DOI: 10.1046/j.1432-1327.2001.02263.x. Purified components of the <i>Escherichia coli</i> Tat protein transport system form a double‐layered ring structure

Purified components of the Escherichia coli Tat protein transport system form a double‐layered ring structure

10.1046/j.1432-1327.2001.02263.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The Escherichia coli twin arginine translocation (Tat) system mediates Sec‐independent export of protein precursors bearing twin arginine signal peptides. The genes tatA, tatB, tatC and tatE code for integral membrane proteins that are components of the Tat pathway. Cells co‐overexpressing tatABCDE show an increased rate of export of a signal peptide‐defective Tat precursor protein and a complex containing the TatA and TatB proteins can be purified from the membranes of such cells. The purified TatAB complex has an apparent molecular mass of 600 kDa as measured by gel permeation chromatography and, like the membranes of wild‐type cells, contains a large molar excess of TatA over TatB. Negative stain electron microscopy of the complex reveals cylindrical structures that may correspond to the Tat protein transport channel.

Bibliography

Sargent, F., Gohlke, U., de Leeuw, E., Stanley, N. R., Palmer, T., Saibil, H. R., & Berks, B. C. (2001). Purified components of the Escherichia coli Tat protein transport system form a doubleâlayered ring structure. European Journal of Biochemistry, 268(12), 3361â3367. Portico.

Authors 7

Frank Sargent (first)
Ulrich Gohlke (additional)
Erik de Leeuw (additional)
Nicola R. Stanley (additional)
Tracy Palmer (additional)
Helen R. Saibil (additional)
Ben C. Berks (additional)

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Dates

Type	When
Created	22 years, 5 months ago (March 11, 2003, 1:11 p.m.)
Deposited	1 year, 10 months ago (Oct. 16, 2023, 8:50 p.m.)
Indexed	1 year ago (Aug. 13, 2024, 4:31 a.m.)
Issued	24 years, 2 months ago (June 1, 2001)
Published	24 years, 2 months ago (June 1, 2001)
Published Online	23 years, 8 months ago (Dec. 20, 2001)
Published Print	24 years, 2 months ago (June 1, 2001)

Funders 0

None

BibTeX

@article{Sargent_2001, title={Purified components of the Escherichia coli Tat protein transport system form a double‐layered ring structure}, volume={268}, ISSN={1432-1033}, url={http://dx.doi.org/10.1046/j.1432-1327.2001.02263.x}, DOI={10.1046/j.1432-1327.2001.02263.x}, number={12}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Sargent, Frank and Gohlke, Ulrich and de Leeuw, Erik and Stanley, Nicola R. and Palmer, Tracy and Saibil, Helen R. and Berks, Ben C.}, year={2001}, month=jun, pages={3361–3367} }

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