DOI: 10.1073/pnas.032438799. Muscle-derived hematopoietic stem cells are hematopoietic in origin

Muscle-derived hematopoietic stem cells are hematopoietic in origin

10.1073/pnas.032438799

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

It has recently been shown that mononuclear cells from murine skeletal muscle contain the potential to repopulate all major peripheral blood lineages in lethally irradiated mice, but the origin of this activity is unknown. We have fractionated muscle cells on the basis of hematopoietic markers to show that the active population exclusively expresses the hematopoietic stem cell antigens Sca-1 and CD45. Muscle cells obtained from 6- to 8-week-old C57BL/6-CD45.1 mice and enriched for cells expressing Sca-1 and CD45 were able to generate hematopoietic but not myogenic colonies in vitro and repopulated multiple hematopoietic lineages of lethally irradiated C57BL/6-CD45.2 mice. These data show that muscle-derived hematopoietic stem cells are likely derived from the hematopoietic system and are a result not of transdifferentiation of myogenic stem cells but instead of the presence of substantial numbers of hematopoietic stem cells in the muscle. Although CD45-negative cells were highly myogenic in vitro and in vivo , CD45-positive muscle-derived cells displayed only very limited myogenic activity and only in vivo .

Bibliography

McKinney-Freeman, S. L., Jackson, K. A., Camargo, F. D., Ferrari, G., Mavilio, F., & Goodell, M. A. (2002). Muscle-derived hematopoietic stem cells are hematopoietic in origin. Proceedings of the National Academy of Sciences, 99(3), 1341â1346.

Authors 6

Shannon L. McKinney-Freeman (first)
Kathyjo A. Jackson (additional)
Fernando D. Camargo (additional)
Giuliana Ferrari (additional)
Fulvio Mavilio (additional)
Margaret A. Goodell (additional)

References 21 Referenced 351

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:36 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 9:08 a.m.)
Indexed	3 days ago (Sept. 3, 2025, 7:12 a.m.)
Issued	23 years, 7 months ago (Feb. 5, 2002)
Published	23 years, 7 months ago (Feb. 5, 2002)
Published Online	23 years, 7 months ago (Feb. 5, 2002)
Published Print	23 years, 7 months ago (Feb. 5, 2002)

Funders 0

None

BibTeX

@article{McKinney_Freeman_2002, title={Muscle-derived hematopoietic stem cells are hematopoietic in origin}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.032438799}, DOI={10.1073/pnas.032438799}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={McKinney-Freeman, Shannon L. and Jackson, Kathyjo A. and Camargo, Fernando D. and Ferrari, Giuliana and Mavilio, Fulvio and Goodell, Margaret A.}, year={2002}, month=feb, pages={1341–1346} }

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