DOI: 10.1046/j.1432-1033.2002.03166.x. Kinetics of violaxanthin de‐epoxidation by violaxanthin de‐epoxidase, a xanthophyll cycle enzyme, is regulated by membrane fluidity in model lipid bilayers

Kinetics of violaxanthin de‐epoxidation by violaxanthin de‐epoxidase, a xanthophyll cycle enzyme, is regulated by membrane fluidity in model lipid bilayers

10.1046/j.1432-1033.2002.03166.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

This paper describes violaxanthin de‐epoxidation in model lipid bilayers. Unilamellar egg yolk phosphatidylcholine (PtdCho) vesicles supplemented with monogalactosyldiacylglycerol were found to be a suitable system for studying this reaction. Such a system resembles more the native thylakoid membrane and offers better possibilities for studying kinetics and factors controlling de‐epoxidation of violaxanthin than a system composed only ofmonogalactosyldiacylglycerol and is commonly used in xanthophyll cycle studies. The activity of violaxanthin de‐epoxidase (VDE) strongly depended on the ratio of monogalactosyldiacylglycerol to PtdCho in liposomes. The mathematical model of violaxanthin de‐epoxidation was applied to calculate the probability of violaxanthin to zeaxanthin conversion at different phases of de‐epoxidation reactions. Measurements of deepoxidation rate and EPR‐spin label study at different temperatures revealed that dynamic properties of the membrane are important factors that might control conversion of violaxanthin to antheraxanthin. A model of the molecular mechanism of violaxanthin de‐epoxidation where the reversed hexagonal structures (mainly created by monogalactosyldiacylglycerol) are assumed to be required for violaxanthin conversion to zeaxanthin is proposed. The presence of monogalactosyldiacylglycerol reversed hexagonal phase was detected in the PtdCho/monogalactosyldiacylglycerol liposomes membrane by 31P‐NMR studies. The availability of violaxanthin for de‐epoxidation is a diffusion‐dependent process controlled by membrane fluidity. The significance of the presented results for understanding themechanism of violaxanthin de‐epoxidation in native thylakoid membranes is discussed.

Bibliography

Latowski, D., Kruk, J., Burda, K., SkrzyneckaâJaskier, M., KosteckaâGugaÅa, A., & StrzaÅka, K. (2002). Kinetics of violaxanthin deâepoxidation by violaxanthin deâepoxidase, a xanthophyll cycle enzyme, is regulated by membrane fluidity in model lipid bilayers. European Journal of Biochemistry, 269(18), 4656â4665. Portico.

Authors 6

Dariusz Latowski (first)
Jerzy Kruk (additional)
Kvetoslava Burda (additional)
Marta Skrzynecka‐Jaskier (additional)
Anna Kostecka‐Gugała (additional)
Kazimierz Strzałka (additional)

References 36 Referenced 80

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{'key': 'e_1_2_18_37_2', 'first-page': '103', 'article-title': 'Modulation of thylakoid membrane fluidity by exogenously added carotenoids', 'volume': '1', 'author': 'Strzalka K.', 'year': '1997', 'journal-title': 'J. Biochem. Mol. Biol. Biophys.'} / J. Biochem. Mol. Biol. Biophys. / Modulation of thylakoid membrane fluidity by exogenously added carotenoids by Strzalka K. (1997)

Dates

Type	When
Created	22 years, 5 months ago (March 11, 2003, 1:34 p.m.)
Deposited	1 year, 10 months ago (Oct. 16, 2023, 11:02 p.m.)
Indexed	2 weeks, 6 days ago (Aug. 7, 2025, 4:43 p.m.)
Issued	22 years, 11 months ago (Sept. 1, 2002)
Published	22 years, 11 months ago (Sept. 1, 2002)
Published Online	22 years, 11 months ago (Sept. 11, 2002)
Published Print	22 years, 11 months ago (Sept. 1, 2002)

Funders 0

None

BibTeX

@article{Latowski_2002, title={Kinetics of violaxanthin de‐epoxidation by violaxanthin de‐epoxidase, a xanthophyll cycle enzyme, is regulated by membrane fluidity in model lipid bilayers}, volume={269}, ISSN={1432-1033}, url={http://dx.doi.org/10.1046/j.1432-1033.2002.03166.x}, DOI={10.1046/j.1432-1033.2002.03166.x}, number={18}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Latowski, Dariusz and Kruk, Jerzy and Burda, Kvetoslava and Skrzynecka‐Jaskier, Marta and Kostecka‐Gugała, Anna and Strzałka, Kazimierz}, year={2002}, month=sep, pages={4656–4665} }

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