DOI: 10.1046/j.1439-0507.2000.00586.x. Multiple forms of the serine protease<i> Alp</i> of <i>Aspergillus fumigatus</i>

Multiple forms of the serine protease Alp of Aspergillus fumigatus

10.1046/j.1439-0507.2000.00586.x

Crossref journal-article

Wiley

Mycoses (311)

Abstract

SummaryAlkaline proteases were produced by a virulent strain of Aspergillus fumigatus during growth on media containing glucose and proteins or peptides. After isoelectric focusing, six bands of proteolytic activity were detected with synthetic substrates after blotting on nitrocellulose membranes. The main protease (pI=8.6) corresponded to the known subtilisin‐like protease Alp of A. fumigatus and five minor components had lower isoelectric points (8.1 to 5.2). All proteases were produced on different media and in various phases of growth with only small quantitative variations. They also had identical pH optima, were denatured above 45 °C and stabilized by Ca2+ ions, were affected by the inhibitors of serine proteases only and had nearly identical substrate specificity against 13 synthetic substrates. On gel chromatography the three most acidic components had higher molecular weights than the main enzyme Alp. It remains to be determined if the enzymes under study arise through post‐translational processing of the main protease or are true isoenzymes, products of a gene family.

Bibliography

Kunert, J., & KopeÄek, P. (2000). Multiple forms of the serine protease Alp of Aspergillus fumigatus. Mycoses, 43(9â10), 339â347. Portico.

Authors 2

J. Kunert (first)
P. Kopeček (additional)

References 42 Referenced 23

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10.1111/j.1574-6968.1992.tb05253.x
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10.1128/iai.61.10.4099-4104.1993 / Infect. Immun. / Isolation and characterization of a secreted metalloprotease of Aspergillus fumigatus. by Monod M. (1993)
10.1111/j.1365-2958.1994.tb01327.x
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{'key': 'e_1_2_6_15_2', 'first-page': '102', 'article-title': 'Chromatographic purification and homogeneity of extracellular acid proteinase of Aspergillus fumigatus.', 'volume': '29', 'author': 'Panneerselvam M.', 'year': '1980', 'journal-title': 'Ital. J. Biochem.'} / Ital. J. Biochem. / Chromatographic purification and homogeneity of extracellular acid proteinase of Aspergillus fumigatus. by Panneerselvam M. (1980)
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10.1080/02681219780001131
10.1128/jb.178.13.3899-3907.1996 / J. Bacteriol. / Disruption of the serine proteinase gene (sep) in Aspergillus flavus leads to a compensatory increase in the expression of a metalloprotease gene (mep 20). by Ramesh M. V. (1996)
10.1074/jbc.272.10.6238
10.1128/iai.65.8.3042-3047.1997 / Infect. Immun. / Dipeptidyl‐peptidase IV secreted by Aspergillus fumigatus, a fungus pathogenic to humans. by Beauvais A. (1997)
10.1016/0378-1119(95)00434-8
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{'key': 'e_1_2_6_34_2', 'first-page': '187', 'article-title': 'Some properties of the alkaline proteinase from Aspergillus melleus.', 'volume': '13', 'author': 'Luisetti M.', 'year': '1991', 'journal-title': 'Int. J. Tissue React.'} / Int. J. Tissue React. / Some properties of the alkaline proteinase from Aspergillus melleus. by Luisetti M. (1991)
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{'key': 'e_1_2_6_37_2', 'first-page': '125', 'article-title': 'Isolation and properties of the serine protease from Aspergillus oryzae (in Russian).', 'volume': '56', 'author': 'Vaganova T. I.', 'year': '1991', 'journal-title': 'Biokhimiya'} / Biokhimiya / Isolation and properties of the serine protease from Aspergillus oryzae (in Russian). by Vaganova T. I. (1991)
10.1016/0091-6749(94)90257-7
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10.1128/iai.62.3.936-942.1994 / Infect. Immun. / Recombinant expression and antigenic properties of a 32‐kilodalton extracellular alkaline protease, representing a possible virulence factor from Aspergillus fumigatus. by Moser M. (1994)
10.1099/00222615-39-5-393
10.1111/j.1574-6968.1995.tb07700.x

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 9:47 a.m.)
Deposited	1 year, 11 months ago (Sept. 1, 2023, 2:03 p.m.)
Indexed	1 year, 10 months ago (Oct. 10, 2023, 1:24 p.m.)
Issued	24 years, 10 months ago (Oct. 2, 2000)
Published	24 years, 10 months ago (Oct. 2, 2000)
Published Online	23 years, 8 months ago (Dec. 25, 2001)
Published Print	24 years, 10 months ago (Oct. 2, 2000)

Funders 0

None

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  "subject": [],
  "container-title-short": "Mycoses",
  "published": {
    "date-parts": [
      [
        2000,
        10,
        2
      ]
    ]
  }
}