DOI: 10.1046/j.1471-4159.2002.01260.x. Microarray analysis of selected genes in neural stem and progenitor cells

Microarray analysis of selected genes in neural stem and progenitor cells

10.1046/j.1471-4159.2002.01260.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

AbstractTo access and compare gene expression in fetal neuroepithelial cells (NEPs) and progenitor cells, we have used microarrays containing approximately 500 known genes related to cell cycle regulation, apoptosis, growth and differentiation. We have identified 152 genes that are expressed in NEPs and 209 genes expressed by progenitor cells. The majority of genes (141) detected in NEPs are also present in progenitor populations. There are 68 genes specifically expressed in progenitors with little or no expression in NEPs, and a few genes that appear to be present exclusively in NEPs. Using cell sorting, RT–PCR, in situ hybridization or immunocytochemistry, we have examined the segregation of expression to neuronal and glial progenitors, and identified several that appeared to be enriched in neuronal (e.g. CDK5, neuropilin, EphrinB2, FGF11) or glial (e.g. CXCR4, RhoC, CD44, tenascin C) precursors. Our data provide a first report of gene expression profiles of neural stem and progenitor cells at early stages of development, and provide evidence for the potential roles of specific cell cycle regulators, chemokines, cytokines and extracellular matrix molecules in neural development and lineage segregation.

Bibliography

Luo, Y., Cai, J., Liu, Y., Xue, H., Chrest, F. J., Wersto, R. P., & Rao, M. (2002). Microarray analysis of selected genes in neural stem and progenitor cells. Journal of Neurochemistry, 83(6), 1481â1497. Portico.

Authors 7

Yongquan Luo (first)
Jingli Cai (additional)
Ying Liu (additional)
Haipeng Xue (additional)
Francis J. Chrest (additional)
Robert P. Wersto (additional)
Mahendra Rao (additional)

References 61 Referenced 77

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 2:03 a.m.)
Deposited	1 year, 10 months ago (Oct. 17, 2023, 1:29 p.m.)
Indexed	1 month, 3 weeks ago (July 7, 2025, 1:45 a.m.)
Issued	22 years, 8 months ago (Dec. 1, 2002)
Published	22 years, 8 months ago (Dec. 1, 2002)
Published Online	22 years, 8 months ago (Dec. 11, 2002)
Published Print	22 years, 8 months ago (Dec. 1, 2002)

Funders 0

None

BibTeX

@article{Luo_2002, title={Microarray analysis of selected genes in neural stem and progenitor cells}, volume={83}, ISSN={1471-4159}, url={http://dx.doi.org/10.1046/j.1471-4159.2002.01260.x}, DOI={10.1046/j.1471-4159.2002.01260.x}, number={6}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Luo, Yongquan and Cai, Jingli and Liu, Ying and Xue, Haipeng and Chrest, Francis J. and Wersto, Robert P. and Rao, Mahendra}, year={2002}, month=dec, pages={1481–1497} }

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    "10.1046/j.1471-4159.2002.01260.x"
  ],
  "URL": "http://dx.doi.org/10.1046/j.1471-4159.2002.01260.x",
  "relation": {},
  "ISSN": [
    "0022-3042",
    "1471-4159"
  ],
  "subject": [],
  "container-title-short": "Journal of Neurochemistry",
  "published": {
    "date-parts": [
      [
        2002,
        12
      ]
    ]
  }
}