DOI: 10.1046/j.1432-1327.2001.02111.x. Varying competence for protein import into chloroplasts during the cell cycle in <i>Chlamydomonas</i>

Varying competence for protein import into chloroplasts during the cell cycle in Chlamydomonas

10.1046/j.1432-1327.2001.02111.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

By studying the import of radioactively labelled small subunit of ribulose‐1,5‐bisphosphate carboxylase (pSS) into chloroplasts of the green alga C. reinhardtii cw‐15 protein delivery to chloroplasts was found to vary during the cell cycle. Chloroplasts were isolated from highly synchronous cultures at different time points during the cell cycle. When pSS was imported into ‘young’ chloroplasts isolated early in the light period about three times less pSS was processed to small subunit SS than in ‘mature’ chloroplasts from the middle of the light period. In ‘young’ chloroplasts also, less pSS was bound to the envelope surface. During the second half of the light period the import competence of isolated chloroplasts decreased again when based on chlorophyll content or cell volume, but did not change significantly when related to chloroplast number. Measurements of pSS binding to the surface of chloroplasts of different age indicated that the adaptation of protein import competence during the cell cycle is due to a variation of the number of binding sites per chloroplast surface area, rather than to modulation of the binding constant.

Bibliography

Su, Q., Schild, C., Schumann, P., & Boschetti, A. (2001). Varying competence for protein import into chloroplasts during the cell cycle in Chlamydomonas. European Journal of Biochemistry, 268(8), 2315â2321. Portico.

Authors 4

Qingxiang Su (first)
Christof Schild (additional)
Philipp Schumann (additional)
Arminio Boschetti (additional)

References 41 Referenced 8

10.1146/annurev.cellbio.12.1.1
10.1023/A:1006034020192
{'key': 'e_1_2_7_4_2', 'first-page': '222', 'article-title': 'Protein import into chloroplasts', 'volume': '9', 'author': 'Chen X.J.', 'year': '1999', 'journal-title': 'Trends Plant Sci.'} / Trends Plant Sci. / Protein import into chloroplasts by Chen X.J. (1999)
10.1016/S1369-5266(99)00021-7
10.1016/s0014-5793(99)00527-x
10.1073/pnas.84.10.3288
10.1016/S0021-9258(18)83489-5 / J. Biol. Chem. / ATP is required for the binding of precursor proteins of chloroplasts by Olsen L.J. (1989)
10.1016/S0021-9258(18)48513-4
10.1016/0014-5793(92)80186-K
10.1073/pnas.93.15.7684
10.1073/pnas.92.16.7177
10.1046/j.1365-313X.1998.00224.x
10.1073/pnas.95.13.7463
10.1042/bj3000787
10.1023/A:1006472325830
10.1016/S0021-9258(19)88620-9 / J. Biol. Chem. / Chloroplast envelope proteins are encoded by the chloroplast genome of Chlamydomonas reinahrdtii by Clemetson J.M. (1992)
10.1007/BF00039388
{'key': 'e_1_2_7_19_2', 'first-page': '113', 'article-title': 'The co‐ordination of nuclear and organellar genome expression in eukaryotic cells', 'volume': '32', 'author': 'Surpin M.', 'year': '1997', 'journal-title': 'Essays Biochem.'} / Essays Biochem. / The co‐ordination of nuclear and organellar genome expression in eukaryotic cells by Surpin M. (1997)
10.1016/s1369-5266(99)00013-8
10.1002/j.1460-2075.1987.tb02542.x
10.1073/pnas.94.25.14168
10.1074/jbc.271.3.1486
10.1128/MCB.17.6.3440
10.1126/science.7992056
10.1104/pp.121.4.1367
{'key': 'e_1_2_7_27_2', 'first-page': '743', 'article-title': 'Polyamines and cytoplasmic preparations enhance light‐driven protein synthesis in isolated chloroplasts of Chlamydomonas reinhardtii', 'volume': '30', 'author': 'Blättler R.', 'year': '1992', 'journal-title': 'Plant Physiol. Biochem.'} / Plant Physiol. Biochem. / Polyamines and cytoplasmic preparations enhance light‐driven protein synthesis in isolated chloroplasts of Chlamydomonas reinhardtii by Blättler R. (1992)
10.1073/pnas.46.1.83
10.1111/j.0022-3646.1997.00673.x
10.1016/0168-9452(85)90076-7
10.1016/s0014-5793(97)00016-1
10.1093/oxfordjournals.pcp.a029353
10.1042/0264-6021:3440391
10.1111/j.1432-1033.1988.tb14366.x-i2
10.1111/j.1432-1033.1993.tb18335.x
10.1104/pp.89.3.993
10.1038/350313a0
10.1111/j.1432-1033.1988.tb14241.x
10.1038/35003214
10.1105/tpc.3.10.1131
10.1073/pnas.94.16.8890
10.1073/pnas.160242597

Dates

Type	When
Created	22 years, 5 months ago (March 11, 2003, 1:28 p.m.)
Deposited	1 year, 10 months ago (Oct. 16, 2023, 8:39 p.m.)
Indexed	1 year, 10 months ago (Oct. 16, 2023, 9:12 p.m.)
Issued	24 years, 4 months ago (April 15, 2001)
Published	24 years, 4 months ago (April 15, 2001)
Published Online	23 years, 8 months ago (Dec. 20, 2001)
Published Print	24 years, 4 months ago (April 15, 2001)

Funders 0

None

BibTeX

@article{Su_2001, title={Varying competence for protein import into chloroplasts during the cell cycle in Chlamydomonas}, volume={268}, ISSN={1432-1033}, url={http://dx.doi.org/10.1046/j.1432-1327.2001.02111.x}, DOI={10.1046/j.1432-1327.2001.02111.x}, number={8}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Su, Qingxiang and Schild, Christof and Schumann, Philipp and Boschetti, Arminio}, year={2001}, month=apr, pages={2315–2321} }

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