DOI: 10.1063/1.449781. ‘‘Ordered’’ structure in dilute solutions of biopolymers as studied by small-angle x-ray scattering

‘‘Ordered’’ structure in dilute solutions of biopolymers as studied by small-angle x-ray scattering

10.1063/1.449781

Crossref journal-article

AIP Publishing

The Journal of Chemical Physics (317)

Abstract

Dilute aqueous solutions of bovine serum albumin, lysozyme, chondroitin sulfate, and tRNA were measured by small-angle x-ray scattering. The scattering curves showed a single, broad peak as was observed for synthetic polyelectrolytes, indicating the presence of an ordered distribution of charged solutes. The intermolecular distance evaluated from the peak position (2Dexpt) increased with decreasing polymer concentration and with increasing salt concentration. Except for chondroitin sulfate, 2Dexpt values were nearly equal to the interparticle distance (2D0) calculated based on the assumption of a uniform distribution. The observed relationship between 2Dexpt and 2D0 was in agreement with the proposal that intermacroion attraction is weak for low-charge density particles under discussion. This attraction and repulsive interparticle interaction create a ‘‘secondary’’ minimum in the potential curve enabling ordering to take place. For tRNA, the scattering peak became lower with rising temperature. The fact that only a single, broad peak could be observed was rationalized by invoking the concept of distortions of ordering particles such as the paracrystallinity, and the thermal motion and the crystalline size effect. The correlation hole theory based on repulsive interaction is critically discussed, particularly in light of the experimental fact that the peak position of albumin shifts toward wider angles with increasing number of charges.

Bibliography

Matsuoka, H., Ise, N., Okubo, T., Kunugi, S., Tomiyama, H., & Yoshikawa, Y. (1985). ââOrderedââ structure in dilute solutions of biopolymers as studied by small-angle x-ray scattering. The Journal of Chemical Physics, 83(1), 378â387.

Authors 6

Hideki Matsuoka (first)
Norio Ise (additional)
Tsuneo Okubo (additional)
Shigeru Kunugi (additional)
Hiroshi Tomiyama (additional)
Yukihiro Yoshikawa (additional)

References 49 Referenced 42

{'key': '2024021002054123800_r1'}
10.1021/ar50153a002 / Acc. Chem. Res. (1980)
10.1021/ja00513a068 / J. Am. Chem. Soc. (1979)
10.1002/actp.1979.010300106 / Acta Polym. (1979)
10.1021/ja00547a013 / J. Am. Chem. Soc. (1980)
10.1063/1.444480 / J. Chem. Phys. (1983)
10.1063/1.448009 / J. Chem. Phys. (1984)
10.1039/df9511100107 / Discuss. Faraday Soc. (1951)
10.1085/jgp.25.1.111 / J. Gen. Physiol. (1941)
10.1007/BF01421709 / Colloid Polym. Sci. (1983)
{'key': '2024021002054123800_r11'}
10.1063/1.444479 / J. Chem. Phys. (1983)
10.1021/la00061a033 / Langmuir (1985)
10.1016/0021-9797(73)90224-5 / J. Colloid Interface Sci. (1973)
10.1021/j100727a049 / J. Phys. Chem. (1969)
{'key': '2024021002054123800_r15', 'first-page': '2249', 'volume': '31', 'year': '1982', 'journal-title': 'Polym. Preprints Jpn.'} / Polym. Preprints Jpn. (1982)
10.1080/00268978300102701 / Mol. Phys. (1983)
10.1246/bcsj.41.555 / Bull. Chem. Soc. Jpn. (1968)
10.1111/j.1432-1033.1970.tb01021.x / Eur. J. Biochem. (1970)
{'key': '2024021002054123800_r19', 'first-page': '4187', 'volume': '78', 'year': '1980', 'journal-title': 'J. Chem. Phys.'} / J. Chem. Phys. (1980)
{'key': '2024021002054123800_r20', 'first-page': '78', 'volume': '12', 'year': '1967', 'journal-title': 'Methods Enzymol.'} / Methods Enzymol. (1967)
{'key': '2024021002054123800_r21'}
10.1021/ja01130a017 / J. Am. Chem. Soc. (1952)
{'key': '2024021002054123800_r23'}
10.1021/j100237a030 / J. Phys. Chem. (1983)
10.1021/j100233a001 / J. Phys. Chem. (1983)
10.1021/j150648a003 / J. Phys. Chem. (1984)
10.1063/1.1750183 / J. Chem. Phys. (1938)
10.1021/j100874a036 / J. Phys. Chem. (1966)
10.1021/j100864a016 / J. Phys. Chem. (1967)
10.1021/j100850a053 / J. Phys. Chem. (1968)
10.1063/1.445374 / J. Chem. Phys. (1983)
10.1021/j100879a502 / J. Phys. Chem. (1966)
10.1021/ja00330a005 / J. Am. Chem. Soc. (1984)
{'key': '2024021002054123800_r32'}
10.1063/1.442849 / J. Chem. Phys. (1982)
{'key': '2024021002054123800_r34'}
{'key': '2024021002054123800_r34a'}
{'key': '2024021002054123800_r34b'}
10.1016/0021-9797(85)90102-X / J. Colloid Interface Sci. (1985)
{'key': '2024021002054123800_r36'}
10.1021/ma00246a013 / Macromolecules (1983)
{'key': '2024021002054123800_r38'}
{'key': '2024021002054123800_r39'}
10.1051/jphys:0198200430110167900 / J. Phys. (Paris) (1982)
{'key': '2024021002054123800_r41'}
{'key': '2024021002054123800_r42'}
{'key': '2024021002054123800_r43', 'first-page': '199', 'volume': '96A', 'year': '1983', 'journal-title': 'Phys. Lett.'} / Phys. Lett. (1983)
10.1063/1.447541 / J. Chem. Phys. (1984)

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 9 a.m.)
Deposited	1 year, 6 months ago (Feb. 9, 2024, 10:21 p.m.)
Indexed	1 year, 6 months ago (Feb. 10, 2024, 3:02 p.m.)
Issued	40 years, 2 months ago (July 1, 1985)
Published	40 years, 2 months ago (July 1, 1985)
Published Print	40 years, 2 months ago (July 1, 1985)

Funders 0

None

BibTeX

@article{Matsuoka_1985, title={‘“Ordered”’ structure in dilute solutions of biopolymers as studied by small-angle x-ray scattering}, volume={83}, ISSN={1089-7690}, url={http://dx.doi.org/10.1063/1.449781}, DOI={10.1063/1.449781}, number={1}, journal={The Journal of Chemical Physics}, publisher={AIP Publishing}, author={Matsuoka, Hideki and Ise, Norio and Okubo, Tsuneo and Kunugi, Shigeru and Tomiyama, Hiroshi and Yoshikawa, Yukihiro}, year={1985}, month=jul, pages={378–387} }

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