Abstract
Human melanoma cells can be reprogrammed to terminally differentiate and irreversibly lose proliferative capacity by appropriate pharmacological manipulation. Subtraction hybridization identified melanoma differentiation-associated gene-5 (mda-5) as a gene induced during differentiation, cancer reversion, and programmed cell death (apoptosis). This gene contains both a caspase recruitment domain and putative DExH group RNA helicase domains. Atypical helicase motifs of MDA-5 deviate from consensus sequences but are well conserved in a potentially new group of cloned and hypothetical proteins.mda-5is an early response gene inducible by IFN and tumor necrosis factor-α, responding predominantly to IFN-β. Protein kinase C activation by mezerein further augments mda-5 expression induced by IFN-β. Expression ofmda-5is controlled transcriptionally by IFN-β, and the MDA-5 protein localizes in the cytoplasm.mda-5displays RNA-dependent ATPase activity, and ectopic expression ofmda-5in human melanoma cells inhibits colony formation. In these contexts,mda-5may function as a mediator of IFN-induced growth inhibition and/or apoptosis. MDA-5 is a double-stranded RNA-dependent ATPase that contains both a caspase recruitment domain and RNA helicase motifs, with a confirmed association with growth and differentiation in human melanoma cells.
Bibliography
Kang, D., Gopalkrishnan, R. V., Wu, Q., Jankowsky, E., Pyle, A. M., & Fisher, P. B. (2002). mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties. Proceedings of the National Academy of Sciences, 99(2), 637â642.
References
35
Referenced
530
-
M Leszczyniecka, T Robert, P Dent, S Grant, P B Fisher Pharmacol Ther 90, 105–156 (2001).
(
10.1016/S0163-7258(01)00132-2) / Pharmacol Ther by Leszczyniecka M (2001) - S Waxman Differentiation Therapy (Ares-Serono Symposia Publishers, Rome) 10, 1–531 (1996). / Differentiation Therapy by Waxman S (1996)
-
A J Freemerman, J A Vrana, R M Tombes, H Jiang, S P Chellappan, P B Fisher, S Grant Leukemia 11, 504–513 (1997).
(
10.1038/sj.leu.2400625) / Leukemia by Freemerman A J (1997) - H Jiang, J Lin, P B Fisher Mol Cell Differ 2, 221–239 (1994). / Mol Cell Differ by Jiang H (1994)
-
Z Wang, Z Z Su, P B Fisher, S Wang, G VanTuyle, S Grant Exp Cell Res 244, 105–116 (1998).
(
10.1006/excr.1998.4191) / Exp Cell Res by Wang Z (1998) -
P B Fisher, D R Prignoli, H Hermo, I B Weinstein, S Pestka J Interferon Res 5, 11–22 (1985).
(
10.1089/jir.1985.5.11) / J Interferon Res by Fisher P B (1985) - H Jiang, Z-Z Su, J Boyd, P B Fisher Mol Cell Differ 1, 41–66 (1993). / Mol Cell Differ by Jiang H (1993)
- H Jiang, P B Fisher Mol Cell Differ 1, 285–299 (1993). / Mol Cell Differ by Jiang H (1993)
- F M Ausubel, R Brent, R E Kingston, D D Moore, J G Seidman, J A Smith, K Struhl Short Protocols in Molecular Biology (Wiley, New York, 1992). / Short Protocols in Molecular Biology by Ausubel F M (1992)
- H Jiang, J J Lin, Z Z Su, N I Goldstein, P B Fisher Oncogene 11, 2477–2486 (1995). / Oncogene by Jiang H (1995)
-
J D Wagner, E Jankowsky, M Company, A M Pyle, J N Abelson EMBO J 17, 2926–2937 (1998).
(
10.1093/emboj/17.10.2926) / EMBO J by Wagner J D (1998) -
M Kozak Mamm Genome 7, 563–574 (1996).
(
10.1007/s003359900171) / Mamm Genome by Kozak M (1996) -
A Luking, U Stahl, U Schmidt Crit Rev Biochem Mol Biol 33, 259–296 (1998).
(
10.1080/10409239891204233) / Crit Rev Biochem Mol Biol by Luking A (1998) -
E Jankowsky, A Jankowsky Nucleic Acids Res 28, 333–334 (2000).
(
10.1093/nar/28.1.333) / Nucleic Acids Res by Jankowsky E (2000) - Y W Sun Ph.D. thesis (Sanghai Second Medical University, Sanghai, China, 1997). / Ph.D. thesis by Sun Y W (1997)
-
X Zhang, C Wang, L B Schook, R J Hawken, M S Rutherford Microb Pathog 28, 267–278 (2000).
(
10.1006/mpat.1999.0349) / Microb Pathog by Zhang X (2000) -
S E Jacobsen, M P Running, E M Meyerowitz Development (Cambridge, UK) 126, 5231–5243 (1999).
(
10.1242/dev.126.23.5231) / Development (Cambridge, UK) by Jacobsen S E (1999) -
E Bernstein, A A Caudy, S M Hammond, G J Hannon Nature (London) 409, 363–366 (2001).
(
10.1038/35053110) / Nature (London) by Bernstein E (2001) -
K Hofmann, P Bucher, J Tschopp Trends Biochem Sci 22, 155–156 (1997).
(
10.1016/S0968-0004(97)01043-8) / Trends Biochem Sci by Hofmann K (1997) -
J J Chou, H Matsuo, H Duan, G Wagner Cell 94, 171–180 (1998).
(
10.1016/S0092-8674(00)81417-8) / Cell by Chou J J (1998) -
D Kang, H Jiang, Q Wu, S Pestka, P B Fisher Gene 267, 233–242 (2001).
(
10.1016/S0378-1119(01)00384-5) / Gene by Kang D (2001) -
C Garbe, K Krasagakis J Invest Dermatol 100, 239S–244S (1993).
(
10.1038/jid.1993.83) / J Invest Dermatol by Garbe C (1993) -
G R Stark, I M Kerr, B R Williams, R H Silverman, R D Schreiber Annu Rev Biochem 67, 227–264 (1998).
(
10.1146/annurev.biochem.67.1.227) / Annu Rev Biochem by Stark G R (1998) -
C Garbe, K Krasagakis, C C Zouboulis, K Schroder, S Kruger, R Stadler, C E Orfanos J Invest Dermatol 95, 231S–237S (1990).
(
10.1111/1523-1747.ep12875837) / J Invest Dermatol by Garbe C (1990) -
D Guo, J D Dunbar, C H Yang, L M Pfeffer, D B Donner J Immunol 160, 2742–2750 (1998).
(
10.4049/jimmunol.160.6.2742) / J Immunol by Guo D (1998) -
S Grant, K Bhalla, B Weinstein, S Pestka, M D Mileno, P B Fisher Biochem Biophys Res Commun 130, 379–388 (1985).
(
10.1016/0006-291X(85)90428-0) / Biochem Biophys Res Commun by Grant S (1985) -
J W Greiner, J Schlom, S Pestka, J A Langer, P Giacomini, M Kusama, S Ferrone, P B Fisher Pharmacol Ther 31, 209–236 (1985).
(
10.1016/0163-7258(85)90023-3) / Pharmacol Ther by Greiner J W (1985) -
S Balachandran, P C Roberts, T Kipperman, K N Bhalla, R W Compans, D R Archer, G N Barber J Virol 74, 1513–1523 (2000).
(
10.1128/JVI.74.3.1513-1523.2000) / J Virol by Balachandran S (2000) -
D Wallach, E E Varfolomeev, N L Malinin, Y V Goltsev, A V Kovalenko, M P Boldin Annu Rev Immunol 17, 331–367 (1999).
(
10.1146/annurev.immunol.17.1.331) / Annu Rev Immunol by Wallach D (1999) -
T Tamaoki, H Nomoto, I Takahashi, Y Kato, M Morimoto, F Tomita Biochem Biophys Res Commun 135, 397–402 (1986).
(
10.1016/0006-291X(86)90008-2) / Biochem Biophys Res Commun by Tamaoki T (1986) -
A Morreale, B Mallon, G Beale, J Watson, M Rumsby FEBS Lett 417, 38–42 (1997).
(
10.1016/S0014-5793(97)01252-0) / FEBS Lett by Morreale A (1997) -
A Strasser, L O'Connor, V M Dixit Annu Rev Biochem 69, 217–245 (2000).
(
10.1146/annurev.biochem.69.1.217) / Annu Rev Biochem by Strasser A (2000) - A Pause, N Methot, N Sonenberg Mol Cell Biol 13, 6789–6798 (1993). / Mol Cell Biol by Pause A (1993)
-
C H Gross, S Shuman J Virol 70, 1706–1713 (1996).
(
10.1128/jvi.70.3.1706-1713.1996) / J Virol by Gross C H (1996) -
D W Kim, J Kim, Y Gwack, J H Han, J Choe J Virol 71, 9400–9409 (1997).
(
10.1128/jvi.71.12.9400-9409.1997) / J Virol by Kim D W (1997)
Dates
| Type | When |
|---|---|
| Created | 23 years, 1 month ago (July 26, 2002, 10:36 a.m.) |
| Deposited | 2 years, 4 months ago (April 22, 2023, 8:17 a.m.) |
| Indexed | 2 weeks, 2 days ago (Aug. 12, 2025, 6:23 p.m.) |
| Issued | 23 years, 7 months ago (Jan. 22, 2002) |
| Published | 23 years, 7 months ago (Jan. 22, 2002) |
| Published Online | 23 years, 7 months ago (Jan. 22, 2002) |
| Published Print | 23 years, 7 months ago (Jan. 22, 2002) |
@article{Kang_2002, title={mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.022637199}, DOI={10.1073/pnas.022637199}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kang, Dong-chul and Gopalkrishnan, Rahul V. and Wu, Qingping and Jankowsky, Eckhard and Pyle, Anna Marie and Fisher, Paul B.}, year={2002}, month=jan, pages={637–642} }