DOI: 10.1046/j.1432-1327.2000.01255.x. Inhibition of inositol uptake in astrocytes by antisense oligonucleotides delivered by pH‐sensitive liposomes

Inhibition of inositol uptake in astrocytes by antisense oligonucleotides delivered by pH‐sensitive liposomes

10.1046/j.1432-1327.2000.01255.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

An oligonucleotide of 20 bases, complementary to a region of the sodium/myo‐inositol cotransporter (SMIT) mRNA, was used to investigate the uptake efficiency and activity of transferred antisense oligonucleotides with regard to substrate uptake. We compared the efficiency of oligonucleotide delivery after application of either free or liposome‐encapsulated material. Delivery of liposome‐encapsulated material (marker or oligonucleotides) into astrocytoma cells and primary astrocyte cultures was more effective with pH‐sensitive liposomes [dioleoylphosphatidylethanolamine (DOPE)/cholesteryl hemisuccinate (CHEMS)] than with non‐pH‐sensitive liposomes (soy lecithin) or free material in solution. Antisense activity was evaluated by determination of myo‐inositol uptake and detection of SMIT transcripts by RT‐PCR. Encapsulation of oligonucleotides in pH‐sensitive liposomes increased the inhibition of inositol uptake at least 50‐fold compared with application of free oligonucleotides in solution.

Bibliography

Lubrich, B., van Calker, D., & PeschkaâSÃ¼ss, R. (2000). Inhibition of inositol uptake in astrocytes by antisense oligonucleotides delivered by pHâsensitive liposomes. European Journal of Biochemistry, 267(8), 2432â2438. Portico.

Authors 3

Beate Lubrich (first)
Dietrich van Calker (additional)
Regine Peschka‐Süss (additional)

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Dates

Type	When
Created	22 years, 5 months ago (March 11, 2003, 1:06 p.m.)
Deposited	1 year, 10 months ago (Oct. 31, 2023, 11:46 a.m.)
Indexed	1 year, 3 months ago (May 17, 2024, 5:04 p.m.)
Issued	25 years, 5 months ago (April 1, 2000)
Published	25 years, 5 months ago (April 1, 2000)
Published Online	23 years, 8 months ago (Dec. 25, 2001)
Published Print	25 years, 5 months ago (April 1, 2000)

Funders 0

None

BibTeX

@article{Lubrich_2000, title={Inhibition of inositol uptake in astrocytes by antisense oligonucleotides delivered by pH‐sensitive liposomes}, volume={267}, ISSN={1432-1033}, url={http://dx.doi.org/10.1046/j.1432-1327.2000.01255.x}, DOI={10.1046/j.1432-1327.2000.01255.x}, number={8}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Lubrich, Beate and van Calker, Dietrich and Peschka‐Süss, Regine}, year={2000}, month=apr, pages={2432–2438} }

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