DOI: 10.1073/pnas.0402398101. SecB is a bona fide generalized chaperone in <i>Escherichia coli</i>

SecB is a bona fide generalized chaperone in Escherichia coli

10.1073/pnas.0402398101

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

It is known that the DnaK and Trigger Factor (TF) chaperones cooperate in the folding of newly synthesized cytosolic proteins in Escherichia coli . We recently showed that despite a very narrow temperature range of growth and high levels of aggregated cytosolic proteins, E. coli can tolerate deletion of both chaperones, suggesting that other chaperones might be involved in this process. Here, we show that the secretion-dedicated chaperone SecB efficiently suppresses both the temperature sensitivity and the aggregation-prone phenotypes of a strain lacking both TF and DnaK. SecB suppression is independent of a productive interaction with the SecA subunit of the translocon. Furthermore, in vitro cross-linking experiments demonstrate that SecB can interact both co- and posttranslationally with short nascent chains of both secretory and cytosolic proteins. Finally, we show that such cotranslational substrate recognition by SecB is greatly suppressed in the presence of ribosome-bound TF, but not by DnaK. Taken together, our data demonstrate that SecB acts as a bona fide generalized chaperone.

Bibliography

Ullers, R. S., Luirink, J., Harms, N., Schwager, F., Georgopoulos, C., & Genevaux, P. (2004). SecB is a bona fide generalized chaperone in Escherichia coli. Proceedings of the National Academy of Sciences, 101(20), 7583â7588.

Authors 6

Ronald S. Ullers (first)
Joen Luirink (additional)
Nellie Harms (additional)
Françoise Schwager (additional)
Costa Georgopoulos (additional)
Pierre Genevaux (additional)

References 43 Referenced 99

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Dates

Type	When
Created	21 years, 3 months ago (May 5, 2004, 8:13 p.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 9:47 a.m.)
Indexed	4 weeks, 1 day ago (July 25, 2025, 6:05 a.m.)
Issued	21 years, 3 months ago (May 5, 2004)
Published	21 years, 3 months ago (May 5, 2004)
Published Online	21 years, 3 months ago (May 5, 2004)
Published Print	21 years, 3 months ago (May 18, 2004)

Funders 0

None

BibTeX

@article{Ullers_2004, title={SecB is a bona fide generalized chaperone in Escherichia coli}, volume={101}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0402398101}, DOI={10.1073/pnas.0402398101}, number={20}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Ullers, Ronald S. and Luirink, Joen and Harms, Nellie and Schwager, Françoise and Georgopoulos, Costa and Genevaux, Pierre}, year={2004}, month=may, pages={7583–7588} }

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  "abstract": "<jats:p>\n            It is known that the DnaK and Trigger Factor (TF) chaperones cooperate in the folding of newly synthesized cytosolic proteins in\n            <jats:italic>Escherichia coli</jats:italic>\n            . We recently showed that despite a very narrow temperature range of growth and high levels of aggregated cytosolic proteins,\n            <jats:italic>E. coli</jats:italic>\n            can tolerate deletion of both chaperones, suggesting that other chaperones might be involved in this process. Here, we show that the secretion-dedicated chaperone SecB efficiently suppresses both the temperature sensitivity and the aggregation-prone phenotypes of a strain lacking both TF and DnaK. SecB suppression is independent of a productive interaction with the SecA subunit of the translocon. Furthermore,\n            <jats:italic>in vitro</jats:italic>\n            cross-linking experiments demonstrate that SecB can interact both co- and posttranslationally with short nascent chains of both secretory and cytosolic proteins. Finally, we show that such cotranslational substrate recognition by SecB is greatly suppressed in the presence of ribosome-bound TF, but not by DnaK. Taken together, our data demonstrate that SecB acts as a bona fide generalized chaperone.\n          </jats:p>",
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