DOI: 10.1063/1.1332414. Simultaneous dual-color and dual-polarization imaging of single molecules

Simultaneous dual-color and dual-polarization imaging of single molecules

10.1063/1.1332414

Crossref journal-article

AIP Publishing

Applied Physics Letters (317)

Abstract

We report the observation of single-molecule colocalization and quantitative fluorescence resonant energy transfer by simultaneously imaging the emission and polarization characteristics of two colocalized fluorophores using a simple optical design. The methodology was tested using the ligand-receptor system streptavidin, fluorescence labeled with the dye Cy5, and biotin labeled with tetramethylrhodamine. Discrimination of the two dyes permitted the observation of single-pair fluorescence resonant energy transfer with an efficiency of 89%. The multidimensional character of our fluorescence microscopy combined with the robustness of our design provides a simple method suitable to study biomolecular interactions on the single molecule level.

Bibliography

Cognet, L., Harms, G. S., Blab, G. A., Lommerse, P. H. M., & Schmidt, T. (2000). Simultaneous dual-color and dual-polarization imaging of single molecules. Applied Physics Letters, 77(24), 4052â4054.

Authors 5

Laurent Cognet (first)
Gregory S. Harms (additional)
Gerhard A. Blab (additional)
Piet H. M. Lommerse (additional)
Thomas Schmidt (additional)

References 17 Referenced 66

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{'key': '2024020319154397800_r17'}

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 10:14 a.m.)
Deposited	1 year, 6 months ago (Feb. 3, 2024, 2:15 p.m.)
Indexed	1 year ago (Aug. 12, 2024, 9:16 a.m.)
Issued	24 years, 8 months ago (Dec. 11, 2000)
Published	24 years, 8 months ago (Dec. 11, 2000)
Published Print	24 years, 8 months ago (Dec. 11, 2000)

Funders 0

None

BibTeX

@article{Cognet_2000, title={Simultaneous dual-color and dual-polarization imaging of single molecules}, volume={77}, ISSN={1077-3118}, url={http://dx.doi.org/10.1063/1.1332414}, DOI={10.1063/1.1332414}, number={24}, journal={Applied Physics Letters}, publisher={AIP Publishing}, author={Cognet, Laurent and Harms, Gregory S. and Blab, Gerhard A. and Lommerse, Piet H. M. and Schmidt, Thomas}, year={2000}, month=dec, pages={4052–4054} }

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