DOI: 10.1063/1.1145955. Phase contrast studies of biological specimens with the x-ray microscope at BESSY (invited)

Phase contrast studies of biological specimens with the x-ray microscope at BESSY (invited)

10.1063/1.1145955

Crossref journal-article

AIP Publishing

Review of Scientific Instruments (317)

Abstract

The transmission x-ray microscope at the BESSY storage ring is being used for investigations in the fields of biology, biophysics, medicine, colloid chemistry, and soil sciences. The system has been instrumented for phase contrast studies with the possibility of quick change between imaging in amplitude and phase contrast mode. Phase contrast has the advantage of considerable higher image contrast and reduced radiation dosage compared to amplitude contrast. A cryogenic object chamber has been implemented on the x-ray microscope. First experiments have shown that at cryogenic temperatures the structural stability of biological specimens is increased by three orders of magnitude in comparison with unfixed wet specimens at room temperature.

Bibliography

Schmahl, G., Rudolph, D., Guttmann, P., Schneider, G., Thieme, J., & Niemann, B. (1995). Phase contrast studies of biological specimens with the x-ray microscope at BESSY (invited). Review of Scientific Instruments, 66(2), 1282â1286.

Authors 6

Günter Schmahl (first)
Dietbert Rudolph (additional)
Peter Guttmann (additional)
Gerd Schneider (additional)
Jürgen Thieme (additional)
Bastian Niemann (additional)

References 23 Referenced 112

{'key': '2023070816532278500_r1'}
10.1364/AO.15.001883 / Appl. Opt. (1976)
{'key': '2023070816532278500_r3', 'first-page': '297', 'volume': '133', 'year': '1980', 'journal-title': 'Quart. Rev. Biophys.'} / Quart. Rev. Biophys. (1980)
10.1117/12.964940 / Proc. SPIE (1986)
{'key': '2023070816532278500_r5'}
{'key': '2023070816532278500_r6'}
{'key': '2023070816532278500_r7'}
{'key': '2023070816532278500_r8'}
{'key': '2023070816532278500_r9'}
{'key': '2023070816532278500_r10', 'first-page': '305', 'volume': '33a', 'year': '1978', 'journal-title': 'Z. Naturforschung'} / Z. Naturforschung (1978)
{'key': '2023070816532278500_r11'}
{'key': '2023070816532278500_r12'}
{'key': '2023070816532278500_r13', 'first-page': '454', 'volume': '16', 'year': '1935', 'journal-title': 'Z. Tech. Phys.'} / Z. Tech. Phys. (1935)
{'key': '2023070816532278500_r14'}
{'key': '2023070816532278500_r15'}
{'key': '2023070816532278500_r16'}
{'key': '2023070816532278500_r17'}
{'key': '2023070816532278500_r18'}
{'key': '2023070816532278500_r19'}
{'key': '2023070816532278500_r20'}
{'key': '2023070816532278500_r21'}
{'key': '2023070816532278500_r22'}
{'key': '2023070816532278500_r23'}

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 8:03 a.m.)
Deposited	2 years, 1 month ago (July 8, 2023, 12:53 p.m.)
Indexed	1 month, 1 week ago (July 14, 2025, 11:34 p.m.)
Issued	30 years, 6 months ago (Feb. 1, 1995)
Published	30 years, 6 months ago (Feb. 1, 1995)
Published Print	30 years, 6 months ago (Feb. 1, 1995)

Funders 0

None

BibTeX

@article{Schmahl_1995, title={Phase contrast studies of biological specimens with the x-ray microscope at BESSY (invited)}, volume={66}, ISSN={1089-7623}, url={http://dx.doi.org/10.1063/1.1145955}, DOI={10.1063/1.1145955}, number={2}, journal={Review of Scientific Instruments}, publisher={AIP Publishing}, author={Schmahl, Günter and Rudolph, Dietbert and Guttmann, Peter and Schneider, Gerd and Thieme, Jürgen and Niemann, Bastian}, year={1995}, month=feb, pages={1282–1286} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        15
      ]
    ],
    "date-time": "2025-07-15T03:34:42Z",
    "timestamp": 1752550482842
  },
  "reference-count": 23,
  "publisher": "AIP Publishing",
  "issue": "2",
  "content-domain": {
    "domain": [
      "pubs.aip.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        2,
        1
      ]
    ]
  },
  "abstract": "<jats:p>The transmission x-ray microscope at the BESSY storage ring is being used for investigations in the fields of biology, biophysics, medicine, colloid chemistry, and soil sciences. The system has been instrumented for phase contrast studies with the possibility of quick change between imaging in amplitude and phase contrast mode. Phase contrast has the advantage of considerable higher image contrast and reduced radiation dosage compared to amplitude contrast. A cryogenic object chamber has been implemented on the x-ray microscope. First experiments have shown that at cryogenic temperatures the structural stability of biological specimens is increased by three orders of magnitude in comparison with unfixed wet specimens at room temperature.</jats:p>",
  "DOI": "10.1063/1.1145955",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        26
      ]
    ],
    "date-time": "2002-07-26T12:03:22Z",
    "timestamp": 1027685002000
  },
  "page": "1282-1286",
  "update-policy": "http://dx.doi.org/10.1063/aip-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 112,
  "title": "Phase contrast studies of biological specimens with the x-ray microscope at BESSY (invited)",
  "prefix": "10.1063",
  "volume": "66",
  "author": [
    {
      "given": "G\u00fcnter",
      "family": "Schmahl",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Forschungseinrichtung R\u00f6ntgenphysik, Georg-August-Universit\u00e4t G\u00f6ttingen, Geiststrasse 11, D-37073 G\u00f6ttingen, Germany"
        }
      ]
    },
    {
      "given": "Dietbert",
      "family": "Rudolph",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Forschungseinrichtung R\u00f6ntgenphysik, Georg-August-Universit\u00e4t G\u00f6ttingen, Geiststrasse 11, D-37073 G\u00f6ttingen, Germany"
        }
      ]
    },
    {
      "given": "Peter",
      "family": "Guttmann",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Forschungseinrichtung R\u00f6ntgenphysik, Georg-August-Universit\u00e4t G\u00f6ttingen, Geiststrasse 11, D-37073 G\u00f6ttingen, Germany"
        }
      ]
    },
    {
      "given": "Gerd",
      "family": "Schneider",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Forschungseinrichtung R\u00f6ntgenphysik, Georg-August-Universit\u00e4t G\u00f6ttingen, Geiststrasse 11, D-37073 G\u00f6ttingen, Germany"
        }
      ]
    },
    {
      "given": "J\u00fcrgen",
      "family": "Thieme",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Forschungseinrichtung R\u00f6ntgenphysik, Georg-August-Universit\u00e4t G\u00f6ttingen, Geiststrasse 11, D-37073 G\u00f6ttingen, Germany"
        }
      ]
    },
    {
      "given": "Bastian",
      "family": "Niemann",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Forschungseinrichtung R\u00f6ntgenphysik, Georg-August-Universit\u00e4t G\u00f6ttingen, Geiststrasse 11, D-37073 G\u00f6ttingen, Germany"
        }
      ]
    }
  ],
  "member": "317",
  "reference": [
    {
      "key": "2023070816532278500_r1"
    },
    {
      "key": "2023070816532278500_r2",
      "doi-asserted-by": "crossref",
      "first-page": "1883",
      "DOI": "10.1364/AO.15.001883",
      "volume": "15",
      "year": "1976",
      "journal-title": "Appl. Opt."
    },
    {
      "key": "2023070816532278500_r3",
      "first-page": "297",
      "volume": "133",
      "year": "1980",
      "journal-title": "Quart. Rev. Biophys."
    },
    {
      "key": "2023070816532278500_r4",
      "doi-asserted-by": "crossref",
      "first-page": "422",
      "DOI": "10.1117/12.964940",
      "volume": "733",
      "year": "1986",
      "journal-title": "Proc. SPIE"
    },
    {
      "key": "2023070816532278500_r5"
    },
    {
      "key": "2023070816532278500_r6"
    },
    {
      "key": "2023070816532278500_r7"
    },
    {
      "key": "2023070816532278500_r8"
    },
    {
      "key": "2023070816532278500_r9"
    },
    {
      "key": "2023070816532278500_r10",
      "first-page": "305",
      "volume": "33a",
      "year": "1978",
      "journal-title": "Z. Naturforschung"
    },
    {
      "key": "2023070816532278500_r11"
    },
    {
      "key": "2023070816532278500_r12"
    },
    {
      "key": "2023070816532278500_r13",
      "first-page": "454",
      "volume": "16",
      "year": "1935",
      "journal-title": "Z. Tech. Phys."
    },
    {
      "key": "2023070816532278500_r14"
    },
    {
      "key": "2023070816532278500_r15"
    },
    {
      "key": "2023070816532278500_r16"
    },
    {
      "key": "2023070816532278500_r17"
    },
    {
      "key": "2023070816532278500_r18"
    },
    {
      "key": "2023070816532278500_r19"
    },
    {
      "key": "2023070816532278500_r20"
    },
    {
      "key": "2023070816532278500_r21"
    },
    {
      "key": "2023070816532278500_r22"
    },
    {
      "key": "2023070816532278500_r23"
    }
  ],
  "container-title": "Review of Scientific Instruments",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pubs.aip.org/aip/rsi/article-pdf/66/2/1282/8808369/1282_1_online.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://pubs.aip.org/aip/rsi/article-pdf/66/2/1282/8808369/1282_1_online.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        7,
        8
      ]
    ],
    "date-time": "2023-07-08T16:53:34Z",
    "timestamp": 1688835214000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pubs.aip.org/rsi/article/66/2/1282/459486/Phase-contrast-studies-of-biological-specimens"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        2,
        1
      ]
    ]
  },
  "references-count": 23,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1995,
          2,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1063/1.1145955",
  "relation": {},
  "ISSN": [
    "0034-6748",
    "1089-7623"
  ],
  "subject": [],
  "published-other": {
    "date-parts": [
      [
        1995,
        2
      ]
    ]
  },
  "published": {
    "date-parts": [
      [
        1995,
        2,
        1
      ]
    ]
  }
}