DOI: 10.1073/pnas.022042899. Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels <i>in vivo</i>

Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo

10.1073/pnas.022042899

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The elongating, hyperphosphorylated form of RNA polymerase II is associated with the Elongator complex, which has the histone acetyltransferase (HAT) Elp3 as a subunit. Here we show that, in contrast to the isolated Elp3 subunit, the activity of intact Elongator complex is directed specifically toward the amino-terminal tails of histone H3 and H4, and that Elongator can acetylate both core histones and nucleosomal substrates. The predominant acetylation sites are lysine-14 of histone H3 and lysine-8 of histone H4. The three smallest Elongator subunits—Elp4, Elp5, and Elp6—are required for HAT activity, and Elongator binds to both naked and nucleosomal DNA. By using chromatin immunoprecipitation, we show that the levels of multiply acetylated histone H3 and H4 in chromatin are decreased in vivo in yeast cells lacking ELP3 .

Bibliography

Winkler, G. S., Kristjuhan, A., Erdjument-Bromage, H., Tempst, P., & Svejstrup, J. Q. (2002). Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo. Proceedings of the National Academy of Sciences, 99(6), 3517â3522.

Authors 5

G. Sebastiaan Winkler (first)
Arnold Kristjuhan (additional)
Hediye Erdjument-Bromage (additional)
Paul Tempst (additional)
Jesper Q. Svejstrup (additional)

References 36 Referenced 226

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:37 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 8:11 a.m.)
Indexed	1 month ago (July 23, 2025, 8:03 a.m.)
Issued	23 years, 5 months ago (March 19, 2002)
Published	23 years, 5 months ago (March 19, 2002)
Published Online	23 years, 5 months ago (March 19, 2002)
Published Print	23 years, 5 months ago (March 19, 2002)

Funders 0

None

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