DOI: 10.1063/1.1695717. Binding of Mn2+ to Nucleic Acids

10.1063/1.1695717

Crossref journal-article

AIP Publishing

The Journal of Chemical Physics (317)

Abstract

Measurements of the nuclear magnetic resonance relaxation times T1 and T2 of the water protons in aqueous solutions of nucleic acids and magnetic metal ions allow one to determine details about the metal-ion binding. When Mn2+ is bound, the rotation of its hydration sphere becomes slower, so that its effectiveness in reducing the proton T1 from its value in pure water is enhanced. This relaxation enhancement has been measured for Mn2+ bound to DNA, RNA, synthetic polynucleotides, and E. coli ribosomes. Compared to water as unity, the enhancement factors range from 3.7 for polyuridylic acid to 16.7 for polyadenylic acid. The equilibrium constants for binding, and the concentration of binding sites have been calculated from the dependence of enhancement upon Mn2+ concentration.

Bibliography

Eisinger, J., Fawaz-Estrup, F., & Shulman, R. G. (1965). Binding of Mn2+ to Nucleic Acids. The Journal of Chemical Physics, 42(1), 43â53.

Authors 3

J. Eisinger (first)
F. Fawaz-Estrup (additional)
R. G. Shulman (additional)

References 27 Referenced 60

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{'key': '2024020823133093500_r2'}
{'key': '2024020823133093500_r2a'}
10.1016/0006-3002(59)90253-7 / Biochim. Biophys. Acta (1959)
10.1016/0006-3002(61)91012-5 / Biochim. Biophys. Acta (1961)
10.1016/0006-3002(58)90145-8 / Biochim. Biophys. Acta (1958)
10.1063/1.1701258 / J. Chem. Phys. (1962)
{'key': '2024020823133093500_r6'}
10.1021/bi00905a003 / Biochemistry (1963)
10.1016/0926-6550(63)90321-9 / Biochim. Biophys. Acta (1963)
{'key': '2024020823133093500_r9', 'first-page': '415', 'volume': '88', 'year': '1952', 'journal-title': 'Phys. Rev.'} / Phys. Rev. (1952)
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Dates

Type	When
Created	20 years, 7 months ago (Jan. 10, 2005, 10:16 a.m.)
Deposited	1 year, 6 months ago (Feb. 8, 2024, 6:13 p.m.)
Indexed	1 day, 13 hours ago (Sept. 4, 2025, 10:24 a.m.)
Issued	60 years, 8 months ago (Jan. 1, 1965)
Published	60 years, 8 months ago (Jan. 1, 1965)
Published Print	60 years, 8 months ago (Jan. 1, 1965)

Funders 0

None

BibTeX

@article{Eisinger_1965, title={Binding of Mn2+ to Nucleic Acids}, volume={42}, ISSN={1089-7690}, url={http://dx.doi.org/10.1063/1.1695717}, DOI={10.1063/1.1695717}, number={1}, journal={The Journal of Chemical Physics}, publisher={AIP Publishing}, author={Eisinger, J. and Fawaz-Estrup, F. and Shulman, R. G.}, year={1965}, month=jan, pages={43–53} }

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