DOI: 10.1046/j.1471-4159.1999.0720962.x. Dynamic Interaction Between Soluble Tubulin and C‐Terminal Domains of <i>N</i>‐Methyl‐D‐Aspartate Receptor Subu

Dynamic Interaction Between Soluble Tubulin and C‐Terminal Domains of N‐Methyl‐D‐Aspartate Receptor Subu

10.1046/j.1471-4159.1999.0720962.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract : The cytoplasmic C‐terminal domains (CTs) of the NR1 and NR2 subunits of the NMDA receptor have been implicated in its anchoring to the subsynaptic cytoskeleton. Here, we used affinity chromatography with glutathione S‐transferase‐NR1‐CT and ‐NR2B‐CT fusion proteins to identify novel binding partner(s) of these NMDA receptor subunits. Upon incubation with rat brain cytosolic protein fraction, both NR1‐CT and NR2B‐CT, but not glutathione S‐transferase, specifically bound tubulin. The respective fusion proteins also bound tubulin purified from brain, suggesting a direct interaction between the two binding partners. In tubulin polymerization assays, NR1‐CT and NR2B‐CT significantly decreased the rate of microtubule formation without destabilizing preformed microtubules. Moreover, only minor fractions of either fusion protein coprecipitated with the newly formed microtubules. Consistent with these findings, ultrastructural analysis of the newly formed microtubules revealed a limited association only with the CTs of the NR1 and NR2B. These data suggest a direct interaction of the NMDA receptor channel subunit CTs and tubulin dimers or soluble forms of tubulin. The efficient modulation of microtubule dynamics by the NR1 and NR2 cytoplasmic domains suggests a functional interaction of the receptor and the subsynaptic cytoskeletal network that may play a role during morphological adaptations, as observed during synaptogenesis and in adult CNS plasticity.

Bibliography

Van Rossum, D., Kuhse, J., & Betz, H. (1999). Dynamic Interaction Between Soluble Tubulin and CâTerminal Domains of NâMethylâDâAspartate Receptor Subu. Journal of Neurochemistry, 72(3), 962â973. Portico.

Authors 3

Denise Van Rossum (first)
Jochen Kuhse (additional)
Heinrich Betz (additional)

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 1:36 a.m.)
Deposited	1 year, 10 months ago (Oct. 28, 2023, 8:14 p.m.)
Indexed	1 year, 2 months ago (June 20, 2024, 6:35 p.m.)
Issued	26 years, 6 months ago (March 1, 1999)
Published	26 years, 6 months ago (March 1, 1999)
Published Online	17 years, 2 months ago (July 7, 2008)
Published Print	26 years, 6 months ago (March 1, 1999)

Funders 0

None

BibTeX

@article{Van_Rossum_1999, title={Dynamic Interaction Between Soluble Tubulin and C‐Terminal Domains of N‐Methyl‐D‐Aspartate Receptor Subu}, volume={72}, ISSN={1471-4159}, url={http://dx.doi.org/10.1046/j.1471-4159.1999.0720962.x}, DOI={10.1046/j.1471-4159.1999.0720962.x}, number={3}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Van Rossum, Denise and Kuhse, Jochen and Betz, Heinrich}, year={1999}, month=mar, pages={962–973} }

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