": "2002",
      "unstructured": "Straber, K., Masuda, S., Mason, P., Pfannstiel, J., Oppizzi, M., Rodriguez-Navarro, S., Rondon, A. G., Aguilera, A., Struhl, K., Reed, R. & Hurt, E. (2002) Nature 28, 1-4.",
      "journal-title": "Nature"
    },
    {
      "key": "e_1_3_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35098106"
    },
    {
      "key": "e_1_3_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.200207128"
    },
    {
      "key": "e_1_3_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1261/rna.5420503"
    },
    {
      "key": "e_1_3_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1261/rna.5480803"
    },
    {
      "key": "e_1_3_2_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.12.1.55"
    },
    {
      "key": "e_1_3_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(01)00233-7"
    },
    {
      "key": "e_1_3_2_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(03)00089-3"
    },
    {
      "key": "e_1_3_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.10.10.1233"
    },
    {
      "key": "e_1_3_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1017/S1355838201002369"
    },
    {
      "key": "e_1_3_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1017/S1355838200000960"
    },
    {
      "key": "e_1_3_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1994.tb06906.x"
    },
    {
      "key": "e_1_3_2_17_2",
      "first-page": "1456",
      "volume": "3",
      "year": "1997",
      "unstructured": "Cao, W., Jamison, S. F. & Garcia-Blanco, M. A. (1997) RNA 3, 1456-1467.9404896",
      "journal-title": "RNA"
    },
    {
      "key": "e_1_3_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.12.7.3165-3175.1992"
    },
    {
      "key": "e_1_3_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35030160"
    },
    {
      "key": "e_1_3_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.21.21.7307-7319.2001"
    },
    {
      "key": "e_1_3_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(03)00267-3"
    },
    {
      "key": "e_1_3_2_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.143.2.297"
    },
    {
      "key": "e_1_3_2_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/355730a0"
    },
    {
      "key": "e_1_3_2_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.11.3.334"
    },
    {
      "key": "e_1_3_2_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.275.11.7950"
    },
    {
      "key": "e_1_3_2_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1534-5807(02)00134-X"
    },
    {
      "key": "e_1_3_2_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80829-6"
    },
    {
      "key": "e_1_3_2_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(04)00030-9"
    },
    {
      "key": "e_1_3_2_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/978-3-540-44603-3_7"
    },
    {
      "key": "e_1_3_2_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.1030602"
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.0403533101",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        12
      ]
    ],
    "date-time": "2022-04-12T13:48:19Z",
    "timestamp": 1649771299000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.0403533101"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2004,
        6,
        21
      ]
    ]
  },
  "references-count": 30,
  "journal-issue": {
    "issue": "26",
    "published-print": {
      "date-parts": [
        [
          2004,
          6,
          29
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.0403533101"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.0403533101",
  "relation": {
    "has-review": [
      {
        "id-type": "doi",
        "id": "10.3410/f.1019945.227428",
        "asserted-by": "object"
      }
    ]
  },
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        2004,
        6,
        21
      ]
    ]
  },
  "assertion": [
    {
      "value": "2004-06-21",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}