DOI: 10.1046/j.1471-4159.1994.62030873.x. Antipeptide Antibodies Against a <i>Torpedo</i> Cysteine‐String Protein

Antipeptide Antibodies Against a Torpedo Cysteine‐String Protein

10.1046/j.1471-4159.1994.62030873.x

Crossref journal-article

Wiley

Journal of Neurochemistry (311)

Abstract

Abstract: An antipeptide antiserum was raised against the C‐terminal undecapeptide of a Torpedo cysteine‐string protein (csp), a putative subunit or modulator of presynaptic calcium channels. This antiserum was shown to identify selectively the 27‐kDa in vitro translation product of the csp cRNA both by immunoprecipitation and on immunoblots. When affinity‐purified anti‐csp antibodies were used to probe immunoblots of membrane proteins from Torpedo electric organ or liver, specific immunoreactivity was detected only in electric organ. This immunoreactivity was associated principally with a single protein species of about 34 kDa. These results indicate that csp immunoreactivity is detectably expressed in electroplax, a heavily innervated tissue, but not in liver, which should have an appreciably lower abundance of presynaptic calcium channel proteins. Moreover, the increased relative molecular mass of csp in electric organ (compared with in vitro translated material) implies that csp is posttranslationally modified. Finally, immunoblot analysis of either intact, alkali‐treated, or solubilized membrane fractions of electric organ reveals that csp is predominantly a membrane protein.

Bibliography

Mastrogiacomo, A., Evans, C. J., & Gundersen, C. B. (1994). Antipeptide Antibodies Against a Torpedo CysteineâString Protein. Journal of Neurochemistry, 62(3), 873â880. Portico.

Authors 3

Alessandro Mastrogiacomo (first)
Christopher J. Evans (additional)
Cameron B. Gundersen (additional)

References 20 Referenced 23

10.1126/science.1321498
10.1016/S0021-9258(19)69848-0 / J. Biol Chem. / Phase separation of integral membrane proteins in Triton X‐1 14 solution by Bordier C. (1981)
10.1091/mbc.4.6.555
{'key': 'e_1_2_2_5_1', 'first-page': '503', 'volume-title': 'Selected Methods for Antibody and Nucleic Acid Probes', 'author': 'Evans C. J.', 'year': '1993'} / Selected Methods for Antibody and Nucleic Acid Probes by Evans C. J. (1993)
10.1073/pnas.86.16.6013
10.1016/0896-6273(92)90190-O
{'key': 'e_1_2_2_8_1', 'volume-title': 'Antibodies: A Laboratory Manual', 'author': 'Harlow E.', 'year': '1988'} / Antibodies: A Laboratory Manual by Harlow E. (1988)
10.1038/227680a0 / Nature / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 by Laemmli U. K. (1970)
10.1073/pnas.89.8.3625
10.1016/S0021-9258(19)39727-3 / J. Biol. Chem. / Physical interactions between bacteriophage and Escherichia coli proteins required for initiation of λ replication by Liberek K. (1990)
10.1083/jcb.91.1.257
10.1073/pnas.88.24.11095
10.1038/345260a0
10.1016/0006-8993(91)90942-O
10.1111/j.1749-6632.1991.tb36522.x
10.1016/0896-6273(92)90069-P
10.1073/pnas.88.18.7903
10.1126/science.1321501
10.1016/S0021-9258(19)73984-2
10.3109/01677069009084150

Dates

Type	When
Created	15 years, 1 month ago (July 15, 2010, 5:40 p.m.)
Deposited	1 year, 10 months ago (Oct. 24, 2023, 3:14 a.m.)
Indexed	1 year, 7 months ago (Jan. 15, 2024, 12:20 a.m.)
Issued	31 years, 5 months ago (March 1, 1994)
Published	31 years, 5 months ago (March 1, 1994)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	31 years, 5 months ago (March 1, 1994)

Funders 0

None

BibTeX

@article{Mastrogiacomo_1994, title={Antipeptide Antibodies Against a Torpedo Cysteine‐String Protein}, volume={62}, ISSN={1471-4159}, url={http://dx.doi.org/10.1046/j.1471-4159.1994.62030873.x}, DOI={10.1046/j.1471-4159.1994.62030873.x}, number={3}, journal={Journal of Neurochemistry}, publisher={Wiley}, author={Mastrogiacomo, Alessandro and Evans, Christopher J. and Gundersen, Cameron B.}, year={1994}, month=mar, pages={873–880} }

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