DOI: 10.1046/j.1432-1327.2001.02082.x. Defined sequence segments of the small heat shock proteins HSP25 and αB‐crystallin inhibit actin polymerization

Defined sequence segments of the small heat shock proteins HSP25 and αB‐crystallin inhibit actin polymerization

10.1046/j.1432-1327.2001.02082.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The interaction of small heat shock proteins (sHSPs) with the actin cytoskeleton has been described and some members of this family, e.g. chicken and murine HSP25 (HSP27), inhibit the polymerization of actin in vitro. To analyse the molecular basis of this interaction, we synthesized a set of overlapping peptides covering the complete sequence of murine HSP25 and tested the effect of these peptides on actin polymerization in vitro by fluorescence spectroscopy and electron microscopy. Two peptides comprising the sequences W43 to R57 (peptide 6) and I92 to N106 (peptide 11) of HSP25 were found to be potent inhibitors of actin polymerization. Phosphorylation of N‐terminally extended peptide 11 at serine residues known to be phosphorylated in vivo resulted in decline of their inhibitory activity. Interestingly, peptides derived from the homologous peptide 11 sequence of murine αB‐crystallin showed the same behaviour. The results suggest that both HSP25 and αB‐crystallin have the potential to inhibit actin polymerization and that this activity is regulated by phosphorylation.

Bibliography

Wieske, M., Benndorf, R., Behlke, J., DÃ¶lling, R., Grelle, G., Bielka, H., & Lutsch, G. (2001). Defined sequence segments of the small heat shock proteins HSP25 and Î±Bâcrystallin inhibit actin polymerization. European Journal of Biochemistry, 268(7), 2083â2090. Portico.

Authors 7

Martin Wieske (first)
Rainer Benndorf (additional)
Joachim Behlke (additional)
Rudolf Dölling (additional)
Gerlinde Grelle (additional)
Heinz Bielka (additional)
Gudrun Lutsch (additional)

References 57 Referenced 61

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Dates

Type	When
Created	22 years, 5 months ago (March 11, 2003, 1:27 p.m.)
Deposited	1 year, 9 months ago (Oct. 31, 2023, 10:41 a.m.)
Indexed	2 months, 1 week ago (June 10, 2025, 2:44 p.m.)
Issued	24 years, 4 months ago (April 1, 2001)
Published	24 years, 4 months ago (April 1, 2001)
Published Online	23 years, 8 months ago (Dec. 20, 2001)
Published Print	24 years, 4 months ago (April 1, 2001)

Funders 0

None

BibTeX

@article{Wieske_2001, title={Defined sequence segments of the small heat shock proteins HSP25 and αB‐crystallin inhibit actin polymerization}, volume={268}, ISSN={1432-1033}, url={http://dx.doi.org/10.1046/j.1432-1327.2001.02082.x}, DOI={10.1046/j.1432-1327.2001.02082.x}, number={7}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Wieske, Martin and Benndorf, Rainer and Behlke, Joachim and Dölling, Rudolf and Grelle, Gerlinde and Bielka, Heinz and Lutsch, Gudrun}, year={2001}, month=apr, pages={2083–2090} }

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  "journal-issue": {
    "issue": "7",
    "published-print": {
      "date-parts": [
        [
          2001,
          4
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1046/j.1432-1327.2001.02082.x"
  ],
  "URL": "http://dx.doi.org/10.1046/j.1432-1327.2001.02082.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        2001,
        4
      ]
    ]
  }
}