DOI: 10.1046/j.1432-1327.1999.00410.x. The noncatalytic site‐deficient α3β3γ subcomplex and FoF1‐ATP synthase can continuously catalyse ATP hydrolysis when Pi is present

The noncatalytic site‐deficient α3β3γ subcomplex and FoF1‐ATP synthase can continuously catalyse ATP hydrolysis when Pi is present

10.1046/j.1432-1327.1999.00410.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

We investigated ATP hydrolysis by a mutant (ΔNC) α3β3γ subcomplex of F0F1‐ATP synthase from the thermophilic Bacillus PS3 that is defective in the noncatalytic nucleotide binding sites. This mutant subcomplex was activated by inorganic phosphate ions (Pi) and did not show continuous ATP hydrolysis activity in the absence of Pi. Pi also activated the wild‐type α3β3γ subcomplex in a similar manner. Sulphate activated wild‐type α3β3γ but not ΔNC α3β3γ, indicating that Pi activation did not involve noncatalytic sites but that sulphate activation did. Pi also activated ATP hydrolysis and coupled proton translocation by the wild‐type and ΔNC F0F1‐ATP synthases reconstituted into vesicle membranes.

Bibliography

Bald, D., Muneyuki, E., Amano, T., Kruip, J., Hisabori, T., & Yoshida, M. (1999). The noncatalytic siteâdeficient Î±3Î²3Î³ subcomplex and FoF1âATP synthase can continuously catalyse ATP hydrolysis when Pi is present. European Journal of Biochemistry, 262(2), 563â568. Portico.

Authors 6

Dirk Bald (first)
Eiro Muneyuki (additional)
Toyoki Amano (additional)
Jochen Kruip (additional)
Toru Hisabori (additional)
Masasuke Yoshida (additional)

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Dates

Type	When
Created	22 years, 5 months ago (March 11, 2003, 12:23 p.m.)
Deposited	1 year, 10 months ago (Oct. 16, 2023, 10:45 a.m.)
Indexed	1 year, 9 months ago (Oct. 31, 2023, 1:30 p.m.)
Issued	26 years, 2 months ago (June 1, 1999)
Published	26 years, 2 months ago (June 1, 1999)
Published Online	23 years, 8 months ago (Dec. 25, 2001)
Published Print	26 years, 2 months ago (June 1, 1999)

Funders 0

None

BibTeX

@article{Bald_1999, title={The noncatalytic site‐deficient α3β3γ subcomplex and FoF1‐ATP synthase can continuously catalyse ATP hydrolysis when Pi is present}, volume={262}, ISSN={1432-1033}, url={http://dx.doi.org/10.1046/j.1432-1327.1999.00410.x}, DOI={10.1046/j.1432-1327.1999.00410.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Bald, Dirk and Muneyuki, Eiro and Amano, Toyoki and Kruip, Jochen and Hisabori, Toru and Yoshida, Masasuke}, year={1999}, month=jun, pages={563–568} }

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