DOI: 10.1046/j.1460-9568.2000.00260.x. Expression of c‐Met in developing rat hippocampus: evidence for HGF as a neurotrophic factor for calbindin D‐expressing neurons

Expression of c‐Met in developing rat hippocampus: evidence for HGF as a neurotrophic factor for calbindin D‐expressing neurons

10.1046/j.1460-9568.2000.00260.x

Crossref journal-article

Wiley

European Journal of Neuroscience (311)

Abstract

AbstractHepatocyte growth factor‐scatter factor (HGF) is expressed in different parts of the nervous system, and has been shown to exhibit neurotrophic activity. Here we show that c‐Met, the receptor for HGF, is expressed in developing rat hippocampus, with the highest levels during the first postnatal weeks. To study the function of HGF, hippocampal neurons were prepared from embryonic rats and treated with different HGF concentrations. In these cultures, HGF increased the number of neurons expressing the 28‐kDa calcium‐binding protein (calbindin D) in a dose‐dependent manner. The effect of HGF was larger than that observed with either brain‐derived neurotrophic factor (BDNF) or neurotrophin‐3 (NT‐3), and cotreatment of the cultures with HGF and the neurotrophins was additive with respect to calbindin D neurons. Besides affecting the number of neurons, HGF significantly increased the degree of sprouting of calbindin D‐positive neurons, suggesting an influence on neuronal maturation. BDNF and NT‐3 stimulated neurite outgrowth of calbindin D neurons to a much smaller degree. In contrast to calbindin D neurons, HGF did not significantly increase the number of neurons immunoreactive with the neurotransmitter γ‐aminobutyric acid (GABA) in the hippocampal cultures. Immunohistochemical studies showed that c‐Met‐, calbindin D‐ and HGF‐immunoreactive cells are all present in the dentate gyrus and partly colocalize within neurons. These results show that HGF acts on calbindin D‐containing hippocampal neurons and increases their neurite outgrowth, suggesting that HGF plays an important role for the maturation and function of these neurons in the hippocampus.

Bibliography

Korhonen, L., SjÃ¶holm, U., Takei, N., Kern, M. A., Schirmacher, P., CastrÃ©n, E., & Lindholm, D. (2000). Expression of câMet in developing rat hippocampus: evidence for HGF as a neurotrophic factor for calbindin Dâexpressing neurons. European Journal of Neuroscience, 12(10), 3453â3461. Portico.

Authors 7

Laura Korhonen (first)
Ulrika Sjöholm (additional)
Nobuyuki Takei (additional)
Michael A. Kern (additional)
Peter Schirmacher (additional)
Eero Castrén (additional)
Dan Lindholm (additional)

References 50 Referenced 49

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Dates

Type	When
Created	22 years, 5 months ago (March 6, 2003, 9:27 a.m.)
Deposited	1 year, 10 months ago (Oct. 3, 2023, 4:14 p.m.)
Indexed	3 months, 1 week ago (May 20, 2025, 7:31 a.m.)
Issued	24 years, 10 months ago (Oct. 1, 2000)
Published	24 years, 10 months ago (Oct. 1, 2000)
Published Online	23 years, 8 months ago (Dec. 24, 2001)
Published Print	24 years, 10 months ago (Oct. 1, 2000)

Funders 0

None

BibTeX

@article{Korhonen_2000, title={Expression of c‐Met in developing rat hippocampus: evidence for HGF as a neurotrophic factor for calbindin D‐expressing neurons}, volume={12}, ISSN={1460-9568}, url={http://dx.doi.org/10.1046/j.1460-9568.2000.00260.x}, DOI={10.1046/j.1460-9568.2000.00260.x}, number={10}, journal={European Journal of Neuroscience}, publisher={Wiley}, author={Korhonen, Laura and Sjöholm, Ulrika and Takei, Nobuyuki and Kern, Michael A. and Schirmacher, Peter and Castrén, Eero and Lindholm, Dan}, year={2000}, month=oct, pages={3453–3461} }

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  "URL": "http://dx.doi.org/10.1046/j.1460-9568.2000.00260.x",
  "relation": {},
  "ISSN": [
    "0953-816X",
    "1460-9568"
  ],
  "subject": [],
  "container-title-short": "Eur J of Neuroscience",
  "published": {
    "date-parts": [
      [
        2000,
        10
      ]
    ]
  },
  "assertion": [
    {
      "value": "2001-12-24",
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        "label": "Publication History"
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    }
  ]
}