DOI: 10.1046/j.1432-1327.2000.01566.x. Ca<sup>2+</sup> signalling and muscle disease

10.1046/j.1432-1327.2000.01566.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Transient elevations of intracellular Ca2+ play a signalling role in such complex cellular functions as contraction, secretion, fertilization, proliferation, metabolism, heartbeat and memory. However, prolonged elevation of Ca2+ above about 10 µm is deleterious to a cell and can activate apoptosis. In muscle, there is a narrow window of Ca2+ dysregulation in which abnormalities in Ca2+ regulatory proteins can lead to disease, rather than apoptosis. Key proteins in the regulation of muscle Ca2+ are the voltage‐dependent, dihydropyridine‐sensitive, l‐type Ca2+ channels located in the transverse tubule and Ca2+ release channels in the junctional terminal cisternae of the sarcoplasmic reticulum. Abnormalities in these proteins play a key role in malignant hyperthermia (MH), a toxic response to anesthetics, and in central core disease (CCD), a muscle myopathy. Sarco(endo)plasmic reticulum Ca2+ ATPases (SERCAs) return sarcoplasmic Ca2+ to the lumen of the sarcoplasmic reticulum. Loss of SERCA1a Ca2+ pump function is one cause of exercise‐induced impairment of the relaxation of skeletal muscle, in Brody disease. Phospholamban expressed in cardiac muscle and sarcolipin expressed in skeletal muscle regulate SERCA activity. Studies with knockout and transgenic mice show that gain of inhibitory function of phospholamban alters cardiac contractility and could be a causal feature in some cardiomyopathies. Calsequestrin, calreticulin, and a series of other acidic, lumenal, Ca2+ binding proteins provide a buffer for Ca2+ stored in the sarcoplasmic reticulum. Overexpression of cardiac calsequestrin leads to cardiomyopathy and ablation of calreticulin alters cardiac development.

Bibliography

MacLennan, D. H. (2000). Ca2+ signalling and muscle disease. European Journal of Biochemistry, 267(17), 5291â5297. Portico.

Authors 1

David H. MacLennan (first)

References 78 Referenced 200

10.1038/27094
{'key': 'e_1_2_14_3_2', 'first-page': '3', 'volume-title': 'Calcium as a Cellular Regulator', 'author': 'Williams R.J.P.', 'year': '1999'} / Calcium as a Cellular Regulator by Williams R.J.P. (1999)
10.1038/nsb0698-409
10.1021/bi963076
10.1016/0968-0004(96)80879-6
10.1126/science.281.5381.1309
10.1152/physrev.1997.77.3.699
10.1146/annurev.bi.56.070187.002143
10.1152/physrev.1998.78.4.921
10.1074/jbc.273.20.12360
10.1152/physrev.1991.71.1.129
10.1038/nsb0698-476
10.1126/science.1589759
10.1038/ng0993-46
10.1038/ng0993-51
10.1086/515454
10.1038/ng1096-191
10.1074/jbc.275.20.14985
10.1074/jbc.275.14.10538
10.1172/JCI1362
10.1083/jcb.144.5.857
10.1046/j.1432-1327.2000.01568.x
{'key': 'e_1_2_14_24_2', 'first-page': '179', 'volume-title': 'Thermoregulation: Pathology, Pharmacology and Therapy', 'author': 'Britt B.A.', 'year': '1991'} / Thermoregulation: Pathology, Pharmacology and Therapy by Britt B.A. (1991)
10.1007/BF00396370
10.1136/jnnp.38.12.1177
10.1002/mus.880120203
10.1093/brain/79.4.610
{'key': 'e_1_2_14_29_2', 'first-page': '597', 'article-title': 'Central core disease. Clinical features in 13 patients', 'volume': '97', 'author': 'Shuaib A.', 'year': '1987', 'journal-title': 'J. Comp. Pathol.'} / J. Comp. Pathol. / Central core disease. Clinical features in 13 patients by Shuaib A. (1987)
10.1016/S0140-6736(60)92665-9
10.1093/brain/93.1.133
10.1136/bmj.1.5848.272
10.1093/bja/60.3.303
10.1073/pnas.96.7.4164
10.1016/S0021-9258(18)77371-7
10.1006/geno.1996.0238
10.1038/343559a0
10.1038/343562a0
10.1007/BF00197703
10.1016/0888-7543(91)90461-M
{'key': 'e_1_2_14_41_2', 'first-page': '398', 'article-title': 'Refined genetic localization for central core disease', 'volume': '52', 'author': 'Mulley J.C.', 'year': '1993', 'journal-title': 'Am. J. Hum. Genet.'} / Am. J. Hum. Genet. / Refined genetic localization for central core disease by Mulley J.C. (1993)
10.1006/geno.1993.1302
10.1016/S0002-9343(98)00108-9
10.1097/00000542-199404000-00008
10.1007/BF03013380
10.1093/bja/56.11.1267
10.1213/00000539-198910000-00015
10.1111/j.1399-6576.1997.tb04820.x
10.1097/00000542-199803000-00006
10.1016/S0888-7543(05)80152-1
10.1097/00000542-199505000-00001
10.1097/00000542-199505000-00002
10.1093/hmg/6.6.953
10.1073/pnas.90.9.3993
10.1007/978-1-4613-1143-0_10
10.1074/jbc.272.42.26332
10.1074/jbc.274.2.693
10.1056/NEJM196907242810403
10.1006/geno.1995.1259
10.1016/0092-8674(86)90269-2
10.1002/ana.410200108
10.1212/WNL.38.5.812
10.1136/jnnp.51.11.1425
10.1172/JCI117393
10.1006/geno.1997.4967
{'key': 'e_1_2_14_66_2', 'first-page': 'H1344', 'article-title': 'Phospholamban mediates the beta‐adrenergic‐enhanced Ca2+ uptake in mammalian ventricular myocytes', 'volume': '261', 'author': 'Sham J.S.', 'year': '1991', 'journal-title': 'Am. J. Physiology'} / Am. J. Physiology / Phospholamban mediates the beta‐adrenergic‐enhanced Ca2+ uptake in mammalian ventricular myocytes by Sham J.S. (1991)
10.1161/01.RES.75.3.401
10.1161/01.RES.79.6.1059
10.1172/JCI118446
{'key': 'e_1_2_14_70_2', 'first-page': 'H785', 'article-title': 'β‐Adrenergic regulation of cAMP and protein phosphorylation in phospholamban‐knockout mouse hearts', 'volume': '272', 'author': 'Kiss E.', 'year': '1997', 'journal-title': 'Am. J. Physiol.'} / Am. J. Physiol. / β‐Adrenergic regulation of cAMP and protein phosphorylation in phospholamban‐knockout mouse hearts by Kiss E. (1997)
10.1016/S0021-9258(17)39667-9 / J. Biol. Chem. / Purification and characterization of phospholamban from canine cardiac sarcoplasmic reticulum by Jones L.R. (1985)
10.1002/j.1460-2075.1994.tb06801.x
10.1074/jbc.271.10.5941
10.1074/jbc.272.24.15061
10.1074/jbc.272.25.15872
10.1074/jbc.273.23.14238
10.1074/jbc.274.31.21503
10.1042/bj3440281 / Biochem. J. / Calreticulin: one protein, one gene, many functions. by Michalak M. (1999)
10.1016/S0092-8674(00)81573-1

Dates

Type	When
Created	22 years, 5 months ago (March 11, 2003, 12:52 p.m.)
Deposited	1 year, 10 months ago (Oct. 7, 2023, 3:09 a.m.)
Indexed	1 day, 4 hours ago (Aug. 29, 2025, 5:48 a.m.)
Issued	24 years, 11 months ago (Sept. 1, 2000)
Published	24 years, 11 months ago (Sept. 1, 2000)
Published Online	21 years, 10 months ago (Oct. 3, 2003)
Published Print	24 years, 11 months ago (Sept. 1, 2000)

Funders 0

None

BibTeX

@article{MacLennan_2000, title={Ca2+ signalling and muscle disease}, volume={267}, ISSN={1432-1033}, url={http://dx.doi.org/10.1046/j.1432-1327.2000.01566.x}, DOI={10.1046/j.1432-1327.2000.01566.x}, number={17}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={MacLennan, David H.}, year={2000}, month=sep, pages={5291–5297} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        29
      ]
    ],
    "date-time": "2025-08-29T09:48:55Z",
    "timestamp": 1756460935701
  },
  "reference-count": 78,
  "publisher": "Wiley",
  "issue": "17",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2003,
            10,
            3
          ]
        ],
        "date-time": "2003-10-03T00:00:00Z",
        "timestamp": 1065139200000
      },
      "content-version": "vor",
      "delay-in-days": 1127,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2000,
        9
      ]
    ]
  },
  "abstract": "<jats:p>Transient elevations of intracellular Ca<jats:sup>2+</jats:sup> play a signalling role in such complex cellular functions as contraction, secretion, fertilization, proliferation, metabolism, heartbeat and memory. However, prolonged elevation of Ca<jats:sup>2+</jats:sup> above about 10\u2003\u00b5<jats:sc>m</jats:sc> is deleterious to a cell and can activate apoptosis. In muscle, there is a narrow window of Ca<jats:sup>2+</jats:sup> dysregulation in which abnormalities in Ca<jats:sup>2+</jats:sup> regulatory proteins can lead to disease, rather than apoptosis. Key proteins in the regulation of muscle Ca<jats:sup>2+</jats:sup> are the voltage\u2010dependent, dihydropyridine\u2010sensitive, <jats:sc>l</jats:sc>\u2010type Ca<jats:sup>2+</jats:sup> channels located in the transverse tubule and Ca<jats:sup>2+</jats:sup> release channels in the junctional terminal cisternae of the sarcoplasmic reticulum. Abnormalities in these proteins play a key role in malignant hyperthermia (MH), a toxic response to anesthetics, and in central core disease (CCD), a muscle myopathy. Sarco(endo)plasmic reticulum Ca<jats:sup>2+</jats:sup> ATPases (SERCAs) return sarcoplasmic Ca<jats:sup>2+</jats:sup> to the lumen of the sarcoplasmic reticulum. Loss of SERCA1a Ca<jats:sup>2+</jats:sup> pump function is one cause of exercise\u2010induced impairment of the relaxation of skeletal muscle, in Brody disease. Phospholamban expressed in cardiac muscle and sarcolipin expressed in skeletal muscle regulate SERCA activity. Studies with knockout and transgenic mice show that gain of inhibitory function of phospholamban alters cardiac contractility and could be a causal feature in some cardiomyopathies. Calsequestrin, calreticulin, and a series of other acidic, lumenal, Ca<jats:sup>2+</jats:sup> binding proteins provide a buffer for Ca<jats:sup>2+</jats:sup> stored in the sarcoplasmic reticulum. Overexpression of cardiac calsequestrin leads to cardiomyopathy and ablation of calreticulin alters cardiac development.</jats:p>",
  "DOI": "10.1046/j.1432-1327.2000.01566.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        3,
        11
      ]
    ],
    "date-time": "2003-03-11T17:52:29Z",
    "timestamp": 1047405149000
  },
  "page": "5291-5297",
  "source": "Crossref",
  "is-referenced-by-count": 200,
  "title": "Ca<sup>2+</sup> signalling and muscle disease",
  "prefix": "10.1111",
  "volume": "267",
  "author": [
    {
      "given": "David H.",
      "family": "MacLennan",
      "sequence": "first",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2003,
        10,
        3
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_14_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/27094"
    },
    {
      "key": "e_1_2_14_3_2",
      "first-page": "3",
      "volume-title": "Calcium as a Cellular Regulator",
      "author": "Williams R.J.P.",
      "year": "1999"
    },
    {
      "key": "e_1_2_14_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nsb0698-409"
    },
    {
      "key": "e_1_2_14_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi963076"
    },
    {
      "key": "e_1_2_14_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0968-0004(96)80879-6"
    },
    {
      "key": "e_1_2_14_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.281.5381.1309"
    },
    {
      "key": "e_1_2_14_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/physrev.1997.77.3.699"
    },
    {
      "key": "e_1_2_14_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.56.070187.002143"
    },
    {
      "key": "e_1_2_14_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/physrev.1998.78.4.921"
    },
    {
      "key": "e_1_2_14_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.273.20.12360"
    },
    {
      "key": "e_1_2_14_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/physrev.1991.71.1.129"
    },
    {
      "key": "e_1_2_14_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nsb0698-476"
    },
    {
      "key": "e_1_2_14_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1589759"
    },
    {
      "key": "e_1_2_14_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/ng0993-46"
    },
    {
      "key": "e_1_2_14_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/ng0993-51"
    },
    {
      "key": "e_1_2_14_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1086/515454"
    },
    {
      "key": "e_1_2_14_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/ng1096-191"
    },
    {
      "key": "e_1_2_14_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.275.20.14985"
    },
    {
      "key": "e_1_2_14_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.275.14.10538"
    },
    {
      "key": "e_1_2_14_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1172/JCI1362"
    },
    {
      "key": "e_1_2_14_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.144.5.857"
    },
    {
      "key": "e_1_2_14_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1432-1327.2000.01568.x"
    },
    {
      "key": "e_1_2_14_24_2",
      "first-page": "179",
      "volume-title": "Thermoregulation: Pathology, Pharmacology and Therapy",
      "author": "Britt B.A.",
      "year": "1991"
    },
    {
      "key": "e_1_2_14_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00396370"
    },
    {
      "key": "e_1_2_14_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1136/jnnp.38.12.1177"
    },
    {
      "key": "e_1_2_14_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/mus.880120203"
    },
    {
      "key": "e_1_2_14_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/brain/79.4.610"
    },
    {
      "key": "e_1_2_14_29_2",
      "first-page": "597",
      "article-title": "Central core disease. Clinical features in 13 patients",
      "volume": "97",
      "author": "Shuaib A.",
      "year": "1987",
      "journal-title": "J. Comp. Pathol."
    },
    {
      "key": "e_1_2_14_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0140-6736(60)92665-9"
    },
    {
      "key": "e_1_2_14_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/brain/93.1.133"
    },
    {
      "key": "e_1_2_14_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1136/bmj.1.5848.272"
    },
    {
      "key": "e_1_2_14_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/bja/60.3.303"
    },
    {
      "key": "e_1_2_14_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.96.7.4164"
    },
    {
      "key": "e_1_2_14_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)77371-7"
    },
    {
      "key": "e_1_2_14_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/geno.1996.0238"
    },
    {
      "key": "e_1_2_14_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/343559a0"
    },
    {
      "key": "e_1_2_14_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/343562a0"
    },
    {
      "key": "e_1_2_14_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00197703"
    },
    {
      "key": "e_1_2_14_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0888-7543(91)90461-M"
    },
    {
      "key": "e_1_2_14_41_2",
      "first-page": "398",
      "article-title": "Refined genetic localization for central core disease",
      "volume": "52",
      "author": "Mulley J.C.",
      "year": "1993",
      "journal-title": "Am. J. Hum. Genet."
    },
    {
      "key": "e_1_2_14_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/geno.1993.1302"
    },
    {
      "key": "e_1_2_14_43_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0002-9343(98)00108-9"
    },
    {
      "key": "e_1_2_14_44_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1097/00000542-199404000-00008"
    },
    {
      "key": "e_1_2_14_45_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF03013380"
    },
    {
      "key": "e_1_2_14_46_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/bja/56.11.1267"
    },
    {
      "key": "e_1_2_14_47_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1213/00000539-198910000-00015"
    },
    {
      "key": "e_1_2_14_48_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-6576.1997.tb04820.x"
    },
    {
      "key": "e_1_2_14_49_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1097/00000542-199803000-00006"
    },
    {
      "key": "e_1_2_14_50_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0888-7543(05)80152-1"
    },
    {
      "key": "e_1_2_14_51_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1097/00000542-199505000-00001"
    },
    {
      "key": "e_1_2_14_52_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1097/00000542-199505000-00002"
    },
    {
      "key": "e_1_2_14_53_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/hmg/6.6.953"
    },
    {
      "key": "e_1_2_14_54_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.90.9.3993"
    },
    {
      "key": "e_1_2_14_55_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/978-1-4613-1143-0_10"
    },
    {
      "key": "e_1_2_14_56_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.42.26332"
    },
    {
      "key": "e_1_2_14_57_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.274.2.693"
    },
    {
      "key": "e_1_2_14_58_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1056/NEJM196907242810403"
    },
    {
      "key": "e_1_2_14_59_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/geno.1995.1259"
    },
    {
      "key": "e_1_2_14_60_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(86)90269-2"
    },
    {
      "key": "e_1_2_14_61_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/ana.410200108"
    },
    {
      "key": "e_1_2_14_62_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1212/WNL.38.5.812"
    },
    {
      "key": "e_1_2_14_63_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1136/jnnp.51.11.1425"
    },
    {
      "key": "e_1_2_14_64_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1172/JCI117393"
    },
    {
      "key": "e_1_2_14_65_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/geno.1997.4967"
    },
    {
      "key": "e_1_2_14_66_2",
      "first-page": "H1344",
      "article-title": "Phospholamban mediates the beta\u2010adrenergic\u2010enhanced Ca2+ uptake in mammalian ventricular myocytes",
      "volume": "261",
      "author": "Sham J.S.",
      "year": "1991",
      "journal-title": "Am. J. Physiology"
    },
    {
      "key": "e_1_2_14_67_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1161/01.RES.75.3.401"
    },
    {
      "key": "e_1_2_14_68_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1161/01.RES.79.6.1059"
    },
    {
      "key": "e_1_2_14_69_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1172/JCI118446"
    },
    {
      "key": "e_1_2_14_70_2",
      "first-page": "H785",
      "article-title": "\u03b2\u2010Adrenergic regulation of cAMP and protein phosphorylation in phospholamban\u2010knockout mouse hearts",
      "volume": "272",
      "author": "Kiss E.",
      "year": "1997",
      "journal-title": "Am. J. Physiol."
    },
    {
      "key": "e_1_2_14_71_2",
      "doi-asserted-by": "crossref",
      "first-page": "7721",
      "DOI": "10.1016/S0021-9258(17)39667-9",
      "article-title": "Purification and characterization of phospholamban from canine cardiac sarcoplasmic reticulum",
      "volume": "260",
      "author": "Jones L.R.",
      "year": "1985",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_14_72_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1994.tb06801.x"
    },
    {
      "key": "e_1_2_14_73_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.271.10.5941"
    },
    {
      "key": "e_1_2_14_74_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.24.15061"
    },
    {
      "key": "e_1_2_14_75_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.25.15872"
    },
    {
      "key": "e_1_2_14_76_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.273.23.14238"
    },
    {
      "key": "e_1_2_14_77_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.274.31.21503"
    },
    {
      "key": "e_1_2_14_78_2",
      "doi-asserted-by": "crossref",
      "first-page": "281",
      "DOI": "10.1042/bj3440281",
      "article-title": "Calreticulin: one protein, one gene, many functions.",
      "volume": "344",
      "author": "Michalak M.",
      "year": "1999",
      "journal-title": "Biochem. J."
    },
    {
      "key": "e_1_2_14_79_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81573-1"
    }
  ],
  "container-title": "European Journal of Biochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1432-1327.2000.01566.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1432-1327.2000.01566.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        7
      ]
    ],
    "date-time": "2023-10-07T07:09:09Z",
    "timestamp": 1696662549000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1046/j.1432-1327.2000.01566.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2000,
        9
      ]
    ]
  },
  "references-count": 78,
  "journal-issue": {
    "issue": "17",
    "published-print": {
      "date-parts": [
        [
          2000,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1046/j.1432-1327.2000.01566.x"
  ],
  "URL": "http://dx.doi.org/10.1046/j.1432-1327.2000.01566.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        2000,
        9
      ]
    ]
  }
}