DOI: 10.1073/pnas.0401904101. Visualization of ribosome-recycling factor on the <i>Escherichia coli</i> 70S ribosome: Functional implications [Penn]

Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: Functional implications

10.1073/pnas.0401904101

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

After the termination step of protein synthesis, a deacylated tRNA and mRNA remain associated with the ribosome. The ribosome-recycling factor (RRF), together with elongation factor G (EF-G), disassembles this posttermination complex into mRNA, tRNA, and the ribosome. We have obtained a three-dimensional cryo-electron microscopic map of a complex of the Escherichia coli 70S ribosome and RRF. We find that RRF interacts mainly with the segments of the large ribosomal subunit's (50S) rRNA helices that are involved in the formation of two central intersubunit bridges, B2a and B3. The binding of RRF induces considerable conformational changes in some of the functional domains of the ribosome. As compared to its binding position derived previously by hydroxyl radical probing study, we find that RRF binds further inside the intersubunit space of the ribosome such that the tip of its domain I is shifted (by ≈13 Å) toward protein L5 within the central protuberance of the 50S subunit, and domain II is oriented more toward the small ribosomal subunit (30S). Overlapping binding sites of RRF, EF-G, and the P-site tRNA suggest that the binding of EF-G would trigger the removal of deacylated tRNA from the P site by moving RRF toward the ribosomal E site, and subsequent removal of mRNA may be induced by a shift in the position of 16S rRNA helix 44, which harbors part of the mRNA.

Bibliography

Agrawal, R. K., Sharma, M. R., Kiel, M. C., Hirokawa, G., Booth, T. M., Spahn, C. M. T., Grassucci, R. A., Kaji, A., & Frank, J. (2004). Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: Functional implications. Proceedings of the National Academy of Sciences, 101(24), 8900â8905.

Authors 9

Rajendra K. Agrawal (first)
Manjuli R. Sharma (additional)
Michael C. Kiel (additional)
Go Hirokawa (additional)
Timothy M. Booth (additional)
Christian M. T. Spahn (additional)
Robert A. Grassucci (additional)
Akira Kaji (additional)
Joachim Frank (additional)

References 41 Referenced 138

Dates

Type	When
Created	21 years, 2 months ago (June 3, 2004, 8:13 p.m.)
Deposited	3 years, 4 months ago (April 25, 2022, 10:07 p.m.)
Indexed	2 days, 13 hours ago (Aug. 30, 2025, 1:20 p.m.)
Issued	21 years, 2 months ago (June 3, 2004)
Published	21 years, 2 months ago (June 3, 2004)
Published Online	21 years, 2 months ago (June 3, 2004)
Published Print	21 years, 2 months ago (June 15, 2004)

Funders 0

None

BibTeX

@article{Agrawal_2004, title={Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: Functional implications}, volume={101}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0401904101}, DOI={10.1073/pnas.0401904101}, number={24}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Agrawal, Rajendra K. and Sharma, Manjuli R. and Kiel, Michael C. and Hirokawa, Go and Booth, Timothy M. and Spahn, Christian M. T. and Grassucci, Robert A. and Kaji, Akira and Frank, Joachim}, year={2004}, month=jun, pages={8900–8905} }

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