DOI: 10.1046/j.1365-313x.2001.00958.x. Plastid‐expressed 5‐enolpyruvylshikimate‐3‐phosphate synthase genes provide high level glyphosate tolerance in tobacco

Plastid‐expressed 5‐enolpyruvylshikimate‐3‐phosphate synthase genes provide high level glyphosate tolerance in tobacco

10.1046/j.1365-313x.2001.00958.x

Crossref journal-article

Wiley

The Plant Journal (311)

Abstract

SummaryPlastid transformation (transplastomic) technology has several potential advantages for biotechnological applications including the use of unmodified prokaryotic genes for engineering, potential high‐level gene expression and gene containment due to maternal inheritance in most crop plants. However, the efficacy of a plastid‐encoded trait may change depending on plastid number and tissue type. We report a feasibility study in tobacco plastids to achieve high‐level herbicide resistance in both vegetative tissues and reproductive organs. We chose to test glyphosate resistance via over‐expression in plastids of tolerant forms of 5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSPS). Immunological, enzymatic and whole‐plant assays were used to prove the efficacy of three different prokaryotic (Achromobacter, Agrobacterium and Bacillus) EPSPS genes. Using the Agrobacterium strain CP4 EPSPS as a model we identified translational control sequences that direct a 10 000‐fold range of protein accumulation (to >10% total soluble protein in leaves). Plastid‐expressed EPSPS could provide very high levels of glyphosate resistance, although levels of resistance in vegetative and reproductive tissues differed depending on EPSPS accumulation levels, and correlated to the plastid abundance in these tissues. Paradoxically, higher levels of plastid‐expressed EPSPS protein accumulation were apparently required for efficacy than from a similar nuclear‐encoded gene. Nevertheless, the demonstration of high‐level glyphosate tolerance in vegetative and reproductive organs using transplastomic technology provides a necessary step for transfer of this technology to other crop species.

Bibliography

Ye, G., Hajdukiewicz, P. T. J., Broyles, D., Rodriguez, D., Xu, C. W., Nehra, N., & Staub, J. M. (2001). Plastidâexpressed 5âenolpyruvylshikimateâ3âphosphate synthase genes provide high level glyphosate tolerance in tobacco. The Plant Journal, 25(3), 261â270. Portico.

Authors 7

Guang‐Ning Ye (first)
Peter T.J. Hajdukiewicz (additional)
Debra Broyles (additional)
Damian Rodriguez (additional)
Charles W. Xu (additional)
Narender Nehra (additional)
Jeffrey M. Staub (additional)

References 35 Referenced 173

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 5:03 p.m.)
Deposited	1 year, 10 months ago (Oct. 17, 2023, 7:43 a.m.)
Indexed	1 month, 2 weeks ago (July 16, 2025, 8:49 a.m.)
Issued	24 years, 7 months ago (Feb. 1, 2001)
Published	24 years, 7 months ago (Feb. 1, 2001)
Published Online	23 years, 8 months ago (Dec. 23, 2001)
Published Print	24 years, 7 months ago (Feb. 1, 2001)

Funders 0

None

BibTeX

@article{Ye_2001, title={Plastid‐expressed 5‐enolpyruvylshikimate‐3‐phosphate synthase genes provide high level glyphosate tolerance in tobacco}, volume={25}, ISSN={1365-313X}, url={http://dx.doi.org/10.1046/j.1365-313x.2001.00958.x}, DOI={10.1046/j.1365-313x.2001.00958.x}, number={3}, journal={The Plant Journal}, publisher={Wiley}, author={Ye, Guang‐Ning and Hajdukiewicz, Peter T.J. and Broyles, Debra and Rodriguez, Damian and Xu, Charles W. and Nehra, Narender and Staub, Jeffrey M.}, year={2001}, month=feb, pages={261–270} }

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