DOI: 10.1046/j.1471-4159.1997.69062285.x. Cytotoxic Amyloid Peptides Inhibit Cellular 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐Diphenyltetrazolium Bromide (MTT) Reduction by Enhancing MTT Formazan Exocytosis

Cytotoxic Amyloid Peptides Inhibit Cellular 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐Diphenyltetrazolium Bromide (MTT) Reduction by Enhancing MTT Formazan Exocytosis

10.1046/j.1471-4159.1997.69062285.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: Amyloid β peptide (Aβ) neurotoxicity is believed to play a central role in the pathogenesis of Alzheimer's disease. An early indicator of Aβ toxicity is the inhibition of cellular 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) reduction to MTT formazan, a widely used assay for measuring cell viability. In this report we show that Aβ and other cytotoxic amyloid peptides such as human amylin dramatically enhance MTT formazan exocytosis, resulting in the inhibition of cellular MTT reduction. Only the amyloid peptides that are known to be cytotoxic enhanced MTT formazan exocytosis. Basal MTT formazan exocytosis and amyloid peptide‐enhanced MTT formazan exocytosis are blocked by several drugs with diverse known effects. These and other data suggest that MTT formazan exocytosis is a multistep process and that cytotoxic amyloid peptides enhance MTT formazan exocytosis through an intracellular signal transduction pathway.

Bibliography

Liu, Y., & Schubert, D. (1997). Cytotoxic Amyloid Peptides Inhibit Cellular 3â(4,5âDimethylthiazolâ2âyl)â2,5âDiphenyltetrazolium Bromide (MTT) Reduction by Enhancing MTT Formazan Exocytosis. Journal of Neurochemistry, 69(6), 2285â2293. Portico.

Authors 2

Yuanbin Liu (first)
David Schubert (additional)

References 0 Referenced 156

None

Dates

Type	When
Created	15 years, 1 month ago (July 15, 2010, 11:40 p.m.)
Deposited	1 year, 10 months ago (Oct. 27, 2023, 9:50 a.m.)
Indexed	1 month, 3 weeks ago (July 7, 2025, 2:03 a.m.)
Issued	27 years, 9 months ago (Dec. 1, 1997)
Published	27 years, 9 months ago (Dec. 1, 1997)
Published Online	22 years, 9 months ago (Nov. 18, 2002)
Published Print	27 years, 9 months ago (Dec. 1, 1997)

Funders 0

None

BibTeX

@article{Liu_1997, title={Cytotoxic Amyloid Peptides Inhibit Cellular 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐Diphenyltetrazolium Bromide (MTT) Reduction by Enhancing MTT Formazan Exocytosis}, volume={69}, ISSN={1471-4159}, url={http://dx.doi.org/10.1046/j.1471-4159.1997.69062285.x}, DOI={10.1046/j.1471-4159.1997.69062285.x}, number={6}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Liu, Yuanbin and Schubert, David}, year={1997}, month=dec, pages={2285–2293} }

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