DOI: 10.1046/j.1524-475x.1999.00511.x. Expression of apoptosis‐associated genes by human dermal scar fibroblasts

Expression of apoptosis‐associated genes by human dermal scar fibroblasts

10.1046/j.1524-475x.1999.00511.x

Crossref journal-article

Wiley

Wound Repair and Regeneration (311)

Abstract

The purpose of this study was to determine if aberrant apoptosis plays a role in pathologic wound healing as manifested by hypertrophic scarring and keloid formation. Apoptosis has recently been found to participate in the transition between granulation tissue and the development of definitive scar. The question that remains to be answered is what stimuli initiate apoptosis during wound healing. Hitherto, regulatory factors and pathways involved have been largely undefined. We investigated heterogeneity among fibroblasts derived from normal skin and keloid scar, by examining apoptotic profiles and pathways for these cells. Quantitative analysis of apoptotic cells using an Annexin‐V‐FITC binding assay showed that normal skin fibroblast cultures were found to have a two‐fold higher percentage of apoptotic cells than did keloid fibroblast cultures. To study apoptotic pathways and related death‐associated genes, a ribonuclease protection assay was performed for fibroblasts exposed to anti‐Fas antibody and tumor necrosis factor‐α to activate the Fas/TNF receptor apoptotic pathway. Compared with normal skin fibroblasts, keloid fibroblasts exhibited decreased expression of apoptosis‐associated genes.

Bibliography

Messadi, D. V., Le, A., Berg, S., Jewett, A., Wen, Z., Kelly, P., & Bertolami, C. N. (1999). Expression of apoptosisâassociated genes by human dermal scar fibroblasts. Wound Repair and Regeneration, 7(6), 511â517. Portico.

Authors 7

Diana V. Messadi (first)
Anh Le (additional)
Steve Berg (additional)
Anahid Jewett (additional)
Zhuang Wen (additional)
Paul Kelly (additional)
Charles N. Bertolami (additional)

References 34 Referenced 66

10.1002/path.1711050103
10.1038/sj.leu.2400626
10.1126/science.7878464
10.1016/S1357-2725(96)00117-3
{'key': 'e_1_2_7_6_2', 'first-page': '56', 'article-title': 'Apoptosis mediates the decrease in cellularity during the transition between granulation tissue and scar', 'volume': '146', 'author': 'Desmouliere A', 'year': '1995', 'journal-title': 'Am J Pathol'} / Am J Pathol / Apoptosis mediates the decrease in cellularity during the transition between granulation tissue and scar by Desmouliere A (1995)
10.1016/S1357-4310(97)80541-2
{'key': 'e_1_2_7_8_2', 'first-page': '1699', 'article-title': 'Hypertrophic scars and keloids: a review and new concept concerning their origin', 'volume': '4', 'author': 'Kischer CW', 'year': '1982', 'journal-title': 'Scan Elect Microsc'} / Scan Elect Microsc / Hypertrophic scars and keloids: a review and new concept concerning their origin by Kischer CW (1982)
{'key': 'e_1_2_7_9_2', 'first-page': '1441', 'article-title': 'Short communication. Apoptosis, neurosis, and proliferation. possible implications in the etiology of keloids', 'volume': '149', 'author': 'Appleton I', 'year': '1996', 'journal-title': 'Am J Pathol'} / Am J Pathol / Short communication. Apoptosis, neurosis, and proliferation. possible implications in the etiology of keloids by Appleton I (1996)
10.1146/annurev.med.48.1.267
10.1016/0092-8674(93)90464-2
10.1016/0092-8674(94)90372-7
10.1111/1523-1747.ep12363594
10.1111/1523-1747.ep12334273
10.1046/j.1524-475X.1998.60106.x
{'key': 'e_1_2_7_16_2', 'first-page': '31', 'article-title': 'Role of apoptosis in keloid formation', 'volume': '23', 'author': 'Messadi DV', 'year': '1998', 'journal-title': 'Int J Oral Biol'} / Int J Oral Biol / Role of apoptosis in keloid formation by Messadi DV (1998)
{'key': 'e_1_2_7_17_2', 'first-page': '1041', 'article-title': 'CD44 and hyaluronan expression in human cutaneous scar fibroblasts', 'volume': '142', 'author': 'Messadi DV', 'year': '1993', 'journal-title': 'Am J Pathol'} / Am J Pathol / CD44 and hyaluronan expression in human cutaneous scar fibroblasts by Messadi DV (1993)
10.1016/0022-1759(95)00072-I
10.1016/0003-2697(87)90021-2
{'key': 'e_1_2_7_20_2', 'first-page': '471', 'volume-title': 'Current protocols in molecular biology', 'author': 'Gilman M', 'year': '1993'} / Current protocols in molecular biology by Gilman M (1993)
10.1084/jem.183.4.1339
10.1006/jsre.1996.0127
10.2340/0001555575102104 / Acta Derm Venereol (Stockh) / Proliferation activity of dermal fibroblasts in keloids and hypertrophic scars by Nakakao H (1995)
{'key': 'e_1_2_7_24_2', 'first-page': '1639', 'article-title': 'Induction of fibroblast apoptosis by anti‐CD44 antibody. Implications for the treatment of fibroproliferative lung disease', 'volume': '149', 'author': 'Henke C', 'year': '1996', 'journal-title': 'Am J Pathol'} / Am J Pathol / Induction of fibroblast apoptosis by anti‐CD44 antibody. Implications for the treatment of fibroproliferative lung disease by Henke C (1996)
10.1016/0962-8924(93)90128-N
10.1016/0968-0004(94)90170-8
10.1016/0092-8674(95)90071-3
10.1016/0092-8674(95)90070-5
10.1016/0092-8674(95)90072-1
10.1074/jbc.270.1.387
{'key': 'e_1_2_7_31_2', 'first-page': '17255', 'article-title': 'I‐FLICE, a novel inhibitor of tumor necrosis factor receptor‐1‐ and CD‐95‐induced apoptosis', 'volume': '28', 'author': 'Hu S', 'year': '1995', 'journal-title': 'J Biol Chem'} / J Biol Chem / I‐FLICE, a novel inhibitor of tumor necrosis factor receptor‐1‐ and CD‐95‐induced apoptosis by Hu S (1995)
10.1016/S0092-8674(00)81266-0
10.1016/0092-8674(95)90422-0
10.1038/376037a0
10.1126/science.274.5288.782

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:53 p.m.)
Deposited	1 year, 11 months ago (Sept. 11, 2023, 12:53 a.m.)
Indexed	11 months ago (Oct. 5, 2024, 8:28 p.m.)
Issued	25 years, 10 months ago (Nov. 1, 1999)
Published	25 years, 10 months ago (Nov. 1, 1999)
Published Online	23 years, 8 months ago (Jan. 5, 2002)
Published Print	25 years, 10 months ago (Nov. 1, 1999)

Funders 0

None

BibTeX

@article{Messadi_1999, title={Expression of apoptosis‐associated genes by human dermal scar fibroblasts}, volume={7}, ISSN={1524-475X}, url={http://dx.doi.org/10.1046/j.1524-475x.1999.00511.x}, DOI={10.1046/j.1524-475x.1999.00511.x}, number={6}, journal={Wound Repair and Regeneration}, publisher={Wiley}, author={Messadi, Diana V. and Le, Anh and Berg, Steve and Jewett, Anahid and Wen, Zhuang and Kelly, Paul and Bertolami, Charles N.}, year={1999}, month=nov, pages={511–517} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        10,
        6
      ]
    ],
    "date-time": "2024-10-06T00:28:49Z",
    "timestamp": 1728174529111
  },
  "reference-count": 34,
  "publisher": "Wiley",
  "issue": "6",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2002,
            1,
            5
          ]
        ],
        "date-time": "2002-01-05T00:00:00Z",
        "timestamp": 1010188800000
      },
      "content-version": "vor",
      "delay-in-days": 796,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1999,
        11
      ]
    ]
  },
  "abstract": "<jats:p>The purpose of this study was to determine if aberrant apoptosis plays a role in pathologic wound healing as manifested by hypertrophic scarring and keloid formation. Apoptosis has recently been found to participate in the transition between granulation tissue and the development of definitive scar. The question that remains to be answered is what stimuli initiate apoptosis during wound healing. Hitherto, regulatory factors and pathways involved have been largely undefined. We investigated heterogeneity among fibroblasts derived from normal skin and keloid scar, by examining apoptotic profiles and pathways for these cells. Quantitative analysis of apoptotic cells using an Annexin\u2010V\u2010FITC binding assay showed that normal skin fibroblast cultures were found to have a two\u2010fold higher percentage of apoptotic cells than did keloid fibroblast cultures. To study apoptotic pathways and related death\u2010associated genes, a ribonuclease protection assay was performed for fibroblasts exposed to anti\u2010Fas antibody and tumor necrosis factor\u2010\u03b1 to activate the Fas/TNF receptor apoptotic pathway. Compared with normal skin fibroblasts, keloid fibroblasts exhibited decreased expression of apoptosis\u2010associated genes.</jats:p>",
  "DOI": "10.1046/j.1524-475x.1999.00511.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        3,
        12
      ]
    ],
    "date-time": "2003-03-12T23:53:11Z",
    "timestamp": 1047513191000
  },
  "page": "511-517",
  "source": "Crossref",
  "is-referenced-by-count": 66,
  "title": "Expression of apoptosis\u2010associated genes by human dermal scar fibroblasts",
  "prefix": "10.1111",
  "volume": "7",
  "author": [
    {
      "given": "Diana V.",
      "family": "Messadi",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Anh",
      "family": "Le",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Steve",
      "family": "Berg",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Anahid",
      "family": "Jewett",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Zhuang",
      "family": "Wen",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Paul",
      "family": "Kelly",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Charles N.",
      "family": "Bertolami",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2002,
        1,
        5
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_7_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/path.1711050103"
    },
    {
      "key": "e_1_2_7_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/sj.leu.2400626"
    },
    {
      "key": "e_1_2_7_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.7878464"
    },
    {
      "key": "e_1_2_7_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1357-2725(96)00117-3"
    },
    {
      "key": "e_1_2_7_6_2",
      "first-page": "56",
      "article-title": "Apoptosis mediates the decrease in cellularity during the transition between granulation tissue and scar",
      "volume": "146",
      "author": "Desmouliere A",
      "year": "1995",
      "journal-title": "Am J Pathol"
    },
    {
      "key": "e_1_2_7_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1357-4310(97)80541-2"
    },
    {
      "key": "e_1_2_7_8_2",
      "first-page": "1699",
      "article-title": "Hypertrophic scars and keloids: a review and new concept concerning their origin",
      "volume": "4",
      "author": "Kischer CW",
      "year": "1982",
      "journal-title": "Scan Elect Microsc"
    },
    {
      "key": "e_1_2_7_9_2",
      "first-page": "1441",
      "article-title": "Short communication. Apoptosis, neurosis, and proliferation. possible implications in the etiology of keloids",
      "volume": "149",
      "author": "Appleton I",
      "year": "1996",
      "journal-title": "Am J Pathol"
    },
    {
      "key": "e_1_2_7_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.med.48.1.267"
    },
    {
      "key": "e_1_2_7_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(93)90464-2"
    },
    {
      "key": "e_1_2_7_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(94)90372-7"
    },
    {
      "key": "e_1_2_7_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/1523-1747.ep12363594"
    },
    {
      "key": "e_1_2_7_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/1523-1747.ep12334273"
    },
    {
      "key": "e_1_2_7_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1524-475X.1998.60106.x"
    },
    {
      "key": "e_1_2_7_16_2",
      "first-page": "31",
      "article-title": "Role of apoptosis in keloid formation",
      "volume": "23",
      "author": "Messadi DV",
      "year": "1998",
      "journal-title": "Int J Oral Biol"
    },
    {
      "key": "e_1_2_7_17_2",
      "first-page": "1041",
      "article-title": "CD44 and hyaluronan expression in human cutaneous scar fibroblasts",
      "volume": "142",
      "author": "Messadi DV",
      "year": "1993",
      "journal-title": "Am J Pathol"
    },
    {
      "key": "e_1_2_7_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-1759(95)00072-I"
    },
    {
      "key": "e_1_2_7_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-2697(87)90021-2"
    },
    {
      "key": "e_1_2_7_20_2",
      "first-page": "471",
      "volume-title": "Current protocols in molecular biology",
      "author": "Gilman M",
      "year": "1993"
    },
    {
      "key": "e_1_2_7_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1084/jem.183.4.1339"
    },
    {
      "key": "e_1_2_7_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/jsre.1996.0127"
    },
    {
      "key": "e_1_2_7_23_2",
      "doi-asserted-by": "crossref",
      "first-page": "102",
      "DOI": "10.2340/0001555575102104",
      "article-title": "Proliferation activity of dermal fibroblasts in keloids and hypertrophic scars",
      "volume": "75",
      "author": "Nakakao H",
      "year": "1995",
      "journal-title": "Acta Derm Venereol (Stockh)"
    },
    {
      "key": "e_1_2_7_24_2",
      "first-page": "1639",
      "article-title": "Induction of fibroblast apoptosis by anti\u2010CD44 antibody. Implications for the treatment of fibroproliferative lung disease",
      "volume": "149",
      "author": "Henke C",
      "year": "1996",
      "journal-title": "Am J Pathol"
    },
    {
      "key": "e_1_2_7_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0962-8924(93)90128-N"
    },
    {
      "key": "e_1_2_7_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0968-0004(94)90170-8"
    },
    {
      "key": "e_1_2_7_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90071-3"
    },
    {
      "key": "e_1_2_7_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90070-5"
    },
    {
      "key": "e_1_2_7_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90072-1"
    },
    {
      "key": "e_1_2_7_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.270.1.387"
    },
    {
      "key": "e_1_2_7_31_2",
      "first-page": "17255",
      "article-title": "I\u2010FLICE, a novel inhibitor of tumor necrosis factor receptor\u20101\u2010 and CD\u201095\u2010induced apoptosis",
      "volume": "28",
      "author": "Hu S",
      "year": "1995",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "e_1_2_7_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81266-0"
    },
    {
      "key": "e_1_2_7_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90422-0"
    },
    {
      "key": "e_1_2_7_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/376037a0"
    },
    {
      "key": "e_1_2_7_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.274.5288.782"
    }
  ],
  "container-title": "Wound Repair and Regeneration",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1524-475X.1999.00511.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1524-475X.1999.00511.x",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/full-xml/10.1046/j.1524-475X.1999.00511.x",
      "content-type": "application/xml",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1524-475X.1999.00511.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        9,
        11
      ]
    ],
    "date-time": "2023-09-11T04:53:16Z",
    "timestamp": 1694407996000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1046/j.1524-475X.1999.00511.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1999,
        11
      ]
    ]
  },
  "references-count": 34,
  "journal-issue": {
    "issue": "6",
    "published-print": {
      "date-parts": [
        [
          1999,
          11
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1046/j.1524-475X.1999.00511.x"
  ],
  "URL": "http://dx.doi.org/10.1046/j.1524-475X.1999.00511.x",
  "relation": {},
  "ISSN": [
    "1067-1927",
    "1524-475X"
  ],
  "subject": [],
  "container-title-short": "Wound Repair Regeneration",
  "published": {
    "date-parts": [
      [
        1999,
        11
      ]
    ]
  }
}