DOI: 10.1073/pnas.030421197. Human AML1/MDS1/EVI1 fusion protein induces an acute myelogenous leukemia (AML) in mice: A model for human AML

Human AML1/MDS1/EVI1 fusion protein induces an acute myelogenous leukemia (AML) in mice: A model for human AML

10.1073/pnas.030421197

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The human t(3;21)(q26;q22) translocation is found as a secondary mutation in some cases of chronic myelogenous leukemia during the blast phase and in therapy-related myelodysplasia and acute myelogenous leukemia. One result of this translocation is a fusion between the AML1 , MDS1 , and EVI1 genes, which encodes a transcription factor of approximately 200 kDa. The role of the AML1/MDS1/EVI1 ( AME ) fusion gene in leukemogenesis is largely unknown. In this study, we analyzed the effect of the AME fusion gene in vivo by expressing it in mouse bone marrow cells via retroviral transduction. We found that mice transplanted with AME -transduced bone marrow cells suffered from an acute myelogenous leukemia (AML) 5–13 mo after transplantation. The disease could be readily transferred into secondary recipients with a much shorter latency. Morphological analysis of peripheral blood and bone marrow smears demonstrated the presence of myeloid blast cells and differentiated but immature cells of both myelocytic and monocytic lineages. Cytochemical and flow cytometric analysis confirmed that these mice had a disease similar to the human acute myelomonocytic leukemia. This murine model for AME-induced AML will help dissect the molecular mechanism of AML and the molecular biology of the AML1, MDS1, and EVI1 genes.

Bibliography

Cuenco, G. M., Nucifora, G., & Ren, R. (2000). Human AML1/MDS1/EVI1 fusion protein induces an acute myelogenous leukemia (AML) in mice: A model for human AML. Proceedings of the National Academy of Sciences, 97(4), 1760â1765.

Authors 3

Grace M. Cuenco (first)
Giuseppina Nucifora (additional)
Ruibao Ren (additional)

References 37 Referenced 78

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:37 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 8:41 a.m.)
Indexed	4 months ago (May 4, 2025, 12:06 a.m.)
Issued	25 years, 7 months ago (Feb. 4, 2000)
Published	25 years, 7 months ago (Feb. 4, 2000)
Published Online	25 years, 7 months ago (Feb. 4, 2000)
Published Print	25 years, 6 months ago (Feb. 15, 2000)

Funders 0

None

BibTeX

@article{Cuenco_2000, title={Human AML1/MDS1/EVI1 fusion protein induces an acute myelogenous leukemia (AML) in mice: A model for human AML}, volume={97}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.030421197}, DOI={10.1073/pnas.030421197}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Cuenco, Grace M. and Nucifora, Giuseppina and Ren, Ruibao}, year={2000}, month=feb, pages={1760–1765} }

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