DOI: 10.1073/pnas.012585199. Budding yeast Dsk2p is a polyubiquitin-binding protein that can interact with the proteasome

Budding yeast Dsk2p is a polyubiquitin-binding protein that can interact with the proteasome

10.1073/pnas.012585199

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Dsk2p from Saccharomyces cerevisiae belongs to the class of proteins that contain a ubiquitin-like (UbL) domain at the N terminus together with a ubiquitin-associated (UBA) domain at the C terminus. We show here that the C-terminal UBA domain of Dsk2p binds to K48-linked polyubiquitin chains, and the N-terminal UbL domain of Dsk2p interacts with the proteasome. Overexpression of Dsk2p caused the accumulation of large amounts of polyubiquitin, and extragenic suppressors of the Dsk2p-mediated lethality proved to be temperature-sensitive mutations in two proteasome subunits, rpn1 and pre2. K48-linked ubiquitin-dependent degradation was impaired by disruption of the DSK2 gene. These results indicate that Dsk2p is K48-linked polyubiquitin-binding protein and also interacts with the proteasome. We discuss a possible role of adaptor function of Dsk2p via its UbL and UBA domains in the ubiquitin-proteasome pathway.

Bibliography

Funakoshi, M., Sasaki, T., Nishimoto, T., & Kobayashi, H. (2002). Budding yeast Dsk2p is a polyubiquitin-binding protein that can interact with the proteasome. Proceedings of the National Academy of Sciences, 99(2), 745â750.

Authors 4

Minoru Funakoshi (first)
Toru Sasaki (additional)
Takeharu Nishimoto (additional)
Hideki Kobayashi (additional)

References 45 Referenced 200

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:36 a.m.)
Deposited	3 years, 4 months ago (April 12, 2022, 9:02 a.m.)
Indexed	3 months, 1 week ago (May 16, 2025, 2:41 p.m.)
Issued	23 years, 7 months ago (Jan. 22, 2002)
Published	23 years, 7 months ago (Jan. 22, 2002)
Published Online	23 years, 7 months ago (Jan. 22, 2002)
Published Print	23 years, 7 months ago (Jan. 22, 2002)

Funders 0

None

BibTeX

@article{Funakoshi_2002, title={Budding yeast Dsk2p is a polyubiquitin-binding protein that can interact with the proteasome}, volume={99}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.012585199}, DOI={10.1073/pnas.012585199}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Funakoshi, Minoru and Sasaki, Toru and Nishimoto, Takeharu and Kobayashi, Hideki}, year={2002}, month=jan, pages={745–750} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        5,
        16
      ]
    ],
    "date-time": "2025-05-16T18:41:56Z",
    "timestamp": 1747420916790
  },
  "reference-count": 45,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "2",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2002,
        1,
        22
      ]
    ]
  },
  "abstract": "<jats:p>\n            Dsk2p from\n            <jats:italic>Saccharomyces cerevisiae</jats:italic>\n            belongs to the class of proteins that contain a ubiquitin-like (UbL) domain at the N terminus together with a ubiquitin-associated (UBA) domain at the C terminus. We show here that the C-terminal UBA domain of Dsk2p binds to K48-linked polyubiquitin chains, and the N-terminal UbL domain of Dsk2p interacts with the proteasome. Overexpression of Dsk2p caused the accumulation of large amounts of polyubiquitin, and extragenic suppressors of the Dsk2p-mediated lethality proved to be temperature-sensitive mutations in two proteasome subunits,\n            <jats:italic>rpn1</jats:italic>\n            and\n            <jats:italic>pre2.</jats:italic>\n            K48-linked ubiquitin-dependent degradation was impaired by disruption of the\n            <jats:italic>DSK2</jats:italic>\n            gene. These results indicate that Dsk2p is K48-linked polyubiquitin-binding protein and also interacts with the proteasome. We discuss a possible role of adaptor function of Dsk2p via its UbL and UBA domains in the ubiquitin-proteasome pathway.\n          </jats:p>",
  "DOI": "10.1073/pnas.012585199",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        26
      ]
    ],
    "date-time": "2002-07-26T14:36:44Z",
    "timestamp": 1027694204000
  },
  "page": "745-750",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 200,
  "title": "Budding yeast Dsk2p is a polyubiquitin-binding protein that can interact with the proteasome",
  "prefix": "10.1073",
  "volume": "99",
  "author": [
    {
      "given": "Minoru",
      "family": "Funakoshi",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan"
        }
      ]
    },
    {
      "given": "Toru",
      "family": "Sasaki",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan"
        }
      ]
    },
    {
      "given": "Takeharu",
      "family": "Nishimoto",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan"
        }
      ]
    },
    {
      "given": "Hideki",
      "family": "Kobayashi",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan"
        }
      ]
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        2002,
        1,
        22
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_3_3_1_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0962-8924(00)01785-2"
    },
    {
      "key": "e_1_3_3_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.133.6.1331"
    },
    {
      "key": "e_1_3_3_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35661"
    },
    {
      "key": "e_1_3_3_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/genetics/153.1.69"
    },
    {
      "key": "e_1_3_3_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35023547"
    },
    {
      "key": "e_1_3_3_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/18.18.5009"
    },
    {
      "key": "e_1_3_3_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(00)00040-X"
    },
    {
      "key": "e_1_3_3_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.151.4.847"
    },
    {
      "key": "e_1_3_3_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0167-4889(00)00124-5"
    },
    {
      "key": "e_1_3_3_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0955-0674(97)80079-8"
    },
    {
      "key": "e_1_3_3_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.biochem.67.1.425"
    },
    {
      "key": "e_1_3_3_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80929-0"
    },
    {
      "key": "e_1_3_3_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.8.1.171"
    },
    {
      "key": "e_1_3_3_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.273.10.5461"
    },
    {
      "key": "e_1_3_3_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.275.20.15182"
    },
    {
      "key": "e_1_3_3_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0968-0004(00)01681-9"
    },
    {
      "key": "e_1_3_3_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.13.12.7757-7765.1993"
    },
    {
      "key": "e_1_3_3_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/jcs.111.12.1635"
    },
    {
      "key": "e_1_3_3_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0014-5793(00)01135-2"
    },
    {
      "key": "e_1_3_3_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(00)80212-9"
    },
    {
      "key": "e_1_3_3_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/17.21.6135"
    },
    {
      "key": "e_1_3_3_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/genetics/122.1.19"
    },
    {
      "key": "e_1_3_3_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/genetics/152.3.853"
    },
    {
      "key": "e_1_3_3_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.3018930"
    },
    {
      "key": "e_1_3_3_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.149.7.1361"
    },
    {
      "key": "e_1_3_3_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.11.10.3425"
    },
    {
      "key": "e_1_3_3_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.93.22.12142"
    },
    {
      "key": "e_1_3_3_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1987.tb02384.x"
    },
    {
      "key": "e_1_3_3_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/19.1.94"
    },
    {
      "key": "e_1_3_3_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.14.12.7876-7883.1994"
    },
    {
      "key": "e_1_3_3_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.274.38.27299"
    },
    {
      "key": "e_1_3_3_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)00126-4"
    },
    {
      "key": "e_1_3_3_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)00011-8"
    },
    {
      "key": "e_1_3_3_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2443.1997.1230320.x"
    },
    {
      "key": "e_1_3_3_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81603-7"
    },
    {
      "key": "e_1_3_3_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.270.29.17442"
    },
    {
      "key": "e_1_3_3_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/87575"
    },
    {
      "key": "e_1_3_3_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.060025497"
    },
    {
      "key": "e_1_3_3_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/385737a0"
    },
    {
      "key": "e_1_3_3_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.10.8.2583"
    },
    {
      "key": "e_1_3_3_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.16.11.6020"
    },
    {
      "key": "e_1_3_3_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.19.10.6575"
    },
    {
      "key": "e_1_3_3_43_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80574-7"
    },
    {
      "key": "e_1_3_3_44_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.21.6.1997-2007.2001"
    },
    {
      "key": "e_1_3_3_45_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/ncb1001-939"
    }
  ],
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.012585199",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        12
      ]
    ],
    "date-time": "2022-04-12T13:02:57Z",
    "timestamp": 1649768577000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.012585199"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2002,
        1,
        22
      ]
    ]
  },
  "references-count": 45,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          2002,
          1,
          22
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.012585199"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.012585199",
  "relation": {
    "has-review": [
      {
        "id-type": "doi",
        "id": "10.3410/f.1003885.42706",
        "asserted-by": "object"
      }
    ]
  },
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        2002,
        1,
        22
      ]
    ]
  },
  "assertion": [
    {
      "value": "2001-10-24",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2001-11-01",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2002-01-22",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}