DOI: 10.1046/j.1471-4159.1995.65051967.x. Modulation of a Recombinant Glycine Transporter (GLYT1b) by Activation of Protein Kinase C

Modulation of a Recombinant Glycine Transporter (GLYT1b) by Activation of Protein Kinase C

10.1046/j.1471-4159.1995.65051967.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: Treatment of human embryonic kidney cells (HEK 293 cells) expressing the mouse glycine transporter 1 (GLYT1b) with the protein kinase C (PKC) activator phorbol 12‐myristate 13‐acetate (PMA) decreased specific [3H]glycine uptake. This down‐regulation resulted from a reduction of the maximal transport rate and was blocked by the PKC inhibitors 1‐(5‐isoquinolinylsulfonyl)‐2‐methylpiperazine (H7) and staurosporine. The inhibitory effect of PMA treatment was also observed after removing all five predicted phosphorylation sites for PKC in GLYT1b by site‐directed mutagenesis. These data indicate that glycine transport by GLYT1b is modulated by PKC activation; however, this regulation may involve indirect phosphorylation mechanisms.

Bibliography

Sato, K., Adams, R., Betz, H., & Schloss, P. (1995). Modulation of a Recombinant Glycine Transporter (GLYT1b) by Activation of Protein Kinase C. Journal of Neurochemistry, 65(5), 1967â1973. Portico.

Authors 4

Kohji Sato (first)
Ralf Adams (additional)
Heinrich Betz (additional)
Patrick Schloss (additional)

References 0 Referenced 81

None

Dates

Type	When
Created	15 years, 1 month ago (July 15, 2010, 4:06 p.m.)
Deposited	1 year, 10 months ago (Oct. 26, 2023, 7:44 p.m.)
Indexed	1 month, 3 weeks ago (July 7, 2025, 1:45 a.m.)
Issued	29 years, 10 months ago (Nov. 1, 1995)
Published	29 years, 10 months ago (Nov. 1, 1995)
Published Online	22 years, 9 months ago (Nov. 23, 2002)
Published Print	29 years, 10 months ago (Nov. 1, 1995)

Funders 0

None

BibTeX

@article{Sato_1995, title={Modulation of a Recombinant Glycine Transporter (GLYT1b) by Activation of Protein Kinase C}, volume={65}, ISSN={1471-4159}, url={http://dx.doi.org/10.1046/j.1471-4159.1995.65051967.x}, DOI={10.1046/j.1471-4159.1995.65051967.x}, number={5}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Sato, Kohji and Adams, Ralf and Betz, Heinrich and Schloss, Patrick}, year={1995}, month=nov, pages={1967–1973} }

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