DOI: 10.1073/pnas.0308707101. The <i>Vibrio cholerae</i> chitin utilization program

The Vibrio cholerae chitin utilization program

10.1073/pnas.0308707101

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Chitin, an insoluble polymer of GlcNAc, is an abundant source of carbon, nitrogen, and energy for marine microorganisms. Microarray expression profiling and mutational studies of Vibrio cholerae growing on a natural chitin surface, or with the soluble chitin oligosaccharides (GlcNAc) 2–6 , GlcNAc, or the glucosamine dimer (GlcN) 2 identified three sets of differentially regulated genes. We show that ( i ) ChiS, a sensor histidine kinase, regulates expression of the (GlcNAc) 2–6 gene set, including a (GlcNAc) 2 catabolic operon, two extracellular chitinases, a chitoporin, and a PilA-containing type IV pilus, designated ChiRP ( chi tin- r egulated p ilus) that confers a significant growth advantage to V. cholerae on a chitin surface; ( ii ) GlcNAc causes the coordinate expression of genes involved with chitin chemotaxis and adherence and with the transport and assimilation of GlcNAc; ( iii ) (GlcN) 2 induces genes required for the transport and catabolism of nonacetylated chitin residues; and ( iv ) the constitutively expressed MSHA pilus facilitates adhesion to the chitin surface independent of surface chemistry. Collectively, these results provide a global portrait of a complex, multistage V. cholerae program for the efficient utilization of chitin.

Bibliography

Meibom, K. L., Li, X. B., Nielsen, A. T., Wu, C.-Y., Roseman, S., & Schoolnik, G. K. (2004). The Vibrio cholerae chitin utilization program. Proceedings of the National Academy of Sciences, 101(8), 2524â2529.

Authors 6

Karin L. Meibom (first)
Xibing B. Li (additional)
Alex T. Nielsen (additional)
Cheng-Yen Wu (additional)
Saul Roseman (additional)
Gary K. Schoolnik (additional)

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Dates

Type	When
Created	21 years, 6 months ago (Feb. 24, 2004, 11:03 p.m.)
Deposited	3 years, 4 months ago (April 25, 2022, 10:34 p.m.)
Indexed	1 hour, 18 minutes ago (Sept. 2, 2025, 6:36 a.m.)
Issued	21 years, 6 months ago (Feb. 17, 2004)
Published	21 years, 6 months ago (Feb. 17, 2004)
Published Online	21 years, 6 months ago (Feb. 17, 2004)
Published Print	21 years, 6 months ago (Feb. 24, 2004)

Funders 0

None

BibTeX

@article{Meibom_2004, title={The Vibrio cholerae chitin utilization program}, volume={101}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.0308707101}, DOI={10.1073/pnas.0308707101}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Meibom, Karin L. and Li, Xibing B. and Nielsen, Alex T. and Wu, Cheng-Yen and Roseman, Saul and Schoolnik, Gary K.}, year={2004}, month=feb, pages={2524–2529} }

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