DOI: 10.1073/pnas.032068299. Paranodal junction formation and spermatogenesis require sulfoglycolipids

Paranodal junction formation and spermatogenesis require sulfoglycolipids

10.1073/pnas.032068299

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Mammalian sulfoglycolipids comprise two major members, sulfatide (HSO 3 -3-galactosylceramide) and seminolipid (HSO 3 -3-monogalactosylalkylacylglycerol). Sulfatide is a major lipid component of the myelin sheath and serves as the epitope for the well known oligodendrocyte-marker antibody O4. Seminolipid is synthesized in spermatocytes and maintained in the subsequent germ cell stages. Both sulfoglycolipids can be synthesized in vitro by using the isolated cerebroside sulfotransferase. To investigate the physiological role of sulfoglycolipids and to determine whether sulfatide and seminolipid are biosynthesized in vivo by a single sulfotransferase, Cst -null mice were generated by gene targeting. Cst −/− mice lacked sulfatide in brain and seminolipid in testis, proving that a single gene copy is responsible for their biosynthesis. Cst −/− mice were born healthy, but began to display hindlimb weakness by 6 weeks of age and subsequently showed a pronounced tremor and progressive ataxia. Although compact myelin was preserved, Cst −/− mice displayed abnormalities in paranodal junctions. On the other hand, Cst −/− males were sterile because of a block in spermatogenesis before the first meiotic division, whereas females were able to breed. These data show a critical role for sulfoglycolipids in myelin function and spermatogenesis.

Bibliography

Honke, K., Hirahara, Y., Dupree, J., Suzuki, K., Popko, B., Fukushima, K., Fukushima, J., Nagasawa, T., Yoshida, N., Wada, Y., & Taniguchi, N. (2002). Paranodal junction formation and spermatogenesis require sulfoglycolipids. Proceedings of the National Academy of Sciences, 99(7), 4227â4232.

Authors 11

Koichi Honke (first)
Yukie Hirahara (additional)
Jeffrey Dupree (additional)
Kinuko Suzuki (additional)
Brian Popko (additional)
Kikuro Fukushima (additional)
Junko Fukushima (additional)
Takashi Nagasawa (additional)
Nobuaki Yoshida (additional)
Yoshinao Wada (additional)
Naoyuki Taniguchi (additional)

References 28 Referenced 286

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10.1038/23488
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10.1093/oxfordjournals.jbchem.a021258
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10.1073/pnas.93.23.13280
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10.1523/JNEUROSCI.19-18-07913.1999

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:37 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 8:53 a.m.)
Indexed	3 weeks, 4 days ago (Aug. 7, 2025, 4:51 a.m.)
Issued	23 years, 5 months ago (March 26, 2002)
Published	23 years, 5 months ago (March 26, 2002)
Published Online	23 years, 5 months ago (March 26, 2002)
Published Print	23 years, 4 months ago (April 2, 2002)

Funders 0

None

BibTeX

@article{Honke_2002, title={Paranodal junction formation and spermatogenesis require sulfoglycolipids}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.032068299}, DOI={10.1073/pnas.032068299}, number={7}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Honke, Koichi and Hirahara, Yukie and Dupree, Jeffrey and Suzuki, Kinuko and Popko, Brian and Fukushima, Kikuro and Fukushima, Junko and Nagasawa, Takashi and Yoshida, Nobuaki and Wada, Yoshinao and Taniguchi, Naoyuki}, year={2002}, month=mar, pages={4227–4232} }

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