DOI: 10.1073/pnas.022618199. tRNA elements mediate the assembly of an icosahedral RNA virus

tRNA elements mediate the assembly of an icosahedral RNA virus

10.1073/pnas.022618199

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

tRNAs, the adapter molecules in protein synthesis, also serve as metabolic cofactors and as primers for viral RNA-directed DNA synthesis. The genomic and subgenomic RNAs of some plant viruses have a 3′-terminal tRNA-like structure (TLS) that can accept a specific amino acid and serve as a site for initiation of replication and as a simple telomere. We report a previously undescribed role for the TLS of brome mosaic virus (BMV), and potentially for cellular tRNA, in mediating the assembly of its icosahedral virions. BMV genomic RNAs and subgenomic RNA lacking the TLS failed to assemble into virions when incubated with purified BMV coat protein. Assembly was restored by addition of a 201-nt RNA containing the BMV TLS. TLSs from two other plant viruses as well as tRNAs from wheat germ and yeast were similarly active in the BMV virion assembly reaction, but ribosomal RNA and polyadenylate did not facilitate assembly. Surprisingly, virions assembled from TLS-less BMV RNA in the presence of tRNAs or TLS-containing short RNA did not incorporate the latter molecules. Consistent with a critical role for the BMV TLS in virion assembly, mutations in the BMV genomic RNAs that were designed to disrupt the folding of the TLS also abolished virion assembly. We discuss the likely roles of the TLS in early stages of virion assembly.

Bibliography

Choi, Y. G., Dreher, T. W., & Rao, A. L. N. (2002). tRNA elements mediate the assembly of an icosahedral RNA virus. Proceedings of the National Academy of Sciences, 99(2), 655â660.

Authors 3

Yoon Gi Choi (first)
Theo W. Dreher (additional)
A. L. N. Rao (additional)

References 45 Referenced 92

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C Florentz, R Giegé tRNA: Structure, Biosynthesis and Function, eds D Söll, U L RajBhandary (Am. Soc. Microbiol., Washington, DC), pp. 141–163 (1995). / tRNA: Structure, Biosynthesis and Function by Florentz C (1995)
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10.1073/pnas.84.21.7383
A L N Rao Encyclopedia of Plant Pathology, eds O C Maloy, T D Murray (Wiley, Toronto), pp. 155–158 (2001). / Encyclopedia of Plant Pathology by Rao A L N (2001)
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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:36 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 9:04 a.m.)
Indexed	1 year ago (Aug. 12, 2024, 7:14 a.m.)
Issued	23 years, 7 months ago (Jan. 8, 2002)
Published	23 years, 7 months ago (Jan. 8, 2002)
Published Online	23 years, 7 months ago (Jan. 8, 2002)
Published Print	23 years, 7 months ago (Jan. 22, 2002)

Funders 0

None

BibTeX

@article{Choi_2002, title={tRNA elements mediate the assembly of an icosahedral RNA virus}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.022618199}, DOI={10.1073/pnas.022618199}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Choi, Yoon Gi and Dreher, Theo W. and Rao, A. L. N.}, year={2002}, month=jan, pages={655–660} }

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  "abstract": "<jats:p>tRNAs, the adapter molecules in protein synthesis, also serve as metabolic cofactors and as primers for viral RNA-directed DNA synthesis. The genomic and subgenomic RNAs of some plant viruses have a 3\u2032-terminal tRNA-like structure (TLS) that can accept a specific amino acid and serve as a site for initiation of replication and as a simple telomere. We report a previously undescribed role for the TLS of brome mosaic virus (BMV), and potentially for cellular tRNA, in mediating the assembly of its icosahedral virions. BMV genomic RNAs and subgenomic RNA lacking the TLS failed to assemble into virions when incubated with purified BMV coat protein. Assembly was restored by addition of a 201-nt RNA containing the BMV TLS. TLSs from two other plant viruses as well as tRNAs from wheat germ and yeast were similarly active in the BMV virion assembly reaction, but ribosomal RNA and polyadenylate did not facilitate assembly. Surprisingly, virions assembled from TLS-less BMV RNA in the presence of tRNAs or TLS-containing short RNA did not incorporate the latter molecules. Consistent with a critical role for the BMV TLS in virion assembly, mutations in the BMV genomic RNAs that were designed to disrupt the folding of the TLS also abolished virion assembly. We discuss the likely roles of the TLS in early stages of virion assembly.</jats:p>",
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