DOI: 10.1046/j.1471-4159.1994.62031074.x. Changes in the Extracellular Concentrations of Amino Acids in the Rat Striatum During Transient Focal Cerebral Ischemia

Changes in the Extracellular Concentrations of Amino Acids in the Rat Striatum During Transient Focal Cerebral Ischemia

10.1046/j.1471-4159.1994.62031074.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: Although considerable evidence supports a role for amino acids in transient global cerebral ischemia and permanent focal cerebral ischemia, effects of transient focal cerebral ischemia on the extracellular concentrations of amino acids have not been reported. Accordingly, our study was undertaken to examine the patterns of changes of extracellular glutamate, aspartate, GABA, taurine, glutamine, alanine, and phosphoethanolamine in the striatum of transient focal cerebral ischemia, as evidence to support their pathogenic roles. Focal ischemia was induced using the middle cerebral artery occlusion model, with no need for craniotomy. Microdialysis was used to sample the brain's extracellular space before, during, and after the ischemic period. One hour of middle cerebral artery occlusion followed by recirculation caused neuronal damage that was common in the frontoparietal cortex and the lateral segment of the caudate nucleus. During 1 h of ischemia, the largest increase occurred for GABA and moderate increases were observed for taurine, glutamate, and aspartate. Alanine, which is a nonneuroactive amino acid, increased little. After recirculation, the levels of glutamate and aspartate reverted to normal baseline values right after reperfusion. Despite these rapid normalizations, neuronal damage occurred. Therefore, uptake of excitatory amino acids can still be restored after 1 h of middle cerebral artery occlusion, and tissue damage occurs even though high extracellular levels of glutamate are not maintained.

Bibliography

UchiyamaâTsuyuki, Y., Araki, H., Yae, T., & Otomo, S. (1994). Changes in the Extracellular Concentrations of Amino Acids in the Rat Striatum During Transient Focal Cerebral Ischemia. Journal of Neurochemistry, 62(3), 1074â1078. Portico.

Authors 4

Yoshimi Uchiyama‐Tsuyuki (first)
Hiroaki Araki (additional)
Tetsuji Yae (additional)
Susumu Otomo (additional)

References 35 Referenced 63

10.1016/0165-3806(91)90214-4
10.1111/j.1471-4159.1991.tb08303.x
10.1111/j.1471-4159.1984.tb05396.x
10.1038/jcbfm.1989.90
10.1161/01.STR.20.7.904
10.1161/01.STR.21.12.1727
10.1523/JNEUROSCI.08-01-00185.1988
10.1097/00000542-198811000-00011
10.1111/j.1476-5381.1991.tb12443.x
10.1212/WNL.32.3.241
10.1016/0306-4522(88)90040-1
10.1111/j.1471-4159.1986.tb02836.x
10.1111/j.1471-4159.1988.tb01111.x
10.1111/j.1471-4159.1991.tb03775.x
10.1016/0304-3940(90)90799-F
10.1038/jcbfm.1989.87
10.1001/archneur.1988.00520260034016
10.3995/jstroke.8.1
10.1016/0304-3940(84)90185-X
10.1016/0006-8993(89)91514-X
10.1016/0306-4522(91)90035-M
10.1016/0361-9230(89)90202-5
10.1161/01.STR.20.8.1037
10.1016/0304-3940(86)90136-9
10.1038/jcbfm.1988.18
10.1038/jcbfm.1988.124
{'key': 'e_1_2_2_28_1', 'volume-title': 'The Rat Brain Stereotaxic Coordinates', 'author': 'Paxinos G.', 'year': '1986'} / The Rat Brain Stereotaxic Coordinates by Paxinos G. (1986)
10.1523/JNEUROSCI.09-02-00648.1989 / J. Neurosci. / Two distinct mechanisms, differentially affected by excitatory amino acids, trigger GABA release from fetal mouse striatal neurons in primary culture by Pin J. P. (1989)
10.1111/j.1471-4159.1979.tb04516.x
10.1016/0306-4522(80)90130-X
10.1016/0166-2236(87)90177-9
10.1111/j.1471-4159.1986.tb02847.x
10.1161/01.STR.20.2.281
10.1038/jcbfm.1981.6
10.1111/j.1471-4159.1983.tb08058.x

Dates

Type	When
Created	15 years, 1 month ago (July 15, 2010, 6:29 p.m.)
Deposited	1 year, 10 months ago (Oct. 24, 2023, 3:09 a.m.)
Indexed	1 month, 4 weeks ago (July 7, 2025, 1:45 a.m.)
Issued	31 years, 6 months ago (March 1, 1994)
Published	31 years, 6 months ago (March 1, 1994)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	31 years, 6 months ago (March 1, 1994)

Funders 0

None

BibTeX

@article{Uchiyama_Tsuyuki_1994, title={Changes in the Extracellular Concentrations of Amino Acids in the Rat Striatum During Transient Focal Cerebral Ischemia}, volume={62}, ISSN={1471-4159}, url={http://dx.doi.org/10.1046/j.1471-4159.1994.62031074.x}, DOI={10.1046/j.1471-4159.1994.62031074.x}, number={3}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Uchiyama‐Tsuyuki, Yoshimi and Araki, Hiroaki and Yae, Tetsuji and Otomo, Susumu}, year={1994}, month=mar, pages={1074–1078} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        7
      ]
    ],
    "date-time": "2025-07-07T05:45:58Z",
    "timestamp": 1751867158149
  },
  "reference-count": 35,
  "publisher": "Wiley",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2008,
            6,
            28
          ]
        ],
        "date-time": "2008-06-28T00:00:00Z",
        "timestamp": 1214611200000
      },
      "content-version": "vor",
      "delay-in-days": 5233,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1994,
        3
      ]
    ]
  },
  "abstract": "<jats:p><jats:bold>Abstract: </jats:bold> Although considerable evidence supports a role for amino acids in transient global cerebral ischemia and permanent focal cerebral ischemia, effects of transient focal cerebral ischemia on the extracellular concentrations of amino acids have not been reported. Accordingly, our study was undertaken to examine the patterns of changes of extracellular glutamate, aspartate, GABA, taurine, glutamine, alanine, and phosphoethanolamine in the striatum of transient focal cerebral ischemia, as evidence to support their pathogenic roles. Focal ischemia was induced using the middle cerebral artery occlusion model, with no need for craniotomy. Microdialysis was used to sample the brain's extracellular space before, during, and after the ischemic period. One hour of middle cerebral artery occlusion followed by recirculation caused neuronal damage that was common in the frontoparietal cortex and the lateral segment of the caudate nucleus. During 1 h of ischemia, the largest increase occurred for GABA and moderate increases were observed for taurine, glutamate, and aspartate. Alanine, which is a nonneuroactive amino acid, increased little. After recirculation, the levels of glutamate and aspartate reverted to normal baseline values right after reperfusion. Despite these rapid normalizations, neuronal damage occurred. Therefore, uptake of excitatory amino acids can still be restored after 1 h of middle cerebral artery occlusion, and tissue damage occurs even though high extracellular levels of glutamate are not maintained.</jats:p>",
  "DOI": "10.1046/j.1471-4159.1994.62031074.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2010,
        7,
        15
      ]
    ],
    "date-time": "2010-07-15T22:29:34Z",
    "timestamp": 1279232974000
  },
  "page": "1074-1078",
  "source": "Crossref",
  "is-referenced-by-count": 63,
  "title": "Changes in the Extracellular Concentrations of Amino Acids in the Rat Striatum During Transient Focal Cerebral Ischemia",
  "prefix": "10.1111",
  "volume": "62",
  "author": [
    {
      "given": "Yoshimi",
      "family": "Uchiyama\u2010Tsuyuki",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Hiroaki",
      "family": "Araki",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Tetsuji",
      "family": "Yae",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Susumu",
      "family": "Otomo",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2008,
        6,
        28
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_2_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0165-3806(91)90214-4"
    },
    {
      "key": "e_1_2_2_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1991.tb08303.x"
    },
    {
      "key": "e_1_2_2_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1984.tb05396.x"
    },
    {
      "key": "e_1_2_2_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/jcbfm.1989.90"
    },
    {
      "key": "e_1_2_2_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1161/01.STR.20.7.904"
    },
    {
      "key": "e_1_2_2_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1161/01.STR.21.12.1727"
    },
    {
      "key": "e_1_2_2_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.08-01-00185.1988"
    },
    {
      "key": "e_1_2_2_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1097/00000542-198811000-00011"
    },
    {
      "key": "e_1_2_2_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1476-5381.1991.tb12443.x"
    },
    {
      "key": "e_1_2_2_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1212/WNL.32.3.241"
    },
    {
      "key": "e_1_2_2_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0306-4522(88)90040-1"
    },
    {
      "key": "e_1_2_2_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1986.tb02836.x"
    },
    {
      "key": "e_1_2_2_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1988.tb01111.x"
    },
    {
      "key": "e_1_2_2_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1991.tb03775.x"
    },
    {
      "key": "e_1_2_2_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3940(90)90799-F"
    },
    {
      "key": "e_1_2_2_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/jcbfm.1989.87"
    },
    {
      "key": "e_1_2_2_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1001/archneur.1988.00520260034016"
    },
    {
      "key": "e_1_2_2_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.3995/jstroke.8.1"
    },
    {
      "key": "e_1_2_2_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3940(84)90185-X"
    },
    {
      "key": "e_1_2_2_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-8993(89)91514-X"
    },
    {
      "key": "e_1_2_2_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0306-4522(91)90035-M"
    },
    {
      "key": "e_1_2_2_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0361-9230(89)90202-5"
    },
    {
      "key": "e_1_2_2_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1161/01.STR.20.8.1037"
    },
    {
      "key": "e_1_2_2_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3940(86)90136-9"
    },
    {
      "key": "e_1_2_2_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/jcbfm.1988.18"
    },
    {
      "key": "e_1_2_2_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/jcbfm.1988.124"
    },
    {
      "key": "e_1_2_2_28_1",
      "volume-title": "The Rat Brain Stereotaxic Coordinates",
      "author": "Paxinos G.",
      "year": "1986"
    },
    {
      "key": "e_1_2_2_29_1",
      "doi-asserted-by": "crossref",
      "first-page": "648",
      "DOI": "10.1523/JNEUROSCI.09-02-00648.1989",
      "article-title": "Two distinct mechanisms, differentially affected by excitatory amino acids, trigger GABA release from fetal mouse striatal neurons in primary culture",
      "volume": "9",
      "author": "Pin J. P.",
      "year": "1989",
      "journal-title": "J. Neurosci."
    },
    {
      "key": "e_1_2_2_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1979.tb04516.x"
    },
    {
      "key": "e_1_2_2_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0306-4522(80)90130-X"
    },
    {
      "key": "e_1_2_2_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0166-2236(87)90177-9"
    },
    {
      "key": "e_1_2_2_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1986.tb02847.x"
    },
    {
      "key": "e_1_2_2_34_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1161/01.STR.20.2.281"
    },
    {
      "key": "e_1_2_2_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/jcbfm.1981.6"
    },
    {
      "key": "e_1_2_2_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1983.tb08058.x"
    }
  ],
  "container-title": "Journal of Neurochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1471-4159.1994.62031074.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1471-4159.1994.62031074.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        24
      ]
    ],
    "date-time": "2023-10-24T07:09:35Z",
    "timestamp": 1698131375000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1046/j.1471-4159.1994.62031074.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1994,
        3
      ]
    ]
  },
  "references-count": 35,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1994,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1046/j.1471-4159.1994.62031074.x"
  ],
  "URL": "http://dx.doi.org/10.1046/j.1471-4159.1994.62031074.x",
  "relation": {},
  "ISSN": [
    "0022-3042",
    "1471-4159"
  ],
  "subject": [],
  "container-title-short": "Journal of Neurochemistry",
  "published": {
    "date-parts": [
      [
        1994,
        3
      ]
    ]
  }
}