DOI: 10.1063/1.476917. Optical properties of protein monolayers during assembly

Optical properties of protein monolayers during assembly

10.1063/1.476917

Crossref journal-article

AIP Publishing

The Journal of Chemical Physics (317)

Abstract

Protein monolayers were built up by allowing protein molecules in solution to deposit at the solid/liquid interface. The structural evolution of these layers was followed using optical waveguide lightmode spectroscopy (OWLS). The measured effective refractive indices were interpreted using the uniform thin film model. For a relatively rigid, spheroidal protein (transferrin), the thickness of the layer was already established at very low surface coverages, and the refractive index increased during deposition. For a highly elongated and flexible protein (fibronectin), the converse occurred. The former case is consistent with classical random sequential adsorption. The latter result implies constant conformational rearrangement during deposition producing a compact proteinaceous membrane.

Bibliography

Guemouri, L., Ogier, J., & Ramsden, J. J. (1998). Optical properties of protein monolayers during assembly. The Journal of Chemical Physics, 109(8), 3265â3268.

Authors 3

L. Guemouri (first)
J. Ogier (additional)
J. J. Ramsden (additional)

References 32 Referenced 42

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 8:17 a.m.)
Deposited	1 year, 6 months ago (Feb. 11, 2024, 4:10 a.m.)
Indexed	1 day, 21 hours ago (Sept. 3, 2025, 6:20 a.m.)
Issued	27 years ago (Aug. 22, 1998)
Published	27 years ago (Aug. 22, 1998)
Published Print	27 years ago (Aug. 22, 1998)

Funders 0

None

BibTeX

@article{Guemouri_1998, title={Optical properties of protein monolayers during assembly}, volume={109}, ISSN={1089-7690}, url={http://dx.doi.org/10.1063/1.476917}, DOI={10.1063/1.476917}, number={8}, journal={The Journal of Chemical Physics}, publisher={AIP Publishing}, author={Guemouri, L. and Ogier, J. and Ramsden, J. J.}, year={1998}, month=aug, pages={3265–3268} }

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