DOI: 10.1046/j.1432-1327.1999.00029.x. A new exon in the 5′ untranslated region of the <i>connexin32</i> gene

A new exon in the 5′ untranslated region of the connexin32 gene

10.1046/j.1432-1327.1999.00029.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The cloning and sequencing of two bovine connexin32 cDNAs are reported. Comparative analysis with known corresponding mammalian cDNA and protein sequences, besides confirming a high degree of similarity among these proteins, allowed us to identify some specific features of the bovine connexin32 gene. The latter include: the presence of a novel exon in the 5′ UTR which is alternatively spliced, giving rise to a new mRNA species; the presence of two potential hairpin loops in the 5′ and 3′ UTR; and the presence of an additional amino acid, glycine235, in the C‐terminal domain of the 284 residue protein. Among the common features, the presence of polypyrimidine clusters within the 3′ UTR, containing a consensus sequence for a cis‐acting element, is noteworthy. Expression of connexin32 mRNAs was analysed in 16 bovine tissues. Transcript analysis suggests the presence, in cattle, of an alternative downstream promoter.

Bibliography

Duga, S., Asselta, R., Del Giacco, L., Malcovati, M., Ronchi, S., Tenchini, M. L., & Simonic, T. (1999). A new exon in the 5â² untranslated region of the connexin32 gene. European Journal of Biochemistry, 259(1â2), 188â196. Portico.

Authors 7

Stefano Duga (first)
Rosanna Asselta (additional)
Luca Del Giacco (additional)
Massimo Malcovati (additional)
Severino Ronchi (additional)
Maria L. Tenchini (additional)
Tatjana Simonic (additional)

References 47 Referenced 21

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Dates

Type	When
Created	22 years, 5 months ago (March 11, 2003, 12:12 p.m.)
Deposited	2 years ago (Aug. 31, 2023, 8:03 a.m.)
Indexed	1 year, 11 months ago (Sept. 28, 2023, 1:05 a.m.)
Issued	26 years, 8 months ago (Jan. 1, 1999)
Published	26 years, 8 months ago (Jan. 1, 1999)
Published Online	23 years, 8 months ago (Dec. 25, 2001)
Published Print	26 years, 8 months ago (Jan. 1, 1999)

Funders 0

None

BibTeX

@article{Duga_1999, title={A new exon in the 5′ untranslated region of the connexin32 gene}, volume={259}, ISSN={1432-1033}, url={http://dx.doi.org/10.1046/j.1432-1327.1999.00029.x}, DOI={10.1046/j.1432-1327.1999.00029.x}, number={1–2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Duga, Stefano and Asselta, Rosanna and Del Giacco, Luca and Malcovati, Massimo and Ronchi, Severino and Tenchini, Maria L. and Simonic, Tatjana}, year={1999}, month=jan, pages={188–196} }

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