DOI: 10.1063/1.3008028. Biofunctionalization, cytotoxicity, and cell uptake of lanthanide doped hydrophobically ligated NaYF4 upconversion nanophosphors [Penn]

Biofunctionalization, cytotoxicity, and cell uptake of lanthanide doped hydrophobically ligated NaYF4 upconversion nanophosphors

10.1063/1.3008028

Crossref journal-article

AIP Publishing

Journal of Applied Physics (317)

Abstract

Surface biofunctionalization of the hydrophobic lanthanide ion doped hexagonal phase NaYF4:Yb,Er upconversion nanophosphors (UCNPs) was achieved by introducing amino and carboxyl groups, respectively. Amino groups were added by using the 3-aminopropyltrimethoxysilane reaction after a thin layer of SiO2 coating. The carboxyl groups on surface were added directly by coating modified amphiphilic polyacrylic acid polymer. Experimental studies of cytotoxicity and cell uptake of UCNPs were conducted. The cytotoxicity analysis of the functionalized UCNPs was conducted by methylthiazol tetrazolium assays. Cell uptake was accomplished by incubating the UCNPs with human osteosarcoma cells and proved by transmission electron microscopy. The results showed that the functionalized UCNPs had very low toxicity compared with the control group, while UCNPs taken into the cells indicated that they had very high biocompatibility. The imaging of UCNPs, which were incubated with AB12 mouse mesothelioma cells and excited by 1 W 980 nm light, showed individual particles with visible light emission. These results exhibited promising applications of functionalized UCNPs in cell imaging and photodynamic therapy.

Bibliography

Shan, J., Chen, J., Meng, J., Collins, J., Soboyejo, W., Friedberg, J. S., & Ju, Y. (2008). Biofunctionalization, cytotoxicity, and cell uptake of lanthanide doped hydrophobically ligated NaYF4 upconversion nanophosphors. Journal of Applied Physics, 104(9).

Authors 7

Jingning Shan (first)
Jianbo Chen (additional)
Juan Meng (additional)
Josh Collins (additional)
Wole Soboyejo (additional)
Joseph S. Friedberg (additional)
Yiguang Ju (additional)

References 27 Referenced 116

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Dates

Type	When
Created	16 years, 9 months ago (Nov. 7, 2008, 2:01 p.m.)
Deposited	2 years, 1 month ago (July 6, 2023, 12:17 a.m.)
Indexed	1 month ago (July 30, 2025, 6:51 a.m.)
Issued	16 years, 10 months ago (Nov. 1, 2008)
Published	16 years, 10 months ago (Nov. 1, 2008)
Published Online	16 years, 9 months ago (Nov. 6, 2008)
Published Print	16 years, 10 months ago (Nov. 1, 2008)

Funders 0

None

BibTeX

@article{Shan_2008, title={Biofunctionalization, cytotoxicity, and cell uptake of lanthanide doped hydrophobically ligated NaYF4 upconversion nanophosphors}, volume={104}, ISSN={1089-7550}, url={http://dx.doi.org/10.1063/1.3008028}, DOI={10.1063/1.3008028}, number={9}, journal={Journal of Applied Physics}, publisher={AIP Publishing}, author={Shan, Jingning and Chen, Jianbo and Meng, Juan and Collins, Josh and Soboyejo, Wole and Friedberg, Joseph S. and Ju, Yiguang}, year={2008}, month=nov }

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