PI 3‐kinase regulation of dopamine uptake
10.1046/j.1471-4159.2002.00892.x
Crossref journal-article
Wiley
Journal of Neurochemistry (311)
Abstract

AbstractThe magnitude and duration of dopamine (DA) signaling is defined by the amount of vesicular release, DA receptor sensitivity, and the efficiency of DA clearance, which is largely determined by the DA transporter (DAT). DAT uptake capacity is determined by the number of functional transporters on the cell surface as well as by their turnover rate. Here we show that inhibition of phosphatidylinositol (PI) 3‐kinase with LY294002 induces internalization of the human DAT (hDAT), thereby reducing transport capacity. Acute treatment with LY294002 reduced the maximal rate of [3H]DA uptake in rat striatal synaptosomes and in human embryonic kidney (HEK) 293 cells stably expressing the hDAT (hDAT cells). In addition, LY294002 caused a significant redistribution of the hDAT from the plasma membrane to the cytosol. Conversely, insulin, which activates PI 3‐kinase, increased [3H]DA uptake and blocked the amphetamine‐induced hDAT intracellular accumulation, as did transient expression of constitutively active PI 3‐kinase. The LY294002‐induced reduction in [3H]DA uptake and hDAT cell surface expression was inhibited by expression of a dominant negative mutant of dynamin I, indicating that dynamin‐dependent trafficking can modulate transport capacity. These data implicate DAT trafficking in the hormonal regulation of dopaminergic signaling, and suggest that a state of chronic hypoinsulinemia, such as in diabetes, may alter synaptic DA signaling by reducing the available cell surface DATs.

Bibliography

Carvelli, L., Morón, J. A., Kahlig, K. M., Ferrer, J. V., Sen, N., Lechleiter, J. D., Leeb‐Lundberg, L. M. F., Merrill, G., Lafer, E. M., Ballou, L. M., Shippenberg, T. S., Javitch, J. A., Lin, R. Z., & Galli, A. (2002). PI 3‐kinase regulation of dopamine uptake. Journal of Neurochemistry, 81(4), 859–869. Portico.

Authors 14
  1. Lucia Carvelli (first)
  2. José A. Morón (additional)
  3. Kristopher M. Kahlig (additional)
  4. Jasmine V. Ferrer (additional)
  5. Namita Sen (additional)
  6. James D. Lechleiter (additional)
  7. L. M. Fredrik Leeb‐Lundberg (additional)
  8. Gerald Merrill (additional)
  9. Eileen M. Lafer (additional)
  10. Lisa M. Ballou (additional)
  11. Toni S. Shippenberg (additional)
  12. Jonathan A. Javitch (additional)
  13. Richard Z. Lin (additional)
  14. Aurelio Galli (additional)
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Dates
Type When
Created 22 years, 5 months ago (March 12, 2003, 2 a.m.)
Deposited 1 year, 11 months ago (Sept. 27, 2023, 9 p.m.)
Indexed 4 days, 19 hours ago (Aug. 24, 2025, 7 p.m.)
Issued 23 years, 3 months ago (May 7, 2002)
Published 23 years, 3 months ago (May 7, 2002)
Published Online 23 years, 3 months ago (May 7, 2002)
Published Print 23 years, 3 months ago (May 15, 2002)
Funders 0

None

@article{Carvelli_2002, title={PI 3‐kinase regulation of dopamine uptake}, volume={81}, ISSN={1471-4159}, url={http://dx.doi.org/10.1046/j.1471-4159.2002.00892.x}, DOI={10.1046/j.1471-4159.2002.00892.x}, number={4}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Carvelli, Lucia and Morón, José A. and Kahlig, Kristopher M. and Ferrer, Jasmine V. and Sen, Namita and Lechleiter, James D. and Leeb‐Lundberg, L. M. Fredrik and Merrill, Gerald and Lafer, Eileen M. and Ballou, Lisa M. and Shippenberg, Toni S. and Javitch, Jonathan A. and Lin, Richard Z. and Galli, Aurelio}, year={2002}, month=may, pages={859–869} }