DOI: 10.1046/j.1365-313x.2000.00830.x. Identification of transposon‐tagged genes by the random sequencing of <i>Mutator</i>‐tagged DNA fragments from <i>Zea mays</i>

Identification of transposon‐tagged genes by the random sequencing of Mutator‐tagged DNA fragments from Zea mays

10.1046/j.1365-313x.2000.00830.x

Crossref journal-article

Wiley

The Plant Journal (311)

Abstract

SummaryWe have used a universal adaptor amplification procedure to isolate random Mutator‐tagged fragments from Mutator‐active maize plants. Direct sequence characterization of 761 Mutator‐tagged fragments indicated that a significant number were homologous to sequences within the public databases. The ability of Mutator‐tagged fragments to detect homology was not related to the length of the sequence within the range 100–400 bp. However, fragments above this size did show an increased chance of detecting homology to either expressed sequence tags or genes. Characterization of the insertion sites of the Mutator elements suggested that while it does target transcribed regions, Mutator does not appear to have any site preference within the transcription unit. Hybridization of previously unidentified Mutator‐tagged fragments to arrayed cDNA libraries confirmed that many of these also showed homology to transcribed regions of the genome. Examination of back‐crossed progeny confirmed that all the insertions examined were germinal; however, in all but one case, selfing five individual Mutator‐tagged lines failed to reveal an obvious phenotype. This study suggests that the random sequencing of Mutator‐tagged fragments is capable of producing both a significant number of interesting transposon tagged genes and mutant plant lines, all of which could be extremely valuable in future gene discovery and functional genomics programmes.

Bibliography

Hanley, S., Edwards, D., Stevenson, D., Haines, S., Hegarty, M., Schuch, W., & Edwards, K. J. (2000). Identification of transposonâtagged genes by the random sequencing of Mutatorâtagged DNA fragments from Zea mays. The Plant Journal, 23(4), 557â566. Portico.

Authors 7

Steven Hanley (first)
David Edwards (additional)
David Stevenson (additional)
Stephen Haines (additional)
Matthew Hegarty (additional)
Wolfgang Schuch (additional)
Keith J. Edwards (additional)

References 27 Referenced 45

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{'key': 'e_1_2_5_4_1', 'first-page': '183', 'volume-title': 'Plant Gene Systems and Their Biology, UCLA Symposia on Molecular and Cellular Biology New Series', 'author': 'Bennetzen J.L.', 'year': '1987'} / Plant Gene Systems and Their Biology, UCLA Symposia on Molecular and Cellular Biology New Series by Bennetzen J.L. (1987)
{'key': 'e_1_2_5_5_1', 'first-page': '75', 'article-title': 'Cloning and characterization of the maize An1 gene', 'volume': '7', 'author': 'Bensen R.J.', 'year': '1995', 'journal-title': 'Plant Cell'} / Plant Cell / Cloning and characterization of the maize An1 gene by Bensen R.J. (1995)
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{'key': 'e_1_2_5_12_1', 'volume-title': 'Molecular Cloning: a Laboratory Manual.', 'author': 'Maniatis T.', 'year': '1982'} / Molecular Cloning: a Laboratory Manual. by Maniatis T. (1982)
{'key': 'e_1_2_5_13_1', 'first-page': '181', 'volume-title': 'Molecular Tools for Screening Biodiversity: Plants and Animals (', 'author': 'Mathes M.', 'year': '1998'} / Molecular Tools for Screening Biodiversity: Plants and Animals ( by Mathes M. (1998)
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10.1093/genetics/117.4.771 / Genetics / Cloning of the Bronze2 locus of maize by transposon tagging and differential hybridisation by McLaughlin M. (1987)
{'key': 'e_1_2_5_16_1', 'first-page': '67', 'article-title': 'Reverse genetics for maize', 'volume': '69', 'author': 'Meeley R.B.', 'year': '1995', 'journal-title': 'Maize Genet. Newsl.'} / Maize Genet. Newsl. / Reverse genetics for maize by Meeley R.B. (1995)
{'key': 'e_1_2_5_17_1', 'first-page': '197', 'volume-title': 'Maize Handbook (', 'author': 'Neuffer M.G.', 'year': '1993'} / Maize Handbook ( by Neuffer M.G. (1993)
{'key': 'e_1_2_5_18_1', 'first-page': '1', 'article-title': 'Maize Genomics', 'volume': '2', 'author': "O'sullivan D.", 'year': '2000', 'journal-title': 'Agbiotechnet'} / Agbiotechnet / Maize Genomics by O'sullivan D. (2000)
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10.1093/genetics/143.3.1383 / Genetics / Mutator‐induced mutations of the rf1 nuclear fertility restorer of T‐cytoplasm maize alter the accumulation of T‐urf13 mitochondrial transcripts by Wise R.P. (1996)
10.1093/oxfordjournals.jhered.a111168 / J. Hered. / Use of a Ds chromosome‐breaking element to examine maize vp5 expression by Wurtzel E.T. (1992)

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 5:01 p.m.)
Deposited	1 year, 10 months ago (Oct. 29, 2023, 5:26 a.m.)
Indexed	1 year, 6 months ago (Feb. 21, 2024, 6:05 a.m.)
Issued	25 years, 1 month ago (Aug. 1, 2000)
Published	25 years, 1 month ago (Aug. 1, 2000)
Published Online	23 years, 8 months ago (Dec. 25, 2001)
Published Print	25 years, 1 month ago (Aug. 1, 2000)

Funders 0

None

BibTeX

@article{Hanley_2000, title={Identification of transposon‐tagged genes by the random sequencing of Mutator‐tagged DNA fragments from Zea mays}, volume={23}, ISSN={1365-313X}, url={http://dx.doi.org/10.1046/j.1365-313x.2000.00830.x}, DOI={10.1046/j.1365-313x.2000.00830.x}, number={4}, journal={The Plant Journal}, publisher={Wiley}, author={Hanley, Steven and Edwards, David and Stevenson, David and Haines, Stephen and Hegarty, Matthew and Schuch, Wolfgang and Edwards, Keith J.}, year={2000}, month=aug, pages={557–566} }

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