DOI: 10.1046/j.1432-1327.2000.00991.x. The multisubunit chloroplast RNA polymerase A from mustard (<i>Sinapis alba</i> L.)

The multisubunit chloroplast RNA polymerase A from mustard (Sinapis alba L.)

10.1046/j.1432-1327.2000.00991.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

We previously identified two multisubunit plastid RNA polymerases termed A and B. The B enzyme has a bacterial‐type polypeptide composition and is sensitive to the prokaryotic transcription inhibitor rifampicin (Rif); the A enzyme has a more complex subunit structure and is Rif‐resistant. Here we report results of N‐terminal sequencing and MS carried out with the A enzyme, which establish that the latter contains rpo gene products and is structurally related to the B enzyme. Furthermore, evidence is provided that the A enzyme can be converted into a Rif‐sensitive enzyme form in a phosphorylation‐dependent manner in vitro by a treatment that results in depletion of a β‐like subunit. Database searches using sequence information derived from additional polypeptides that are present in purified A preparations revealed sequence similarity with chloroplast proteins involved in RNA processing and redox control. This proteomics approach thus points to the complexity of the chloroplast transcription apparatus and its interconnections with post‐transcriptional and signalling mechanisms.

Bibliography

Pfannschmidt, T., Ogrzewalla, K., Baginsky, S., Sickmann, A., Meyer, H. E., & Link, G. (2000). The multisubunit chloroplast RNA polymeraseâA from mustard (Sinapisâalba L.). European Journal of Biochemistry, 267(1), 253â261. Portico.

Authors 6

Thomas Pfannschmidt (first)
Karsten Ogrzewalla (additional)
Sacha Baginsky (additional)
Albert Sickmann (additional)
Helmut E. Meyer (additional)
Gerhard Link (additional)

References 60 Referenced 89

10.1007/978-3-7091-9138-5_9
10.1007/BF00039389
10.1016/s1360-1385(97)01088-1
10.1104/pp.103.2.309
10.1016/s1360-1385(97)87981-2
10.1073/pnas.71.12.4839
10.1073/pnas.90.12.5509
10.1016/s0966-842x(97)01182-7
10.1016/s1360-1385(99)01407-7
10.1002/j.1460-2075.1991.tb04892.x
10.1002/j.1460-2075.1993.tb05688.x
10.1002/j.1460-2075.1996.tb00640.x
10.1126/science.277.5327.809
10.1105/tpc.11.5.911 / Plant Cell / Functional analysis of two maize cDNAs encoding T7‐like RNA polymerases. by Chang C.C. (1999)
10.1007/BF00024199
10.1080/07352689209382326
10.1007/BF00015612
10.1007/BF00039388
10.1016/0005-2787(67)90147-5
10.1016/0022-2836(88)90517-7
10.1016/S0021-9258(18)82407-3 / J. Biol. Chem. / Rifampicin region revisited. New rifampicin‐resistant and streptolydigin‐resistant mutants in the β subunit of Escherichia coli RNA polymerase. by Severinov K. (1993)
10.1007/s004380050621
10.1002/bies.950180608
10.1073/pnas.74.12.5463
10.1111/j.1432-1033.1985.tb08826.x
10.1093/nar/9.23.6505
10.1007/BF00029885
10.1038/227680a0
10.1016/S0076-6879(83)96021-4
10.1016/0076-6879(91)01019-X
10.1002/elps.1150190615
10.1023/A:1006177807844
10.1023/A:1005802909902
10.1016/0014-5793(90)80614-O
10.1146/annurev.cellbio.12.1.1
10.1105/tpc.8.8.1409
10.1073/pnas.87.4.1531
10.1093/nar/19.12.3431
10.1093/nar/25.12.2319
10.1016/B978-0-12-715007-9.50011-6
10.1080/07352689309382355
10.1073/pnas.92.11.5012
10.1038/38763
10.1038/385357a0
10.1016/0968-0004(92)90503-2
10.1146/annurev.arplant.49.1.249
10.1146/annurev.pp.43.060192.000503
10.1104/pp.117.2.565
10.1104/pp.109.4.1133
10.1073/pnas.93.20.11268
10.1016/S0092-8674(00)80147-6
10.1073/pnas.96.11.6161
10.1007/978-94-015-8927-7_10
10.1042/bj2860833
10.1046/j.1365-313x.1999.00465.x
10.1016/0014-5793(86)80516-6
10.1016/0022-2836(88)90477-9
10.1007/BF00259403
10.1007/BF02464880
10.1104/pp.96.4.1393

Dates

Type	When
Created	22 years, 5 months ago (March 11, 2003, 12:43 p.m.)
Deposited	1 year, 10 months ago (Oct. 16, 2023, 2:03 a.m.)
Indexed	2 months ago (July 2, 2025, 11:39 a.m.)
Issued	25 years, 8 months ago (Jan. 1, 2000)
Published	25 years, 8 months ago (Jan. 1, 2000)
Published Online	23 years, 8 months ago (Dec. 25, 2001)
Published Print	25 years, 8 months ago (Jan. 1, 2000)

Funders 0

None

BibTeX

@article{Pfannschmidt_2000, title={The multisubunit chloroplast RNA polymerase A from mustard (Sinapis alba L.): Integration of a prokaryotic core into a larger complex with organelle‐specific functions}, volume={267}, ISSN={1432-1033}, url={http://dx.doi.org/10.1046/j.1432-1327.2000.00991.x}, DOI={10.1046/j.1432-1327.2000.00991.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Pfannschmidt, Thomas and Ogrzewalla, Karsten and Baginsky, Sacha and Sickmann, Albert and Meyer, Helmut E. and Link, Gerhard}, year={2000}, month=jan, pages={253–261} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        2
      ]
    ],
    "date-time": "2025-07-02T15:39:14Z",
    "timestamp": 1751470754033
  },
  "reference-count": 60,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2001,
            12,
            25
          ]
        ],
        "date-time": "2001-12-25T00:00:00Z",
        "timestamp": 1009238400000
      },
      "content-version": "vor",
      "delay-in-days": 724,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2000,
        1
      ]
    ]
  },
  "abstract": "<jats:p>We previously identified two multisubunit plastid RNA polymerases termed A and B. The B enzyme has a bacterial\u2010type polypeptide composition and is sensitive to the prokaryotic transcription inhibitor rifampicin (Rif); the A enzyme has a more complex subunit structure and is Rif\u2010resistant. Here we report results of N\u2010terminal sequencing and MS carried out with the A enzyme, which establish that the latter contains <jats:italic>rpo</jats:italic> gene products and is structurally related to the B enzyme. Furthermore, evidence is provided that the A enzyme can be converted into a Rif\u2010sensitive enzyme form in a phosphorylation\u2010dependent manner <jats:italic>in vitro</jats:italic> by a treatment that results in depletion of a \u03b2\u2010like subunit. Database searches using sequence information derived from additional polypeptides that are present in purified A preparations revealed sequence similarity with chloroplast proteins involved in RNA processing and redox control. This proteomics approach thus points to the complexity of the chloroplast transcription apparatus and its interconnections with post\u2010transcriptional and signalling mechanisms.</jats:p>",
  "DOI": "10.1046/j.1432-1327.2000.00991.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        3,
        11
      ]
    ],
    "date-time": "2003-03-11T17:43:28Z",
    "timestamp": 1047404608000
  },
  "page": "253-261",
  "source": "Crossref",
  "is-referenced-by-count": 89,
  "title": "The multisubunit chloroplast RNA polymerase\u2003A from mustard (<i>Sinapis\u2003alba</i> L.)",
  "prefix": "10.1111",
  "volume": "267",
  "author": [
    {
      "given": "Thomas",
      "family": "Pfannschmidt",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Karsten",
      "family": "Ogrzewalla",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Sacha",
      "family": "Baginsky",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Albert",
      "family": "Sickmann",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Helmut E.",
      "family": "Meyer",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Gerhard",
      "family": "Link",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2001,
        12,
        25
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_7_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/978-3-7091-9138-5_9"
    },
    {
      "key": "e_1_2_7_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00039389"
    },
    {
      "key": "e_1_2_7_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/s1360-1385(97)01088-1"
    },
    {
      "key": "e_1_2_7_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.103.2.309"
    },
    {
      "key": "e_1_2_7_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/s1360-1385(97)87981-2"
    },
    {
      "key": "e_1_2_7_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.71.12.4839"
    },
    {
      "key": "e_1_2_7_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.90.12.5509"
    },
    {
      "key": "e_1_2_7_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/s0966-842x(97)01182-7"
    },
    {
      "key": "e_1_2_7_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/s1360-1385(99)01407-7"
    },
    {
      "key": "e_1_2_7_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1991.tb04892.x"
    },
    {
      "key": "e_1_2_7_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1993.tb05688.x"
    },
    {
      "key": "e_1_2_7_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1996.tb00640.x"
    },
    {
      "key": "e_1_2_7_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.277.5327.809"
    },
    {
      "key": "e_1_2_7_15_2",
      "doi-asserted-by": "crossref",
      "first-page": "911",
      "DOI": "10.1105/tpc.11.5.911",
      "article-title": "Functional analysis of two maize cDNAs encoding T7\u2010like RNA polymerases.",
      "volume": "11",
      "author": "Chang C.C.",
      "year": "1999",
      "journal-title": "Plant Cell"
    },
    {
      "key": "e_1_2_7_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00024199"
    },
    {
      "key": "e_1_2_7_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1080/07352689209382326"
    },
    {
      "key": "e_1_2_7_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00015612"
    },
    {
      "key": "e_1_2_7_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00039388"
    },
    {
      "key": "e_1_2_7_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2787(67)90147-5"
    },
    {
      "key": "e_1_2_7_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(88)90517-7"
    },
    {
      "key": "e_1_2_7_22_2",
      "doi-asserted-by": "crossref",
      "first-page": "14820",
      "DOI": "10.1016/S0021-9258(18)82407-3",
      "article-title": "Rifampicin region revisited. New rifampicin\u2010resistant and streptolydigin\u2010resistant mutants in the \u03b2 subunit of Escherichia coli RNA polymerase.",
      "volume": "268",
      "author": "Severinov K.",
      "year": "1993",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_7_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s004380050621"
    },
    {
      "key": "e_1_2_7_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/bies.950180608"
    },
    {
      "key": "e_1_2_7_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.74.12.5463"
    },
    {
      "key": "e_1_2_7_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1985.tb08826.x"
    },
    {
      "key": "e_1_2_7_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/9.23.6505"
    },
    {
      "key": "e_1_2_7_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00029885"
    },
    {
      "key": "e_1_2_7_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/227680a0"
    },
    {
      "key": "e_1_2_7_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0076-6879(83)96021-4"
    },
    {
      "key": "e_1_2_7_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0076-6879(91)01019-X"
    },
    {
      "key": "e_1_2_7_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/elps.1150190615"
    },
    {
      "key": "e_1_2_7_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1023/A:1006177807844"
    },
    {
      "key": "e_1_2_7_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1023/A:1005802909902"
    },
    {
      "key": "e_1_2_7_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(90)80614-O"
    },
    {
      "key": "e_1_2_7_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.cellbio.12.1.1"
    },
    {
      "key": "e_1_2_7_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1105/tpc.8.8.1409"
    },
    {
      "key": "e_1_2_7_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.87.4.1531"
    },
    {
      "key": "e_1_2_7_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/19.12.3431"
    },
    {
      "key": "e_1_2_7_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/25.12.2319"
    },
    {
      "key": "e_1_2_7_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/B978-0-12-715007-9.50011-6"
    },
    {
      "key": "e_1_2_7_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1080/07352689309382355"
    },
    {
      "key": "e_1_2_7_43_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.92.11.5012"
    },
    {
      "key": "e_1_2_7_44_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/38763"
    },
    {
      "key": "e_1_2_7_45_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/385357a0"
    },
    {
      "key": "e_1_2_7_46_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0968-0004(92)90503-2"
    },
    {
      "key": "e_1_2_7_47_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.arplant.49.1.249"
    },
    {
      "key": "e_1_2_7_48_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.pp.43.060192.000503"
    },
    {
      "key": "e_1_2_7_49_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.117.2.565"
    },
    {
      "key": "e_1_2_7_50_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.109.4.1133"
    },
    {
      "key": "e_1_2_7_51_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.93.20.11268"
    },
    {
      "key": "e_1_2_7_52_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80147-6"
    },
    {
      "key": "e_1_2_7_53_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.96.11.6161"
    },
    {
      "key": "e_1_2_7_54_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/978-94-015-8927-7_10"
    },
    {
      "key": "e_1_2_7_55_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj2860833"
    },
    {
      "key": "e_1_2_7_56_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-313x.1999.00465.x"
    },
    {
      "key": "e_1_2_7_57_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(86)80516-6"
    },
    {
      "key": "e_1_2_7_58_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(88)90477-9"
    },
    {
      "key": "e_1_2_7_59_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00259403"
    },
    {
      "key": "e_1_2_7_60_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF02464880"
    },
    {
      "key": "e_1_2_7_61_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.96.4.1393"
    }
  ],
  "container-title": "European Journal of Biochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1432-1327.2000.00991.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1432-1327.2000.00991.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        16
      ]
    ],
    "date-time": "2023-10-16T06:03:06Z",
    "timestamp": 1697436186000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1046/j.1432-1327.2000.00991.x"
    }
  },
  "subtitle": [
    "Integration of a prokaryotic core into a larger complex with organelle\u2010specific functions"
  ],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2000,
        1
      ]
    ]
  },
  "references-count": 60,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          2000,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1046/j.1432-1327.2000.00991.x"
  ],
  "URL": "http://dx.doi.org/10.1046/j.1432-1327.2000.00991.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        2000,
        1
      ]
    ]
  }
}