DOI: 10.1046/j.1471-4159.2002.01137.x. Proteasomal degradation of tau protein

10.1046/j.1471-4159.2002.01137.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

AbstractFilamentous inclusions composed of the microtubule‐associated protein tau are a defining characteristic of a large number of neurodegenerative diseases. Here we show that tau degradation in stably transfected and non‐transfected SH‐SY5Y cells is blocked by the irreversible proteasome inhibitor lactacystin. Further, we find that in vitro, natively unfolded tau can be directly processed by the 20S proteasome without a requirement for ubiquitylation, and that a highly reproducible pattern of degradation intermediates is readily detectable during this process. Analysis of these intermediates shows that 20S proteasomal processing of tau is bi‐directional, proceeding from both N‐ and C‐termini, and that populations of relatively stable intermediates arise probably because of less efficient digestion of the C‐terminal repeat region. Our results are consistent with an in vivo role for the proteasome in tau degradation and support the existence of ubiquitin‐independent pathways for the proteasomal degradation of unfolded proteins.

Bibliography

David, D. C., Layfield, R., Serpell, L., Narain, Y., Goedert, M., & Spillantini, M. G. (2002). Proteasomal degradation of tau protein. Journal of Neurochemistry, 83(1), 176â185. Portico.

Authors 6

Della C. David (first)
Robert Layfield (additional)
Louise Serpell (additional)
Yolanda Narain (additional)
Michel Goedert (additional)
Maria Grazia Spillantini (additional)

References 56 Referenced 258

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 2:02 a.m.)
Deposited	1 year, 10 months ago (Oct. 17, 2023, 12:20 p.m.)
Indexed	1 month ago (July 24, 2025, 6:54 a.m.)
Issued	22 years, 11 months ago (Sept. 18, 2002)
Published	22 years, 11 months ago (Sept. 18, 2002)
Published Online	22 years, 11 months ago (Sept. 18, 2002)
Published Print	22 years, 10 months ago (Oct. 1, 2002)

Funders 0

None

BibTeX

@article{David_2002, title={Proteasomal degradation of tau protein}, volume={83}, ISSN={1471-4159}, url={http://dx.doi.org/10.1046/j.1471-4159.2002.01137.x}, DOI={10.1046/j.1471-4159.2002.01137.x}, number={1}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={David, Della C. and Layfield, Robert and Serpell, Louise and Narain, Yolanda and Goedert, Michel and Spillantini, Maria Grazia}, year={2002}, month=sep, pages={176–185} }

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